| CAS |
No.501-36-0 |
| 中文名称 |
白藜芦醇(10mM in DMSO,无菌） |
| 英文名称 |
Resveratrol(10mM in DMSO，Sterile） |
| 分子式 |
C14H12O3 |
| 分子量 |
228.24 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
IKK |
| 通路 |
NF-κB |
| 背景说明 |
Resveratrol是一种多酚类植物抗毒素，具有抗氧化，抗炎，保护心脏和抗癌等活性。 Resveratrol的靶点广泛，如 mTOR、JAK、β-amyloid、 COX、LOC、Nrf2、PXR等。Resveratrol 也是一种特异性的 SIRT1 活化剂。 |
| 生物活性 |
Resveratrol (trans-Resveratrol; SRT501)， a natural polyphenolic phytoalexin that possesses anti-oxidant， anti-inflammatory， cardioprotective， and anti-cancer properties. Resveratrol (SRT 501) has a wide spectrum of targets including mTOR， JAK， β-amyloid， Adenylyl cyclase， IKKβ， DNA polymerase. Resveratrol also is a specific SIRT1 activator. Resveratrol is a potent pregnane X receptor (PXR) inhibitor. Resveratrol is an Nrf2 activator， ameliorates aging-related progressive renal injury in mice model. Resveratrol increases production of NO in endothelial cells.[1-7] |
| In Vitro |
Resveratrol (trans-Resveratrol; SRT501) 是在多种植物来源中发现的众多多酚化合物之一 在绝大多数情况下，Resveratrol 在微摩尔范围内显示出抑制/激活作用，这在药理学上可能是可以实现的，尽管目标在还报道了纳摩尔范围[1]。将 MCF-7 细胞接种在添加了 5% FBS 且 Resveratrol 浓度不断增加的 DME-F12 培养基中。对照细胞仅用相同体积的载体 (0.1% 乙醇) 处理。Resveratrol 以剂量依赖性方式抑制 MCF-7 细胞的生长。添加 10 μM Resveratrol 导致 6 天后 82% 的 MCF-7 细胞生长抑制，而在 1 μM 时，仅观察到 10% 的抑制。用 10 μM Resveratrol 处理的细胞的倍增时间为 60 小时，而对照细胞每 30 小时倍增一次。台盼蓝排斥试验表明，在 10 μM 或更低的浓度下，Resveratrol 不会影响细胞活力 (90% 的活细胞)，而在 100 μM 的浓度下，Resveratrol 处理 6 天后只有 50% 的细胞存活。此外，根据 ApoAlert Annexin V 细胞凋亡试剂盒的测定，MCF-7 细胞在与浓度为 10 μM 的 Resveratrol 孵育后不会发生细胞凋亡[2]。 Resveratrol 通过上调内皮一氧化氮合酶 (eNOS) 的表达、刺激 eNOS 酶活性和阻止 eNOS 解偶联来增加内皮细胞中一氧化氮 (NO) 的产生[7]。 |
| In Vivo |
Resveratrol (trans-Resveratrol；SRT501) 以 50 mg/kg (195.5±124.8 mm3；P3) 相比，平均肿瘤体积减小(81.7±70.5 mm3；P[3]。 |
| 细胞实验 |
To determine the effect of Resveratrol on cell growth， MCF-7 cells are plated in 6-well plates at 105 cells per well in 2 mL of DME-F12 medium supplemented with 5% FBS in the presence or absence of increasing concentrations of Resveratrol. The cell number is measured every 2 days till day 6 with a hemocytometer after detaching the cells with trypsin-EDTA[2]. |
| 动物实验 |
Mice[3]Female BALB/c (nu/nu) mice， 6 weeks old， are used. PA-1 cells (1×107 in 200 μL PBS) are injected s.c. on the right hind flank. Tumor volume (length×width×depth×0.52) is measured three times a week. After 10 days of implantation， two groups (n=10) are given Resveratrol (dissolved in 5% ethanol and 25% polyethyleneglycol 400 in distilled water) i.p. at a daily dose of 50 or 100 mg/kg body weight for consecutive 4 weeks， whereas the other group receive the vehicle only. Body weights are recorded everyday. Animals are given bromodeoxyuridine (BrdUrd; 10 mg/kg body weight， i.p.) 2 h before sacrifice. Xenograft tumors are weighed and frozen in liquid nitrogen or fixed in 10% formalin and embedded in paraffin. The BrdUrd-labeled cells in paraffin-embedded tissues are detected employing a monoclonal anti-BrdUrd antibody. |
| 数据来源文献 |
[1]. Pirola L， et al. Resveratrol: one molecule， many targets. IUBMB Life. 2008 May;60(5):323-32.
[2]. Lu R， et al. Resveratrol， a natural product derived from grape， exhibits antiestrogenic activity and inhibits the growth of human breast cancer cells. J Cell Physiol. 1999 Jun;179(3):297-304.
[3]. Lee MH， et al. Resveratrol suppresses growth of human ovarian cancer cells in culture and in a murine xenograft model: eukaryotic elongation factor 1A2 as a potential target. Cancer Res. 2009 Sep 15;69(18):7449-58.
[4]. Du LL， et al. Activation of sirtuin 1 attenuates cerebral ventricular streptozotocin-induced tau hyperphosphorylation and cognitive injuries in rat hippocampi. Age (Dordr). 2014 Apr;36(2):613-23.
[5]. Smutny T， et al. Resveratrol as an inhibitor of pregnane X receptor (PXR): another lesson in PXR antagonism. J Pharmacol Sci. 2014;126(2):177-8.
[6]. Eun Nim Kim，et al. Resveratrol， an Nrf2 activator， ameliorates aging-related progressive renal injury. Aging (Albany NY). 2018 Jan; 10(1): 83–99.
[7]. Huige Li， et al. Resveratrol and Vascular Function. Int J Mol Sci. 2019 Apr 30;20(9):2155. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
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