| CAS |
No.99-50-3 |
| 中文名称 |
3,4-二羟基苯甲酸(10mM in DMSO,无菌） |
| 英文名称 |
Protocatechuic acid(10mM in DMSO，Sterile） |
| 分子式 |
C7H6O4 |
| 分子量 |
154.12 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Others |
| 通路 |
Others |
| 背景说明 |
Protocatechuic acid 是一种多酚类物质，具有神经保护的活性。 |
| 生物活性 |
Protocatechuic acid is a phenolic compound which exhibits neuroprotective effect.[1-3] |
| In Vitro |
Protocatechuic acid 抑制 Aβ 和 αS 的聚集并破坏其预先形成的原纤维的稳定性。Protocatechuic acid 可防止由 Aβ 和 αS 诱导的毒性引发的 PC12 细胞死亡[3]。 |
| In Vivo |
Protocatechuic acid 能够在强迫游泳试验中防止应激诱导的不动时间，而不改变小鼠的运动活动。此外，Protocatechuic acid 处理可减轻 ARS 小鼠血清皮质酮升高、脂质过氧化并恢复大脑皮层和海马体中的酶促抗氧化剂[1]。给予镉并用前列斯的明和一定剂量的 Protocatechuic acid (10 -20 mg/kg) 处理的大鼠显著降低了 BChE 活性。镉和前列斯的明或 Protocatechuic acid (10 -20 mg/kg) 处理的大鼠显示 MDA 水平显著降低[2]。 |
| 细胞实验 |
Dilutions of Protocatechuic acid (2， 5， 10， 20， 50， and 100 μM) are prepared from stock solutions， with serum-free culture medium. Equal volumes of each solution are mixed with Aβ1-42 (10 μM)， then incubated for 24 h on a thermoblock， with continuous agitation， and then exposed to PC12 cells for 24 h to test whether Protocatechuic acid can prevent cell death triggered by Aβ. Cell viability is determined by MTT reduction assay. Cells are treated with 200 μL per well of MTT solution (final concentration， 0.5 mg/mL in DMEM-Glutamax medium) for 3 h， at 37°C， with 5% CO2. The dark blue formazan crystals that formed are solubilized with 100 μL per well of DMSO， for 30 min. Absorbance is measured at 540 nm， with a microplate reader. Results are expressed as the percentage of MTT reduction in relation to the absorbance of control cells at 100%. |
| 激酶实验 |
AChE activity investigation is carried out in a reaction mixture containing 50 μL of tissue homogenate， 50 μL of 5， 5‘-dithiobis-(2-nitrobenzoic) acid (DTNB)， 1175 μL of 0.1 M phosphate-buffered solution， pH 8.0. After incubation for 20 min at 25°C， 25 μL of acetylthiocholine iodide solution is added as the substrate. The AChE activity is determined as changes in absorbance reading at 412 nm for 3 min at 25°C and using a UV/Visible spectrophotometer. |
| 数据来源文献 |
[1]. Thakare VN， et al. Attenuation of acute restraint stress-induced depressive like behavior and hippocampal alterations with protocatechuic acid treatment in mice. Metab Brain Dis. 2016 Oct 26
[2]. Adefegha SA， et al. Alterations of Na+/K+-ATPase， cholinergic and antioxidant enzymes activity by protocatechuic acid in cadmium-induced neurotoxicity and oxidative stress in Wistar rats. Biomed Pharmacother. 2016 Oct;83:559-568
[3]. Hornedo-Ortega R， et al. Protocatechuic Acid: Inhibition of Fibril Formation， Destabilization of Preformed Fibrils of Amyloid-β and α-Synuclein， and Neuroprotection. J Agric Food Chem. 2016 Oct 10. |
| 规格 |
0.1ml 0.3ml 0.5ml 1ml 1.5ml |
| 单位 |
瓶 |
中文
英语
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