| CAS |
No.1077-28-7 |
| 中文名称 |
DL-α-硫辛酸(10mM in DMSO,无菌） |
| 英文名称 |
α-Lipoic Acid(10mM in DMSO，Sterile） |
| 分子式 |
C8H14O2S2 |
| 分子量 |
206.33 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
NF-κB |
| 通路 |
NF-κB |
| 背景说明 |
是一种抗氧化剂，是线粒体酶复合物的重要辅助因子。可抑制 NF-κB 依赖性的 HIV-1 LTR活化。 |
| 生物活性 |
α-Lipoic Acid (Thioctic acid) is an antioxidant， which is an essential cofactor of mitochondrial enzyme complexes. α-Lipoic Acid inhibits NF-κB-dependent HIV-1 LTR activation. α-Lipoic Acid induces endoplasmic reticulum (ER) stress-mediated apoptosis in hepatoma cells. α-Lipoic Acid can be used with CPUL1 (HY-151802) to construct the self-assembled nanoaggregate CPUL1-LA NA， which has improved antitumor efficacy than CPUL1.[1-5] |
| In Vitro |
The long terminal repeat (LTR) of HIV-1 is the target of cellular transcription factors such as NF-κB， and serves as the promoter-enhancer for the viral genome when integrated in host DNA[1]. α-Lipoic Acid (Alpha-Lipoic acid， ALA)， a naturally occurring dithiol compound， plays an essential role in mitochondrial bioenergetics. α-Lipoic Acid reduces lipid accumulation in the liver by regulating the transcriptional factors SREBP-1， FoxO1， and Nrf2， and their downstream lipogenic targets via the activation of the SIRT1/LKB1/AMPK pathway. Treatment of cells with α-Lipoic Acid (250， 500 and 1000 μM) significantly increases the NAD+/NADH ratio in HepG2 cells (PSIRT1 activity in HepG2 cells. α-Lipoic Acid (50， 125， 250， 500 and 1000 μM) increases phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) in HepG2 cells in a dose-dependent fashion[1]. |
| In Vivo |
C57BL/6J mice， divided into four groups， are fed an high-fat diet (HFD) for 24 weeks to induce nonalcoholic fatty liver disease (NAFLD) followed by daily administration of α-Lipoic Acid. Then， the effects of α-Lipoic Acid on hepatic lipid accumulation in long-term HFD-fed mice are assessed. Administration of α-Lipoic Acid (100 mg/kg or 200 mg/kg) markedly reduces visceral fat mass in mice. In addition， α-Lipoic Acid (100 mg/kg or 200 mg/kg) treatment inhibits the appetite and causes a dramatic weight loss (all P[1]. |
| 细胞实验 |
The human hepatocellular carcinoma (HepG2) cell line is cultured in Dulbecco‘s modified Eagle‘s medium containing 10% fetal bovine serum at 37°C and 5% CO2. HepG2 cells are treated with AMPK inhibitor (CC， 20 μM， 0.5 h)， SIRT1 inhibitor (NA， 10 mM， 12 or 24 h)， and AMPK activator (AICAR， 2 mM， 1 h)， Palmitate (PA， 125 μM， 12 h) and α-Lipoic Acid (250 μM， 6 or 12 h)[1]. |
| 动物实验 |
Mice[1]Male C57BL/6J mice (6-week-old; body weight: 22-24 g) are allowed ad libitum access to normal diet and water for 2 weeks before dividing into four groups (n=8): normal diet (ND) (10% energy from fat)， high-fat diet (HFD) (60% energy from fat) and HFD plus α-Lipoic Acid (100 mg/kg or 200 mg/kg). After 24 weeks of treatment， blood samples are collected after the eyeballs of the mice are extracted for serum preparation by centrifugation at 2000×g for 10 min at 4°C. The liver tissues are harvested in liquid nitrogen and stored at -80°C. |
| 数据来源文献 |
[1]. Xiao L， et al. Activity of the dietary antioxidant ergothioneine in a virus gene-based assay for inhibitors of HIV transcription. Biofactors. 2006;27(1-4):157-65.
[2]. Lei D， et al. Synergistic neuroprotective effect of rasagiline and idebenone against retinal ischemia-reperfusion injury via the Lin28-let-7-Dicer pathway. Oncotarget. 2018 Jan 30;9(15):12137-12153.
[3]. Yang Y， et al. Alpha-lipoic acid improves high-fat diet-induced hepatic steatosis by modulating the transcription factors SREBP-1， FoxO1 and Nrf2 via the SIRT1/LKB1/AMPK pathway. J Nutr Biochem. 2014 Nov;25(11):1207-1217.
[4]. Pibiri M， et al. α-Lipoic acid induces Endoplasmic Reticulum stress-mediated apoptosis in hepatoma cells. Sci Rep. 2020 Apr 28;10(1):7139.
[5]. Liu J， et al. Nanoaggregates of Disulfide-Decorated TrxR Inhibitor Promote Cellular Uptake， Selective Targeting， and Antitumor Efficacy. Langmuir， 2022. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
说明书下载
京公网安备 11011202000391号