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产品简介
| 别名 | 番木瓜酶；木瓜酶；Papayotin; Papainase |
| 英文名称 | Papain |
| CAS | No.9001-73-4 |
| 分子量 | 21kD |
| 储存条件 | 2-8℃，有效期1年 |
| 外观(性状) |
白色粉末
|
| 级别 | 食品级 |
| 来源 | 番木瓜植物未成熟的果实 |
| 酶活/效价 | 80万u/g （以实际收到的标签酶活为准） |
| 溶解性 | 20mg/ml in water |
| 规格 | 25g 100g |
| 单位 | 瓶 |
说明：生化研究,用于抗体Fab片段的制备以及细胞分离。
性状：为乳白色至微黄色粉末，具有木瓜特有的气味,稍具有吸湿性。水解蛋白质能力强,但几乎不能分解蛋白胨，易溶于水,甘油,不溶于一般的有机溶剂,耐热性强
酶活力定义：紫外光分光度法：在测定条件（37±0.2℃；PH值7.0）每分钟水解酪蛋白释出的三氯乙酸可溶物在275nm波长有吸光度与1微克酪氨酸的吸光度相当时，所需的酶量即为一个活力单位，用u/g表示。一种能水解多肽、酰胺和酯的酶。其分子由212残基的折叠多肽键组成。微有吸湿性。对光敏感。不完全的溶于水和甘油，几乎不溶于多数有机溶剂。酶反应：裂解Arg-、Lys-、Phe-、X氨基酸。模型底物为苯甲酰精氨酸乙酯(BAEE)、N-苯甲酰-L-精氨酸对硝基苯胺(N-α-Bz-L-Arg-4-NA）、苄氧羰酰缬氨酰甘氨酰精氨酸对硝基苯胺(Chromozym TRY)。激活剂有半胱氨酸(Cys)、谷胱甘肽(GSH)、EDTA、还原剂、氰化物。抑制剂有汞(Hg2+)、其他重金属、碘乙酸、N-对基顺丁烯二
酶活：80万U/g
备注：产品信息可能会有优化升级。请以实际标签信息为准。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Gel Properties and Structural Characteristics of Composite Gels of Soy Protein Isolate and Silver Carp Protein
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Author：
Li Zheng, Joe M. Regenstein, Linyi Zhou, Sayed Mohamed Mokhtar, Zhongjiang Wang
IF：
4.4320
Publish_to：
Gels
PMID：
37233011
Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases
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Author：
Shihan Wang, Yuanshuai Gan, Xinxin Mao, Hong Kan, Nan Li, Changli Zhang, Zhihan Wang, Yongsheng Wang
IF：
4.4120
Publish_to：
MOLECULES
PMID：
33804057
Isolation, culture, and characterization of duck primary neurons.
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Author：
Sheng Yang, Jingxian Wu, Xuemei Xian, Qiusheng Chen
IF：
4.4000
Publish_to：
POULTRY SCIENCE
PMID：
36689785
The mechanical properties of tibiofemoral and patellofemoral articular cartilage in compression depend on anatomical regions
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Author：
Li Heng, Li Jinming, Yu Shengbo, Wu Chengwei, Zhang Wei
IF：
4.3800
Publish_to：
Scientific Reports
PMID：
33731799
Dual-phase SilMA hydrogel: a dynamic scaffold for sequential drug release and enhanced spinal cord repair via neural differentiation and immunomodulation
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Author：
Ruizhi Zhang, Mingzhe Zhang, Lu Chen, Linlin Jiang, Chenbo Zou, Na Li, Hengxing Zhou, Shiqing Feng
IF：
4.3000
Publish_to：
Frontiers in Bioengineering and Biotechnology
PMID：
39640066
LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration
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Author：
Yuqin Men, Aizhen Zhang, Haixiang Li, Yecheng Jin,
IF：
4.2590
Publish_to：
Scientific Reports 5, Article number: 16232 (2015)
PMID：
26549569
A novel bacteriocin against Staphylococcus aureus from Lactobacillus paracasei isolated from Yunnan traditional fermented yogurt: Purification, antibacterial characterization, and antibiofilm activity
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Author：
Yu-Hang Jiang, Wei-Gang Xin, Lin-Yu Yang, Jian-Ping Ying, Zi-Shun Zhao, Lian-Bing Lin, Xiu-Zhang Li, Qi-Lin Zhang
IF：
4.2250
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
35180941
Exploring the Utilization of Bovine Blood as a Source of Antioxidant Peptide: Production, Concentration, Identification, and In Silico Gastrointestinal Digestion
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Author：
Saruttiwong Boonkong, Pichitpon Luasiri, Jaksuma Pongsetkul, Saranya Suwanandgul, Sukanya Chaipayang, Wittawat Molee, Papungkorn Sangsawad
IF：
4.2000
Publish_to：
Food Science of Animal Resources
PMID：
39554827
Identification, molecular profiling and immune functions of cystatin M in silver pomfret (Pampus argenteus)
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Author：
Yadong Xue, Xiumei Liu, Yajun Wang, Jing Chang, Xubo Wang
IF：
4.1000
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
39168290
Effect of frozen storage on the quality and amylopectin structure of Chinese steamed bread made from different wheat cultivars
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Author：
Lili Deng, Jian Zhang, Hongtao Ren, Xiaoyan Song, Renyong Zhao
IF：
4.0750
Publish_to：
JOURNAL OF CEREAL SCIENCE
PMID：
An ECM-mimicking assembled gelatin/hyaluronic acid hydrogel with antibacterial and radical scavenging functions for accelerating open wound healing
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Author：
Xuebin Ma, Wenli Ning, Yiming Geng, Huarong Shao, Yang Liu, Fei Liu, Daizhou Zhang, Bo Chi, Yali Hou, Xiao Fu
IF：
4.0000
Publish_to：
Biomedical Materials
PMID：
37972551
Bioactive peptides derived from Radix Angelicae sinensis inhibit ferroptosis in HT22 cells through direct Keap1–Nrf2 PPI inhibition
