| CAS |
No.4261-42-1 |
| 中文名称 |
异荭草苷(10mM in DMSO,无菌） |
| 英文名称 |
Luteolin-6-C-glucoside(10mM in DMSO，Sterile） |
| 分子式 |
C21H20O11 |
| 分子量 |
448.38 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
COX |
| 通路 |
Immunology & Inflammation |
| 背景说明 |
Isoorientin是一种有效的 COX-2 抑制剂。 |
| 生物活性 |
Isoorientin is a potent inhibitor of COX-2 with an IC50 value of 39 μM.[1-3] |
| In Vitro |
Isoorientin 是 Pueraria tuberosa 块茎中 Cyclooxygenase-2 (COX-2) 的选择性抑制剂[1]。PANC-1 和 PATU-8988 细胞在 Isoorientin (0、20、40、80 和 160 μM) 存在的情况下生长 24 小时，并添加 CCK8 溶液。在 20、40、80 和 160 μM 的浓度下，细胞活力显著降低。将细胞与 Isoorientin (PANC-1 为 0、20、40、80 和 160 μM；PATU-8988 为 0、20、40、80、160 和 320 μM) 培养 24 小时后，p-AMPK 的表达 和 AMPK 通过蛋白质印迹法进行评估。Isoorientin 处理后，p-AMPK 表达增加。然后在 shRNA 组中，以 80 μM 的浓度检测 Isoorientin 的作用。shRNA 组中 AMPK 和 p-AMPK 的表达水平远低于野生型 PC 细胞 (WT) 和转染阴性对照慢病毒 (NC) 组[2]。 |
| In Vivo |
用 10 mg/kg 和 20 mg/kg 体重的 Isoorientin 处理动物爪水肿在统计学上显著减少，平均峰值厚度分别为 1.19±0.05 毫米和 1.08±0.04 毫米。这表明与对照组相比，Isoorientin 可显著减轻足部水肿[3]。 |
| 细胞实验 |
PANC-1 and PATU-8988 cells are plated onto 96-well plates. Each well contain ~5，000 cells and 200 μL of the medium with 10% FBS. When the cells of each well reach 70% confluency， the medium is changed， and FBS-free medium with different concentrations of Isoorientin is added. After 24 hours， the cells are washed with PBS once， the medium containing Isoorientin is discarded， and 100 μL of FBS-free medium with 10 μL of the Cell Counting Kit 8 (CCK8) reagent are added. The cells are incubated for another 1-2 hours at 37°C， and the absorbance of each well is detected using an ELISA reader at 490 nm. Cell viability is expressed as the fold change of absorbance[2]. |
| 动物实验 |
Mice[3]In the case of paw edema model the Isoorientin or Celecoxib is given intraperitoneally and Carrageenan is injected into the paw directly one hour later. In air pouch model all the treatments are given along with Carrageenan directly into the pouch cavity. Isoorientin is injected three hours earlier than injection of Carrageenan into the pouch cavity. Isoorientin and Celecoxib are administered into the mice air pouch. The stock solutions of Isoorientin (100?mg/mL) and Celecoxib (100?mg/mL) are prepared in DMSO and further dilutions are made at the time of treatments. Animals are divided， into 5 different groups as follows: control (DMSO treated); Carrageenan (0.5?mL of 1.5% (w/v) Carrageenan in saline) treated; Carrageenan+Celecoxib (20?mg/kg body weight) treated; Carrageenan+Isoorientin (10?mg/Kg body weight) treated; Carrageenan+Isoorientin (20?mg/Kg body weight) treated. |
| 数据来源文献 |
[1]. Sumalatha M， et al. Isoorientin， a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa. Nat Prod Commun. 2015 Oct;10(10):1703-4.
[2]. Ye T， et al. Isoorientin induces apoptosis， decreases invasiveness， and downregulates VEGF secretion by activating AMPK signaling in pancreatic cancer cells. Onco Targets Ther. 2016 Dec 12;9:7481-7492.
[3]. Anilkumar K， et al. Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa. Oxid Med Cell Longev. 2017;2017:5498054. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
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