| CAS |
No.491-67-8 |
| 中文名称 |
黄芩素(10mM in DMSO,无菌） |
| 英文名称 |
Baicalein(10mM in DMSO，Sterile） |
| 分子式 |
C15H10O5 |
| 分子量 |
270.24 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Xanthine Oxidase（XO） |
| 通路 |
Metabolic Enzyme&Protease |
| 背景说明 |
是黄嘌呤氧化酶抑制剂。 |
| 生物活性 |
Baicalein (5，6，7-Trihydroxyflavone) is a xanthine oxidase inhibitor with an IC50 value of 3.12 μM.[1-4] |
| IC50 |
IC50: 3.12 μM (xanthine oxidase)[1] |
| In Vitro |
Baicalein 体外抑制有丝分裂原诱导的 T 细胞增殖和细胞因子分泌。在 25 μM 时，用 Baicalein 预处理可显著抑制 Con A 或抗 CD3/CD28 mAb 诱导的增殖和细胞因子分泌。Baicalein 处理可诱导 NF-κB 与 DNA 结合，但会抑制核区室中的硫氧还蛋白活性[2]。Baicalein 以时间和剂量依赖性方式抑制 MDA-MB-231 细胞的增殖、迁移和侵袭。Baicalein 显著降低 MDA-MB-231 细胞中 SATB1 的表达。Baicalein 还下调 Wnt1 和 β-catenin 蛋白的表达以及 Wnt/β-catenin 靶向基因的转录水平[3]。 |
| In Vivo |
Baicalein 抑制移植物抗宿主病的诱导，但不抑制小鼠 T 细胞的稳态增殖。这一观察清楚地表明 Baicalein 在体内具有强大的抗炎活性[2]。用 Baicalein 处理的大鼠可防止心脏体重比、脑利钠肽血浆水平、室间隔厚度、左心室心肌胶原体积增加 (分别为 P [4]。 |
| 细胞实验 |
MTT assay is conducted to evaluate the effect of baicalein on proliferation of breast cancer cells. MDA-MB-231 cells are routinely digested， collected， and then seeded in 96-well plates at a density of 8×103 cells/well. After incubation for 12-24 hours， cells are treated with 0， 20， 40， 60， 80， 100， and 120 μM baicalein according to their experimental grouping and then incubated at 37°C for 24， 48， and 72 hours[3]. |
| 动物实验 |
Rats: Baicalein is suspended in 1% methylcellulose. Rats are treated with baicalein suspension via oral garvage. SHR and WKY rats are divided into 4 groups (n=8 per group): 12-week treatment with high-dose (200 mg/kg/day) or low-dose (50 mg/kg/day) group; and 4-week treatment with high-dose or low-dose group. The 12-week and 4-week negative control groups of SHR and WKY rats (n=8 per group) receive vehicle while positive control groups (Val group， n=8 per group) receive valsartan (20 mg/kg/day) for comparison[4]. Mice: To study the in vivo anti-inflammatory efficacy of baicalein， graft-versus-host disease (GVHD) model is used. Splenic lymphocytes from C57BL/6 mice are incubated with baicalein in vitro (25 μM， 4h) and adoptively transferred to immune-compromised Balb/c mice[2]. |
| 数据来源文献 |
[1]. Shieh DE，et al. Antioxidant and free radical scavenging effects of baicalein， baicalin and wogonin. Anticancer Res. 2000 Sep-Oct;20(5A):2861-5.
[2]. Patwardhan RS， et al. Baicalein exhibits anti-inflammatory effects via inhibition of NF-κB transactivation. Biochem Pharmacol. 2016 May 15;108:75-89.
[3]. Ma X， et al. Baicalein suppresses metastasis of breast cancer cells by inhibiting EMT via downregulation of SATB1 and Wnt/β-catenin pathway.
Drug Des Devel Ther. 2016 Apr 18;10:1419-41.
[4]. Kong EK， et al. A novel anti-fibrotic agent， baicalein， for the treatment of myocardial fibrosis in spontaneously hypertensiverats. Eur J Pharmacol. 2011 May 11;658(2-3):175-81. |
| 规格 |
0.1ml 0.3ml 0.5ml 1ml 1.5ml |
| 单位 |
瓶 |
中文
英语
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