| CAS |
No.121-33-5 |
| 中文名称 |
香兰素(10mM in DMSO,无菌） |
| 英文名称 |
Vanillin(10mM in DMSO，Sterile） |
| 分子式 |
C8H8O3 |
| 分子量 |
152.15 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Others |
| 通路 |
Others |
| 背景说明 |
Vanillin是一种酚醛，其官能团包括醛，羟基和醚。是一种具有生物活性的化合物。 |
| 生物活性 |
Vanillin (p-Vanillin) is a single molecule extracted from vanilla beans and also a popular odor used widely in perfume， food and medicine.[1-2] |
| In Vitro |
Vanillin 以剂量依赖的方式恢复 UVA 诱导的增殖减少。Vanillin 在测试浓度下没有凋亡作用。此外，由 UVA 照射引起的 OCT4、NANOG 和 SOX2 表达水平降低均通过 Vanillin 处理得到提高，表明 Vanillin 减弱了 UVA 照射对 hAMSCs 的影响。基于荧光素酶报告基因测定，Vanillin 增加了由 UVA 照射引起的 HRE-荧光素酶报告基因活性降低。此外，用 Vanillin 处理可减弱由 UVA 照射引起的 HIF-1α 表达降低。结果表明，与受 UVA 照射的对照相比，用 Vanillin 处理 hAMSCs 会显著减少 PGE2 和 cAMP 的产生[1]。 |
| In Vivo |
抗抑郁处理 4 周后，应激+香草醛组和应激+氟西汀组不动时间显著减少 [F (4，42) =34.73，P[2]。 |
| 细胞实验 |
hAMSCs are irradiated with the indicated doses of UVA and then incubated with 1 to 100 μM of Vanillin for three days under serum-free conditions (in DMEM devoid of serum， at 37°C with 5% CO2). After three days， cell proliferation is measured using BrdU incorporation assay[1]. |
| 动物实验 |
Male Sprague-Dawley rats (200 to 250 g) are used in this study. The animals are divided into three groups with 8 to 10 rats per group: the stress+fluoxetine group; the stress+Vanillin aromatherapy group and the stress (untreated) group. For the stress+fluoxetine group， the animals are administered a daily oral dose (10 mg/kg/d， diluted in distilled water) of the SSRI fluoxetine each morning. For the stress+Vanillin group and the bulbectomy+Vanillin group， Vanillin is administrated in a Plexiglas cylinder 50 cm tall and 35 cm diameter with two layers separated by a porous Plexiglas board. The rat still in its cage is gently placed on the upper layer， and 5 mL of 600 mg/L Vanillin (in distilled water) sprayed on to the floor of the lower layer. Rats in the stress and the control groups receive similar handling to the stress+Vanillin group， but without any odor administrated[2]. |
| 激酶实验 |
hAMSCs are irradiated with the indicated doses of UVA and then incubated with the indicated concentrations of Vanillin for three days under serum-free conditions. After the incubation， the concentrations of PGE2 or cAMP in the culture supernatant are measured using ELISA kits. Culture supernatants are added into 96 well plates. Alkaline phosphatase conjugated PGE2 or cAMP and antibodies to PGE2 or cAMP are added to sample wells and incubated at room temperature for 2 h. The sample wells are then washed with PBS and p-nitrophenyl phosphate (pNpp) substrate solution is added. Finally， the samples are incubated at room temperature for 1 h and their absorbance values are read according to the manufacturer‘s instructions[1]. |
| 数据来源文献 |
[1]. Lee SY， et al. Vanillin attenuates negative effects of ultraviolet A on the stemness of human adipose tissue-derived mesenchymal stem cells. Food Chem Toxicol. 2016 Oct;96:62-9.
[2]. Xu J， et al. Vanillin-induced amelioration of depression-like behaviors in rats by modulating monoamine neurotransmitters in the brain. Psychiatry Res. 2015 Feb 28;225(3):509-14. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
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