| CAS |
No.16830-15-2 |
| 中文名称 |
积雪草苷(10mM in DMSO,无菌） |
| 英文名称 |
Asiaticoside(10mM in DMSO，Sterile） |
| 分子式 |
C48H78O19 |
| 分子量 |
959.12 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Others |
| 通路 |
Others |
| 背景说明 |
是一种三萜皂苷化合物。 |
| 生物活性 |
Asiaticoside， a trisaccaride triterpene from Centella asiatica， suppresses TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts; Asiaticoside shows antioxidant， anti-inflammatory， and anti-ulcer properties.[1-3] |
| In Vitro |
Asiaticoside (0、100、250 和 500 mg/L) 剂量依赖性地抑制瘢痕疙瘩成纤维细胞增殖。积雪草苷 (100、250 和 500 mg/L) 降低胶原蛋白和 mRNA 的表达，降低 TGF-bRI、TGF-bRII 蛋白和 mRNA 的表达，增加 Smad7 的表达，但不影响 Smad2、Smad3，瘢痕疙瘩成纤维细胞中的 Smad4、Smad2 和 Smad3 磷酸化[1]。Asiaticoside (12.5 和 50，以及 25 和 50 μg/mL) 可防止内皮细胞因缺氧诱导的细胞活力抑制和 NO 产生。Asiaticoside (50 μg/mL) 还可保护内皮细胞免受缺氧诱导的细胞凋亡，并在缺氧暴露的 HPAECs 中上调和磷酸化 AKT/eNOS[2]。 |
| In Vivo |
Asiaticoside (50 mg/kg/d) 可阻断肺动脉高压大鼠的缺氧性肺动脉高压 (PH)、心血管重塑和内皮细胞损伤的发展[2]。Asiaticoside (5、15 或 45 mg/kg，口服) 改善学习和记忆缺陷，保护海马免受损伤，减少海马中的 Aβ 沉积，并改善用 Aβ 寡聚体处理的大鼠受损的亚细胞结构[3]。 |
| 细胞实验 |
Cells are seeded at a density of 2-9 × 104 cells/mL into 24-well plates for cell viability， 60-mm plates for RNA and protein analysis in DMEM containing 10% FBS (DMEM/10% FBS). After 24 h， medium is removed and cells are placed in serum-free DMEM. After 48 h， different concentrations of Asiaticoside in DMEM/10% FBS are added simultaneously to cells. Control cells are grown in DMEM/10% FBS without the addition of Asiaticoside. At different time points， the fibroblasts are harvested for analysis[1]. |
| 动物实验 |
Rats[3]Sprague-Dawley rats are used to establish the Alzheimer‘s disease (AD) model. Asiaticoside is administered orally at 5， 15 or 45 mg/kg body weight per day for 7 days. The learning and memory function of the rats are evaluated by the MWM test 7 days after surgery. The swimming pool (180 cm in diameter) is maintained at 22-24°C， and divided into four quadrants with equal size. A hidden platform is placed in the center of one quadrant. Each of the cardinal points of these four quadrants is randomLy selected as the start location. On the pre-test day， rats are allowed to swim freely for 120 sec. During the test (days 1-4)， each rat is subjected to 8 trials each day. The trial begins when a rat is placed in the pool， and ended when the rat finds the platform. The escape latency is recorded. If a rat fails to find the platform within 120 sec， the trial is terminated and the escape latency is recorded as 120 sec， and the rat is guided to the platform. On day 5， the swimming path is recorded by a video recording system， and the digital images are analyzed by the water maze software. In addition， the probe test is performed， in which the platform is removed， and the rat is allowed to swim freely in the pool for 120 sec. The swimming time in the target quadrant is recorded[3]. |
| 数据来源文献 |
[1]. Tang B， et al. Asiaticoside suppresses collagen expression and TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts. h Dermatol Res. 2011 Oct;303(8):563-72.
[2]. Wang X， et al. Effect of asiaticoside on endothelial cells in hypoxia?induced pulmonary hypertension. Mol Med Rep. 2018 Feb;17(2):2893-2900.
[3]. Zhang Z， et al. Asiaticoside ameliorates β-amyloid-induced learning and memory deficits in rats by inhibiting mitochondrial apoptosis and reducing inflammatory factors. Exp Ther Med. 2017 Feb;13(2):413-420. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
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