| CAS |
No.50-67-9 |
| 中文名称 |
5-羟基色胺 |
| 英文名称 |
Serotonin |
| 分子式 |
C10H12N2O |
| 分子量 |
176.22 |
| 溶解性 |
Soluble in DMSO |
| 纯度 |
≥98% |
| 级别 |
Cell Culture |
| 外观(性状) |
White to off-white Solid |
| 储存条件 |
Powder:-20℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year |
| 运输条件 |
冷藏运输 |
| 靶点 |
5-HT Receptor;COMT |
| 通路 |
Neuronal Signaling;GPCR & G Protein;Metabolic Enzyme&Protease |
| 背景说明 |
Serotonin 是 CNS 中的单胺神经递质和内源性 5-HT 受体激动剂。Serotonin 也是儿茶酚 O-甲基转移酶 (COMT) 的抑制剂。 |
| 生物活性 |
Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 μM.[1-2] |
| In Vitro |
Serotonin is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin also inhibits catechol O-methyltransferase (COMT)， an enzyme that contributes to modultion the perception of pain， via non-competitive binding to the site bound by catechol substrates with a binding affinity comparable to the binding affinity of catechol itself (Ki=?44 μM). Results show that addition of 100 μM of Serotonin decreases the reaction velocity of COMT[1]. |
| In Vivo |
Serotonin produces robust hypersensitivity compare to saline-treated controls (p[1]. A significant increase in colonic 5-HT content is observed in IL-13-/- mice receiving Serotonin compare to IL-13-/- receiving vehicle following induction of DSS colitis[2]. |
| 细胞实验 |
For macrophage culture， peritoneal cavity cells are collected from WT and IL-13-/- mice with or without dextran sodium sulfate (DSS) treatment and cultured. Cells are plated at a concentration of 3.0×106 cells per millilitre， and treated with either lipopolysaccharides (LPS); (100 ng/mL) or Serotonin (10-10 M) for 24 hours. The culture supernatant are collected and stored in -80°C until determination of cytokine levels using protein array system[2]. |
| 动物实验 |
Dextran sodium sulfate (DSS) colitis is induced by orally administering 5% DSS in drinking water for 5 days. In a separate experiment， IL-13-/- mice are injected subcutaneously with 100 mg/kg of Serotonin (5-HTP) twice daily for 8 days beginning 3 days prior to induction of DSS colitis; whereas， the control IL-13-/- mice receive saline as vehicle. Animals are anaesthetized prior to euthanization via cervical dislocation at the conclusion of each experiment or if they reach a predetermined end point (ie， loss of ≥20% body weight and/or significant deterioration of body condition)[2]. |
| 数据来源文献 |
[1]. Tsao D， et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25.
[2]. Shajib MS， et al. Interleukin 13 and serotonin: linking the immune and endocrine systems in murine models of intestinal inflammation. PLoS One. 2013 Aug 28;8(8):e72774. |
| 规格 |
1mg 5mg 10mg 25mg 50mg |
| 单位 |
瓶 |
中文
英语
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