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Author：
Ban Chen, Xiaojian Ouyang, Chunfeng Cheng, Dongfeng Chen, Jiangtao Su, Yuchen Hu, Xican Li
IF：
3.9000
Publish_to：
RSC Advances
PMID：
37492506
Comparative Analysis of Hyaline Cartilage Characteristics and Chondrocyte Potential for Articular Cartilage Repair
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Author：
Chiu Cheng, Zheng Kaiwen, Xue Mengxin, Du Dajiang
IF：
3.8000
Publish_to：
ANNALS OF BIOMEDICAL ENGINEERING
PMID：
38190025
Long non-coding RNA MEG3 regulates the progress of osteoarthritis by regulating the miR-34a/Klotho axis.
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Author：
Gaoxin Xiong, Shuangli Wang, Zhengjun Pan, Ning Liu, Donglei Zhao, Zhengang Zha, Rende Ning
IF：
3.6160
Publish_to：
Annals of Translational Medicine
PMID：
35571440
Natural product, bilobalide, improves joint health in rabbits with osteoarthritis by anti-matrix degradation and antioxidant activities
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Author：
Ma Tianwen, Chen Hong, Ruan Hongri, Lv Liangyu, Yu Yue, Jia Lina, Zhao Jinghua, Li Xin, Zang Yuxin, Xu Xinyu, Zhang Jiantao, Gao Li
IF：
3.4710
Publish_to：
Frontiers in Veterinary Science
PMID：
36337189
Design, biological characteristics, and antibacterial mechanism of high therapeutic index antimicrobial peptides with PRRP as central axis
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Author：
Yu Shuang, Jia Boyan, Zhang Ying, Yu Yue, Pei Zhihua, Ma Hongxia
IF：
3.3000
Publish_to：
JOURNAL OF ANTIBIOTICS
PMID：
38233466
Quantitative proteomic analysis of local and systemic extracellular vesicles during Eimeria falciformis infectious cycle in the host
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Author：
Olajide Joshua Seun, Qu Zigang, Yang Shunli, Yang Bin, Xu Xiao, Wang Jing, Cai Jianping
IF：
3.2000
Publish_to：
Parasites & Vectors
PMID：
37759313
Positive intervention of insoluble dietary fiber from defatted rice bran on hyperlipidemia in high fat diet fed rats
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Author：
Qian Liu, Jieyu Zhao, Sushi Liu, Yuchuan Fan, Jiajia Mei, Xuanjiang Liu, Tao Wei
IF：
3.1670
Publish_to：
JOURNAL OF FOOD SCIENCE
PMID：
34251041
Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different Proteases
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Author：
Liuhong Shen, Zeru Zhang, Yue Zhang, Yuquan Zhao, Lei Fan, Shumin Yu, Suizhong Cao, Yixin Huang
IF：
3.0000
Publish_to：
Animals
PMID：
37958152
A fast and efficient method for isolating and culturing enteric neural precursor cells from adult mouse colon
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Author：
Yifei Gao, Haojie Zhang, Jianchun Zhu, Jingxin Li, Yan Tang, Chuanyong Liu
IF：
2.9870
Publish_to：
JOURNAL OF NEUROSCIENCE METHODS
PMID：
36586440
Mechanical, nanomorphological and biological reconstruction of early stage apoptosis in HeLa cells induced by cytochalasin B
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Author：
Xuelian Su, Ling Zhang, Hong Kang, Baoping Zhang,
IF：
2.9760
Publish_to：
Oncology Reports Pages: 928-938
PMID：
30535459
Comparation of the structural characteristics and biological activities of chondroitin sulfates extracted from notochord and backbone of Chinese sturgeon (Acipenser sinensis)
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Author：
Dawei Meng, Xiaoqian Leng, Yan Zhang, Jiang Luo, Hao Du, Yasuaki Takagi, Zhiyuan Dai, Qiwei Wei
IF：
2.9750
Publish_to：
CARBOHYDRATE RESEARCH
PMID：
36209515
Effect of nanoprocessing on the physicochemical properties of bovine, porcine, chicken, and rabbit bone powders
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Author：
Xue Li, Zhifei He, Jingbing Xu, Ling Zhang, Yexing Liang, Shixiong Yang, Zefu Wang, Dong Zhang, Feihu Gao, Hongjun Li
IF：
2.8630
Publish_to：
Food Science & Nutrition
PMID：
34262719
Isolation and partial characterization of a novel bacteriocin from Pseudomonas azotoformans with antimicrobial activity against Pasterella multocida
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Author：
Wang Yu, Lei Shuang, Muhammad Inam, Jia Yu, He Cheng-guang, Wang Yi-Ming, Gao Yun-Hang, Kong Ling-Cong, Ma Hong-Xia
IF：
2.6670
Publish_to：
ARCHIVES OF MICROBIOLOGY
PMID：
34982208
Bone Marrow Mesenchymal Stem Cells–Derived Extracellular Vesicle miR-208a-3p Alleviating Spinal Cord Injury via Regulating the Biological Function of Spinal Cord Neurons
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Author：
Jianwei Yang, Yanhua Yao
IF：
2.6000
Publish_to：
DNA AND CELL BIOLOGY
PMID：
39133103
Effect of 3D printing technology-assisted TKA on cartilage tissue in rabbit with knee osteoarthritis.
