五味子甲素(10mM in DMSO,无菌）_索莱宝|主营生物科研试剂&抗体|蛋白|多肽|试剂盒|专业定制化服务平台-Solarbio【网上商城】

生化试剂

抗生素维生素动植物激素酸&盐&胺糖及衍生物脂质＆脂肪酸衍生物核酸&核苷酸及衍生物氨基酸&蛋白质酶&辅酶表面活性剂&去垢剂&螯合剂氘代试剂稳定同位素反应底物常规生化试剂通用缓冲液

蛋白与免疫

重组蛋白蛋白提取/定量免疫佐剂细胞全蛋白抗原蛋白电泳抗体标记试剂盒其它实验试剂

多肽

封闭肽化妆品肽目录肽定制肽

抗体

一抗二抗内参抗体细菌抗体标签抗体 RNA抗体植物抗体小分子抗体病毒抗体

ELISA

人小鼠大鼠通用其他种属未包被试剂盒抗体对 Elisa辅助试剂

流式分析

流式辅助试剂

分子生物学

核酸提取/纯化 PCR/qPCR 分子克隆相关酶核酸电泳重组克隆表达质粒分子杂交分子生物学辅助试剂 MBI原装

细胞生物学

细胞系（株）细胞器提取试剂盒细胞培养基Kit 细胞培养与消化细胞分离液细胞生长因子细胞检测鲎试剂植物培养

小分子化合物

迷你化合物Kit 抑制剂＆拮抗剂＆激动剂疾病造模剂明星产品 Peptides 化合物库 PROTAC/蛋白降解靶向嵌合分子同位素标记物氨基酸及其衍生物 Anti-infection/抗感染糖类及其衍生物维生素及其衍生物助溶剂&增溶剂染料&探针反应底物&理化检测实验常用溶液

生化试剂盒

氮代谢系列脂肪酸代谢系列糖类代谢系列其他生化系列氧化还原系列蛋白及酶类系列能量代谢系列

染色试剂

染色原料荧光染色液 HE染色液组织染色液植物染色液酶类染色液特殊细胞染色液胞内组分染色液微生物染色液辅助试剂组织切片制备试剂

分析标准品

分析对照品紫外分光标准品可溯源标准品对照药材仪器检定标液单标溶液混标套装液相色谱用试剂盒分析辅助试剂质控标样/基质标物代销产品

微生物培养

菌株培养基基础培养基原料培养基添加剂发酵专用产品酵母缺陷型培养基其他微生物培养基微生物辅助试剂 OXOID原装

层析介质

预装柱填料

磁珠

纯化磁珠免疫沉淀纳米抗体磁珠基础磁珠

仪器、耗材

基础耗材培养耗材 PCR&分子生物学耗材 WB&免疫耗材药敏纸片病理制片耗材安全防护耗材其他耗材层析用耗材实验仪器

纳米材料

石墨/石墨烯类石墨烯碳纳米材料无机纳米材料分子筛金属纳米材料其它纳米材料

化学合成

分子砌块 PEG衍生物金属材料

SKU	北京总部	北京海淀	武汉	广州
IS00501-1ml	咨询	咨询	咨询	咨询

CAS	No.61281-38-7
中文名称	五味子甲素(10mM in DMSO,无菌）
英文名称	Schisandrin A(10mM in DMSO，Sterile）
分子式	C₂₄H₃₂O₆
分子量	416.51
溶解性	请根据自己的实验要求使用。
外观(性状)	无菌溶液
储存条件	Stroe at -20℃，6 months.
靶点	Adiponectin receptor 2;Cytochrome P450
通路	GPCR & G Protein;Metabolic Enzyme&Protease
背景说明	是五味子的活性成分，具有保肝、抗肿瘤和抗氧化活性。也是脂联素受体 2 (AdipoR2) 的激动剂，可以抑制 CYP3A 活性。
生物活性	Schisandrin A inhibits CYP3A activity with an IC50 of 6.60 μM and Ki of 5.83 μM， respectively.[1-2]
In Vitro	Schisandrin A (Sch A) strongly inhibits microsomal midazolam 1-hydroxylation catalyzed by CYP3A， with an IC50 of 6.60 μM. The recovery of enzyme activity in the absence or presence of Schisandrin A is shown in dilution assay plots. The Ki value for Schisandrin A is obtained from the Dixon plots and is 5.83 μM. The inactivation of rat liver microsomal midazolam 1-hydroxylation activity by Schisandrin A in the presence of NADPH is found to be time- and concentration-dependent. The Kinact and Ki are estimated to be 0.134/min and 4.51 μM， respectively for Schisandrin A[1].
In Vivo	Schisandrin A (SchA) significantly inhibits CYP3A activity in rat hepatic microsomes and Vmax value of each group in a concentration-dependent manner. The double-reciprocal plots and the secondary plot show that Schisandrin A inhibits CYP3A activity， with an apparent Ki value of 30.67 mg/kg. In each Schisandrin A-treated group， Schisandrin A also significantly decreases 1-hydroxymidazolam plasma concentrations compared with the negative group (to levels similar to the positive group)[2].
动物实验	Rats[2]Healthy male Sprague-Dawley rats， weighing 250-280 g and 2-3 months of age， are used. The rats are randomly divided into five groups with 16 rats in each group. The animals are administered once daily for three consecutive days. The Schisandrin A-treated groups are administered intragastrically with doses of 32， 16 or 8 mg/kg of Schisandrin A (physiological saline as vehicle)， and the rats are similarly administered with equal volume of vehicle in the negative control group and Ketoconazole (75 mg/kg) in the positive control group. All animals are allowed free access to food but are fasted overnight before scarification to reduce the intestinal content， and each group is randomly divided into two parts with eight rats in each part[2].
激酶实验	For the inactivation of CYP3A4 activity， microsomes are preincubated with inhibitors (Schisandrin A， 2.4 μM， 7.2 μM and 12.0 μM; or Sch B) at 37°C for up to 15 min in the presence of NADPH. Reactions are initiated with the addition of substrate midazolam and incubated at 37°C for 10 min. The enzyme inactivation is analyzed. Duplicates are prepared and tested[1].
数据来源文献	[1]. Li WL， et al. Inhibitory effects of Schisandrin A and Schisandrin B on CYP3A activity. Methods Find Exp Clin Pharmacol. 2010 Apr;32(3):163-9. [2]. Li WL， et al. Inhibitory effects of continuous ingestion of Schisandrin A on CYP3A in the rat. Basic Clin Pharmacol Toxicol. 2012 Feb;110(2):187-92.
规格	1ml
单位	瓶