| CAS |
No.2292-16-2 |
| 中文名称 |
甲基莲心碱(10mM in DMSO,无菌） |
| 英文名称 |
Neferine(10mM in DMSO，Sterile） |
| 分子式 |
C38H44N2O6 |
| 分子量 |
624.77 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
NF-κB |
| 通路 |
NF-κB |
| 背景说明 |
Neferine 是一种双苄基异喹啉类生物碱，能够强效抑制 NF-κB 激活。 |
| 生物活性 |
Neferine is a major bisbenzylisoquinline alkaloid. Neferine strongly inhibits NF-κB activation.[1-2] |
| In Vitro |
Neferine down regulates hypoxia induced NF-κB p65 nuclear translocation and COX-2 expressions[1]. Neferine reduces high-glucose-induced collagen production and inhibits TGF-β1-Smad， ERK and p38 MAPK signaling activation in cardiac fibroblasts. Cardiac fibroblasts (CFs) are cultured in HG medium with varying concentrations of Neferine (1， 2， or 5 μM). CCK-8 assays are carried out at different time points (24， 48， and 72 h). Compared with normal glucose (NG) and osmotic control (OC) treatments， High glucose (30 mM) treatment significantly increases the proliferation of CFs in a time-dependent manner (P[2]. |
| In Vivo |
Neferine treatment at both low-dose (60 mg/kg/day by gavage) and high-dose (120 mg/kg/day by gavage) reduces the increment of collagen I， III and TGF-β1 protein expression induced by hyperglycemia[2]. |
| 细胞实验 |
Cardiac fibroblasts (CFs) are isolated from neonatal mouse ventricular tissues. After starvation in serum-free medium for 24 h， CFs are incubated in DMEM containing 5.6 mM glucose (normal glucose; NG)， 30 mM D-glucose (HG)， 30 mM D-glucose plus 1 μM Neferine， 30 mM D-glucose plus 2 μM Neferine， 30 mM D-glucose plus 5 μM Neferine， and 5.6 mM glucose plus 27.5 mM mannose. Cells are harvested at 24 h， 48 h， and 72h. Cell proliferation is measured with the Cell Counting Kit-8 (CCK-8) and the Cell-LightTM EdU assay[2]. |
| 动物实验 |
Mice[2]Eight-week-old C57BL/6J male mice are used. Diabetes is induced by intraperitoneal injection of Streptozotocin dissolved in citrate buffer (pH 4.5) at 60 mg/kg body weight for five consecutive days. Control mice are injected with citrate buffer only. Whole blood glucose in mouse tail blood is detected with an Accu-Check Active glucometer. Mice with blood glucose concentrations higher than 18 mM are considered as diabetic animals and used in this study. The animals are randomly divided into four groups of eight animals each. Diabetic mice are divided into three groups: group 1， the diabetic control group (DM); group 2， which receive Neferine at a dose of 60 mg/kg/day (DM-NL); and group 3， which receive Neferine at a dose of 120 mg/kg/day (DM-NH). Neferine is administered twice per day by intragastric gavage for 12 weeks. Equivalent volumes of normal sodium are administered to the normal and DM control groups by gavage. Mice are anaesthetized and sacrificed at the end of the 12-week treatment[2]. |
| 数据来源文献 |
[1]. Baskaran R， et al. Neferine prevents NF-κB translocation and protects muscle cells from oxidative stress and apoptosis induced by hypoxia. Biofactors. 2016 Jul 8;42(4):407-17.
[2]. Liu X， et al. Neferine inhibits proliferation and collagen synthesis induced by high glucose in cardiac fibroblasts and reduces cardiac fibrosis in diabetic mice. Oncotarget. 2016 Sep 20;7(38):61703-61715. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
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