CAS |
No.331-39-5 |
中文名称 |
咖啡酸(10mM in DMSO,无菌) |
英文名称 |
Caffeic acid(10mM in DMSO,Sterile) |
分子式 |
C9H8O4 |
分子量 |
180.15 |
溶解性 |
请根据自己的实验要求使用。 |
外观(性状) |
无菌溶液 |
储存条件 |
Stroe at -20℃,6 months. |
靶点 |
TRP Channel |
通路 |
Membrane Transporter&Ion Channel;Neuronal Signaling |
背景说明 |
Caffeic acid 是 TRPV1 离子通道和 5-脂氧合酶 (5-LO) 的抑制剂。 |
生物活性 |
Caffeic acid is an inhibitor of both TRPV1 ion channel and 5-Lipoxygenase (5-LO).[1-2] |
In Vitro |
Caffeic acid 对组胺诱导的反应具有抑制作用,当用于预处理的浓度从 0.1 增加到 1 mM 时,Caffeic acid 的抑制作用逐渐增加,类似于典型的剂量依赖性反应。用 1 mM Caffeic acid 预处理 HEK293T-TRPV1 细胞可显著抑制辣椒素诱导的反应。当使用较低浓度的 Caffeic acid 时,辣椒素诱导反应的抑制作用不太明显。钙成像实验表明,Caffeic acid 孵育可显著抑制组胺敏感的背根神经节 (DRG) 神经元。用 Caffeic acid (1 mM) 预处理导致对组胺应用有反应的 DRG 神经元的百分比从 12.5% 显著降低到 2.1%。用 1 mM Caffeic acid 预处理可显著阻断 TRPA1 表达细胞中异硫氰酸烯丙酯 (AITC) 诱导的细胞内钙增加。Caffeic acid 还能够阻断 AITC 诱导的 TRPA1 激活[1]。 |
In Vivo |
用 Caffeic acid (500 mg/kg) 预处理的小鼠表现出明显较少的组胺诱导的抓挠 (30.50±10.87 次/1 小时,n=6)。进一步发现,较低剂量的 Caffeic acid (100 mg/kg) 在组胺引起的抓挠中的抗抓挠效果并不显著,尽管似乎有减少的趋势 (49.40±12.35 次/1 h,n=5) . 500 mg/kg Caffeic acid 预处理可显著抑制氯喹诱导的抓挠 (161.6±31.42 次/1 h,n=5)[1]。Caffeic acid 显著降低 5-LO mRNA (P[2]。 |
细胞实验 |
To determine cell viability, MTT assay is performed. HEK293T cells are cultured in 96-well plate at 37°C, a day before so that the confluence of cell is 85 to 90% on the actual day of the experiment. On the day of the experiment the cells are treated with different concentration of Caffeic acid for 10 min. Control cells are treated only with media. After removing supernatant and washing with PBS, MTT reagent (5 mg/mL) is added directly to fresh media. Cells are then incubated at 37°C for additional 4 h followed by draining of the media and overnight storage in dark condition. The next day, DMSO is added to each well and mixed in shaker for 10 min after which plate is read using microplate recorder at 490 nm with a reference wavelength of 620 nm. The relative cell viability (%) is expressed as a percentage relative to the untreated control cells[1]. |
动物实验 |
Rats are divided into five groups: the sham group (n=9), ischemia-reperfusion (I/R) non-treated group (n=9), I/R-Caffeic acid group (10 mg/kg) (n=9), I/R-Caffeic acid group (30 mg/kg) (n=9) and I/R- Caffeic acid group (50 mg/kg) (n=9). In I/R-Caffeic acid groups, the rats are administrated Caffeic acid at 10, 30, 50 mg/kg (prepared with 0.3% sodium carboxymethyl cellulose) by intraperitoneal injection at 30 min prior to ischemia. The sham group and I/R group are treated with an equal volume of 0.3% sodium carboxymethyl cellulose[2]. |
数据来源文献 |
[1]. Pradhananga S, et al. Caffeic acid exhibits anti-pruritic effects by inhibition of multiple itch transmission pathways in mice. Eur J Pharmacol. 2015 Sep 5;762:313-21. [2]. Liang G, et al. The protective effect of caffeic acid on global cerebral ischemia-reperfusion injury in rats. Behav Brain Funct. 2015 Apr 18;11:18. |
规格 |
1ml |
单位 |
瓶 |