| CAS |
No.529-44-2 |
| 中文名称 |
杨梅素(10mM in DMSO,无菌） |
| 英文名称 |
Myricetin(10mM in DMSO，Sterile） |
| 分子式 |
C15H10O8 |
| 分子量 |
318.24 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Others |
| 通路 |
Others |
| 背景说明 |
Myricetin是一种类黄酮，具有强抗氧化，抗癌，抗糖尿病和抗炎活性。 |
| 生物活性 |
Myricetin is a common plant-derived flavonoid with a wide range of activities including strong anti-oxidant， anticancer， antidiabetic and anti-inflammatory activities.[1-5] |
| In Vitro |
Myricetin 对多种自由基和离子具有清除活性。它在超氧化物歧化酶 (SOD) 样活性测定[1]中表现出较差的活性 (IC50 值=1.4 mg/mL)。它通过调节 PI3K/Akt 和 MAPK 信号通路防止癌细胞因细胞凋亡而死亡[2]。 Myricetin 通过消除皮肤中的致病自由基而表现出抗光老化作用。Myricetin 能够抑制小鼠皮肤表皮 JB6 P+ 细胞中 UVB 诱导的 COX-2 表达。它抑制 UVB 诱导的激活蛋白 1 和 NF-κβ 的启动，以及 Fyn 激酶活性[1]。 Myricetin 以剂量依赖性方式抑制 SKOV3 卵巢癌细胞的活力。它诱导 DNA DSBs 和 ER 应激，从而导致 SKOV3 细胞凋亡[3]。 Myricetin 抑制人 Hsp70 超过 80%，IC50 值分别为 83、11 和 12 μM[4]。 |
| In Vivo |
用 Myricetin 处理原位胰腺肿瘤可使肿瘤消退并减少转移扩散[2]。暴露于 150 μM Myricetin 可分别抑制由 ADP、花生四烯酸、胶原蛋白和 PAF 诱导的兔血小板聚集 14%、26%、5% 和 49%[5]。 |
| 细胞实验 |
Pancreatic cancer cells (MIA PaCa-2， Panc-1 or S2-013) or normal pancreatic ductal cells (PDCs) are treated with myricetin (12.5–200 μM). Cell viability is determined using the Dojindo Cell Counting Kit-8. Cells are seeded onto a 96-well plate at 1×104 cells per well and allowed to adhere overnight. After treatment with myricetin at various concentrations for 24 hours， 10 μL of the tetrazolium substrate is added to each well of the plate. Plates are incubated at 37°C for 1 hour， after which the absorbance at 450 nm is measured[2]. |
| 动物实验 |
Mice: Mice are given daily intraperitoneal injections of myricetin (30mg/kg in the MIA PaCa-2 model and 50mg/kg in the S2-013 model) or vehicle (DMSO) for 35 days (MIA PaCa-2 model) or 18 days (S2-013 model). Ultrasound measurements are performed at regular intervals to monitor tumor growth. At the end of the in vivo experiment， tumor size is measured using calipers and tumor volume is calculated[2]. |
| 数据来源文献 |
[1]. Semwal DK， et al. Myricetin: A Dietary Molecule with Diverse Biological Activities. Nutrients. 2016 Feb 16;8(2):90.
[2]. Phillips PA， et al. Myricetin induces pancreatic cancer cell death via the induction of apoptosis and inhibition of thephosphatidylinositol 3-kinase (PI3K) signaling pathway. Cancer Lett. 2011 Sep 28;308(2):181-8.
[3]. Xu Y， et al. Myricetin induces apoptosis via endoplasmic reticulum stress and DNA double-strand breaks in human ovarian cancer cells. Mol Med Rep. 2016 Mar;13(3):2094-100.
[4]. Jinwal UK， et al. Chemical Manipulation of Hsp70 ATPase Activity Regulates Tau Stability. J Neurosci. 2009 Sep 30;29(39):12079-88.
[5]. Tzeng SH， et al. Inhibition of platelet aggregation by some flavonoids. Thromb Res. 1991 Oct 1;64(1):91-100. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
说明书下载
京公网安备 11011202000391号