| CAS |
No.520-34-3 |
| 中文名称 |
香叶木素(10mM in DMSO,无菌） |
| 英文名称 |
Diosmetin(10mM in DMSO，Sterile） |
| 分子式 |
C16H12O6 |
| 分子量 |
300.26 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Cytochrome P450 |
| 通路 |
Metabolic Enzyme&Protease |
| 背景说明 |
Diosmetin是芳烃受体(AHR)的激动剂。 |
| 生物活性 |
Diosmetin is a natural flavonoid which inhibits human CYP1A enzyme activity with an IC50 of 40 μM in HepG2 cell.[1-2] |
| In Vitro |
Diosmetin 以浓度依赖性方式抑制 HepG2 细胞的细胞增殖。未经处理的 HepG2 细胞生长良好，骨架正常，而经 Diosmetin 处理的细胞发生变形，部分细胞呈圆形漂浮状态[1]。 |
| In Vivo |
用 Diosmetin 预处理可显著降低淀粉酶和脂肪酶的血清水平；组织损伤；肿瘤坏死因子 (TNF)-α、白细胞介素 (IL)-1β 和 IL-6 的分泌；髓过氧化物酶 (MPO) 活性、胰蛋白酶原激活肽 (TAP) 水平、诱导型一氧化氮合酶 (iNOS) 的表达；和核因子 (NF)-κB 在雨蛙素诱导的急性胰腺炎中的激活[2]。 |
| 细胞实验 |
Diosmetin is dissolved in DMSO which is maintained at a constant concentration in control samples (2%). HepG2 cells are maintained in a humidified atmosphere of 5% CO2 at 37°C， and cultured in RPMI-1640 medium supplemented with 10% (v/v) fetal bovine serum， 100 U/mL penicillin and 100 U/mL streptomycin. HepG2 cell density is adjusted to 2×104 cells/100 μL， and the cells are seeded into 96-well plates and placed in an incubator overnight (37°C in 5% CO2) to allow for attachment and recovery. MTT analyses are performed. Briefly， cells are pretreated with 5， 10， 15 and 20 μg/mL diosmetin for 24 h. A total of 20 μL MTT solution (5 mg/mL in PBS) solution is transferred to each well to yield a final 120 μL/well and to separate wells a total of 10 μL CCK8 (5 mg/mL in PBS) is transferred. The plates are incubated for 4 h at 37°C in 5% CO2 and the absorbance is recorded at wavelengths of 595 nm and 450 nm， respectively. The half maximal inhibitory concentration (IC50) of diosmetin is calculated[1]. |
| 动物实验 |
Experimental acute pancreatitis is induced in mice by seven intraperitoneal injection of cerulein (50 μg/kg) at hourly intervals. Diosmetin (100 mg/kg) or vehicle is pretreated 2 h before the first cerulein injection. After 6 h， 9 h， 12 h of the first cerulein injection， the severity of acute pancreatitis is evaluated biochemically and morphologically[2]. |
| 数据来源文献 |
[1]. Liu B， et al. Diosmetin induces apoptosis by upregulating p53 via the TGF-β signal pathway in HepG2 hepatoma cells. Mol Med Rep. 2016 Jul;14(1):159-64.
[2]. Yu G， et al. Diosmetin ameliorates the severity of cerulein-induced acute pancreatitis in mice by inhibiting the activation of the nuclear factor-κB. Int J Clin Exp Pathol. 2014 Apr 15;7(5):2133-42. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
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