| CAS |
No.84687-43-4 |
| 中文名称 |
黄芪甲苷(10mM in DMSO，无菌） |
| 英文名称 |
Astragaloside Ⅳ(10mM in DMSO，Sterile) |
| 分子式 |
C41H68O14 |
| 分子量 |
784.98 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 运输条件 |
冷冻运输 |
| 靶点 |
Others |
| 通路 |
Others |
| 背景说明 |
是一种具有生物活性的皂苷，可用于心血管，免疫，消化和神经系统等方向的研究。能够抑制ERK1/2和JNK的激活。 |
| 生物活性 |
Astragaloside IV， an active component isolated from Astragalus membranaceus， suppresses the activation of ERK1/2 and JNK， and downregulates matrix metalloproteases (MMP)-2， (MMP)-9 in MDA-MB-231 breast cancer cells.[1-4] |
| In Vitro |
Astragaloside IV (10， 20， 40 ng/mL) inhibits NSCLC cell growth， whereas low concentrations of astragaloside IV (1， 2.5， 5 ng/mL) has no obvious cytotoxicity on cell viability. Moreover， combined treatment with astragaloside IV significantly increases chemosensitivity to cisplatin in NSCLC cells. On the molecular level， astragaloside IV co-treatment significantly inhibits the mRNA and protein levels of B7-H3 in the presence of cisplatin[2]. Astragaloside IV inhibits the viability and invasive potential of MDA-MB-231 breast cancer cells， suppresses the activation of the mitogen activated protein kinase (MAPK) family members ERK1/2 and JNK， and downregulates matrix metalloproteases (MMP)-2 and -9[4]. |
| In Vivo |
Astragaloside IV (10， 20 mg/kg， p.o.) exhibits a potent ability to prevent cognitive deficits induced by transient cerebral ischemia and reperfusion. Astragaloside IV (10 mg/kg) and Astragaloside IV (20 mg/kg) can significantly decrease the levels of these cytokines compared to the Model group. Astragaloside IV significantly inhibits the level of TLR4 and its downstream proteins， suggesting that both MyD88-dependent and -independent pathways play important roles in the anti-inflammatory effects of Astragaloside IV. Astragaloside IV attenuates NLRP3 and cleaved-caspase-1 expression， and reduces Iba1 protein expression[1]. In the mice model， the high-dose astragaloside IV group has a significant increase in the 48-hour survival rate [60% (9/15) vs 13.3% (2/15)， P apoptosis of hepatocytes (P SOD[3]. |
| 细胞实验 |
Cell viability is determined by CCK-8 assay. To be brief， cultured NSCLC cells are seeded into 96-well plates at the density of 4×104 (cells/well). Then 10 μL?well CCK8 solution is added and incubated in dark at 37°C for another 2 h. The absorbance is determined with the wavelength of 490 nm.[2] |
| 动物实验 |
Transient cerebral ischemia and reperfusion is prepared by BCCAO， as BCCAO is considered an ideal model to study transient cerebral ischemia and reperfusion injury-mediated inflammatory response. Mice are randomLy divided into the Sham， Model， Astragaloside IV (10 mg/kg) and Astragaloside IV (20 mg/kg) treatment groups. The Astragaloside IV treatment groups are intragastrically administered 7 days before the surgery and terminated on the day of sacrifice. On the day of the surgery， Astragaloside IV is administrated 2 h prior to ischemia. The Sham-operated and Model groups are treated with distilled water. After the mice are anesthetized with an intraperitoneal injection of chloral hydrate (350 mg/kg)， the bilateral common carotid arteries are exposed and carefully separated with a small ventral neck incision and occluded twice (20 min each) with ligated surgical silk as described previously with minor modifications. There is a 10 min reperfusion period between the two occlusion periods (ischemia 20 min ? reperfusion 10 min ? ischemia 20 min). Sham-operated mice are subjected to the same surgical operation without the surgical silk ligation. Mouse body temperature is maintained at 37±0.5°C during the surgery with heating equipment until recovery from the anesthesia.[1] |
| 激酶实验 |
Briefly， MDA-MB-231 cells treated as indicated or tumor tissues are harvested and lysed in Mg2+ lysis buffer containing 50 mM Tris (pH 7.5)， 10 mM MgCl2， 0.5 M NaCl， and protease inhibitor cocktail. Equal amounts of lysates are incubated with PAK-PBD beads at 4°C for 1 h. PAK-PBD beads are pelleted by centrifugation and washed with ish buffer containing 25 mM Tris (pH 7.5)， 30 mM MgCl2， 40 mM NaCl. Active Rac1 is detected by western blotting.[4] |
| 数据来源文献 |
[1]. Li M， et al. Astragaloside IV attenuates cognitive impairments induced by transient cerebral ischemia and reperfusion in mice via anti-inflammatory mechanisms. Neurosci Lett. 2016 Dec 20. pii: S0304-3940(16)30994-
[2]. He CS， et al. Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3. Cell Physiol Biochem. 2016;40(5):1221-1229. Epub 2016 Dec 14.
[3]. Liu L， et al. [Protective effect of astragaloside IV against acute liver failure in experimental mice]. Zhonghua Gan Zang Bing Za Zhi. 2016 Oct 20;24(10):772-777
[4]. Jiang K， et al. Astragaloside IV inhibits breast cancer cell invasion by suppressing Vav3 mediated Rac1/MAPK signaling. Int Immunopharmacol. 2016 Dec 5;42:195-20 |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
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