| CAS |
No.52286-74-5 |
| 中文名称 |
人参皂苷Rg2(10mM in DMSO,无菌） |
| 英文名称 |
Ginsenoside Rg2(10mM in DMSO，Sterile） |
| 分子式 |
C42H72O13 |
| 分子量 |
785.01 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
NF-κB |
| 通路 |
NF-κB |
| 背景说明 |
Ginsenoside Rg2是一种 NF-κB 抑制剂。还降低 Aβ1-42 积聚。 |
| 生物活性 |
Ginsenoside Rg2 is one of the major active components of ginseng. Ginsenoside Rg2 inhibits VCAM-1 and ICAM-1 expressions stimulated with lipopolysaccharide (LPS). Ginsenoside Rg2 also reduces Aβ1-42 accumulation.[1-3] |
| In Vitro |
Ginsenoside Rg2 prevents the decrease of IκB expression stimulated with lipopolysaccharide (LPS). IκB dissociation from RelA-p50 complex is crucial for NF-κB activity. Ginsenoside Rg2， protopanaxatriol， inhibits vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) expression stimulated with LPS from human umbilical vein endothelial cell (HUVEC). The inhibition of VCAM-1 and ICAM-1 expression by Ginsenoside Rg2 is in a concentration-dependent manner， significantly. Treatment of endothelial cells with LPS (1μg/mL) decreases IκBα expression. By 1 hr after LPS treatment， significant decrease of IκBα is attained. To determine whether LPS-stimulated IκBα expression is affected by Ginsenoside Rg2， endothelial cells are treated for 1 hr with Ginsenoside Rg2 (1~50 μM) prior to LPS (1 μg/mL) stimulation for 1 hr. Ginsenoside Rg2 reverses the decrease of LPS-induced IκBα expression in a concentration-dependent manner， significantly. The adhesion of THP-1 cells to endothelial cells is measured using quantitative monolayer adhesion assay. The adhesion of THP-1 cells onto endothelial cells are increased to five folds by LPS (1 μg/mL) stimulation for 8 hrs. Ginsenoside Rg2 (1~50 μM) inhibits the adhesion of THP-1 cells to endothelial cells stimulated with LPS， in a concentration-dependent manner[1]. |
| In Vivo |
G-Rg1 and Ginsenoside Rg2 (G-Rg2) reduce the escape latencies on the last two training days compared to the Alzheimer‘s disease (AD) model group (p1-42 accumulation in APP/PS1 mice. In the G-Rg1 and Ginsenoside Rg2 treated mice， the pathological abnormalities observed in the APP/PS1 mice are gradually ameliorated. Clear nucleoli and light brown， sparsely scattered Aβ deposits are visible[2]. |
| 细胞实验 |
HUVECs ares grown in EBM-2 containing 10% FBS at a density of 2.0×105 cells/well on 24-well plates. Endothelial cells at 90~95% confluence are treated with Ginsenoside Rg2 (1， 20， 50 μM) for 1 hr prior to 1 μg/mL of LPS stimulation for 8 hr. THP-1 cells are labeled with Calcein-AM (5 μM) in RPMI 1640 medium containing 10% FBS for 30 min. After extensive washing with PBS， the labeled THP-1 cells are seeded at a density of 5.0×105 cells/well onto endothelial cells which are treated with the Rg2 and/or LPS and， then， incubated for 1 hr at 37°C while gentle shaking. After incubation， non-adherent cells are removed by gentle washing two times with PBS. Photograph images are obtained at 485 nm excitation and 538 nm emission using a SPOT II digital camera-attached fluorescence microscope[1]. |
| 动物实验 |
Mice[2] Male APP/PS1 mice， weighing 20±2 g， and male C57BL/6J mice， weighing 20±2 g， are used. The animals are maintained in an air-conditioned animal center at 23±2°C and a relative humidity of 50±10%， with a natural light-dark cycle. Food and water are available ad libitum. After acclimatization for 1 wk， the mice are divided into four groups (n=10 in each group): the normal control group， the AD model group， the G-Rg1 group， and the Ginsenoside Rg2 group. According to the concentration-response curves， the mice in the G-Rg1 and Ginsenoside Rg2 groups are injected intraperitoneally once daily with G-Rg1 and Ginsenoside Rg2 (30 mg/kg)， respectively， dissolved in saline. The mice in the AD model group (APP/PS1 mice) and the normal control group (C57BL/6J nontransgenic littermates) are treated with isodose saline (0.9% w/v). All mice are treated for 1 mo before brain metabolite profiling. |
| 数据来源文献 |
[1]. Cho YS， et al. Ginsenoside rg2 inhibits lipopolysaccharide-induced adhesion molecule expression in human umbilical vein endothelial cell. Korean J Physiol Pharmacol. 2013 Apr;17(2):133-7.
[2]. Li N， et al. A UPLC/MS-based metabolomics investigation of the protective effect of ginsenosides Rg1 and Rg2 in mice with Alzheimer‘s disease. J Ginseng Res. 2016 Jan;40(1):9-17.
[3]. Cho YS， et al. Ginsenoside rg2 inhibits lipopolysaccharide-induced adhesion molecule expression in human umbilical vein endothelial cell. Korean J Physiol Pharmacol. 2013;17(2):133-137. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
说明书下载
京公网安备 11011202000391号