| CAS |
No.16009-13-5 |
| 中文名称 |
氯化血红素(10mM in DMSO,无菌） |
| 英文名称 |
Chlorohemin(10mM in DMSO，Sterile） |
| 分子式 |
C34H32ClFeN4O4 |
| 分子量 |
651.94 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Heme oxygenase (HO-1) |
| 通路 |
Metabolic Enzyme&Protease |
| 背景说明 |
Chlorohemin是含铁的卟啉化合物和血红素加氧酶 (HO)-1 诱导剂。 |
| 生物活性 |
Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer.[1-2] |
| In Vitro |
Hemin and PGJ2， used as positive controls， strongly increase both expression and activity of HMOX after 4 and 12 h， respectively. Indeed， a significant effect is found of 30 μM Hemin on cell proliferation in all used cell lines after 48 h， which is dose-dependent. Hemin treatment decreases cell proliferation to 62±5 %， 51±3 %， and 38±8 % in PA-TU-8902， BxPC-3 and MiaPaCa-2 cancer cells， respectively， with p[1]. |
| In Vivo |
Following the i.p. administration of Hemin (100 μmol/kg)， the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after Hemin preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following Hemin preconditioning[2]. |
| 细胞实验 |
For the cell proliferation assay， cells are seeded into 96 well (5-12.5×104 cells per mL according to the cell line) and kept at 37°C and 5 % CO2. After 24 h， cells are treated with statins or/and Hemin， followed by the MTT test as a general cell proliferation assay[1]. |
| 动物实验 |
Mice[2] Eight- to ten-week-old male BABL/c mice are used for the ischemia-reperfusion (I/R) experiments. The animals are divided into five groups as follows: (1) the sham group undergo isolation of the bilateral renal arteries without clamping; (2) the vehicle group receive an intraperitoneal (i.p.) injection of 4 mL/kg PBS as a vehicle control (with IRI); (3) the Hemin-preconditioned group receive Hemin， a potent inducer of HO-1， at 100 μmol/kg i.p.; (4) the Hemin plus ZnPP group receive zinc protoporphyrin IX， an inhibitor of HO-1 activity， at 5 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p.; and (5) the Hemin plus PD98059 group receive PD98059， an inhibitor of ERK1/2 activity， at 10 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p. Both inhibitors are administered i.p. 2 h before I/R， whereas Hemin was administered 8 h before I/R. |
| 数据来源文献 |
[1]. Vanova K， et al. Heme oxygenase is not involved in the anti-proliferative effects of statins on pancreatic cancer cells. BMC Cancer. 2016 May 12;16:309.
[2]. Chen HH， et al. Heme oxygenase-1 ameliorates kidney ischemia-reperfusion injury in mice through extracellular signal-regulated kinase 1/2-enhanced tubular epithelium proliferation. Biochim Biophys Acta. 2015 Oct;1852(10 Pt A):2195-201. |
| 规格 |
1ml |
| 单位 |
瓶 |
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