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Author：
Wang G, Cheng F, Cui J, Chen N, Li Q
IF：
2.5000
Publish_to：
HISTOLOGY AND HISTOPATHOLOGY
PMID：
38639204
Resveratrol pretreatment protects neurons from oxygen–glucose deprivation/reoxygenation and ischemic injury through inhibiting ferroptosis
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Author：
Zhu Huimin, Huang Jiagui, Chen Yue, Li Xuemei, Wen Jun, Tian Mingfen, Ren Jiangxia, Zhou Li, Yang Qin
IF：
2.3370
Publish_to：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
PMID：
35357412
LncRNA Gm16638-201 Inhibits the 14-3-3Ɛ Pathway in the Murine Prefrontal Cortex to Induce Depressive Behaviors
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Author：
Ting Zhang, Wan Lun Wang, Tong Jia Liu, Shuang Lu, Yan Chao Bian, Rui Xiao, Chuan Ling Zhang
IF：
2.2640
Publish_to：
BIOLOGICAL & PHARMACEUTICAL BULLETIN
PMID：
36328497
Aging-Induced Down-Regulation of PKA/BKCa Pathway in Rat Cerebral Arteries
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Author：
N Li, R Shi, Y Ye, Y Zhang, Y Zhang, Z Wang, Y Gu, Y Yin, D Chen, J Tang
IF：
2.1390
Publish_to：
PHYSIOLOGICAL RESEARCH
PMID：
36426887
Notoginsenoside R1 restrains the proliferation and migration of airway smooth muscle cells isolated from rats with chronic obstructive pulmonary disease
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Author：
Xiaoyong Li, Kai Chen, Xuefei Shi, Shunli Dong, Yi Chen, Bin Wang
IF：
2.1000
Publish_to：
INHALATION TOXICOLOGY
PMID：
38411938
Melatonin enhances therapeutic outcomes of adipose tissue-derived mesenchymal stem cell therapy in rat osteoarthritis by reducing TNF-α and IL-1β-induced injuries
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Author：
Jiang Hao, Chen Jiafang, Lin Zhangya, Liao Naishun
IF：
2.0000
Publish_to：
CYTOTECHNOLOGY
PMID：
39188645
Grub polypeptide extracts protect against oxidative stress through the NRF2-ARE signaling pathway
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Author：
Jingyang Chen, Yingjian Sun, Shan Huang, Hong Shen, Yongjie Chen
IF：
1.8150
Publish_to：
Animal Cells and Systems
PMID：
35059140
Inhibition of lncRNA H19/miR-370-3p pathway mitigates neuronal apoptosis in an in vitro model of spinal cord injury (SCI)
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Author：
Xin Li, Yan Qian, Kaihua Tang, Yang Li, Rui Tao, Chunyan Gong, Li Huang, Kaiwen Zou, Lindong Liu
IF：
1.7570
Publish_to：
Translational Neuroscience
PMID：
33708438
技术问答
（6）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
京公网安备 11011202000391号
请问用于解离视神经组织，配液浓度多少合适?2023-11-01 16:22:17
您好，我们验证过该产品在水中的溶解性为20mg/ml，具体使用溶剂及浓度需要根据实验目的要求查阅相关文献进行参考。
这个酶的最佳适用pH和温度是多少呀，做酶解的话?2023-10-31 16:10:16
您好，木瓜蛋白酶的最佳适用pH为5.0-7.0，最适活性温度为50-60℃。
这个用前要水浴激活吗?请拨打17852274519告知急急急?2023-05-19 14:00:20
您好，不需要水浴激活；酶活定义：紫外光分光度法：在测定条件（37±0.2℃；PH值7.0）每分钟水解酪蛋白释出的三氯乙酸可溶物在275nm波长有吸光度与1微克酪氨酸的吸光度相当时，所需的酶量即为一个活力单位，用u/g表示。
请问该产品（木瓜蛋白酶）的最适ph是?2023-02-27 09:44:11
您好，本品最适合PH值5.7。
可以用来提取原代细胞嘛?2022-11-22 09:58:32
您好，可以的。