| CAS |
No.520-18-3 |
| 中文名称 |
山奈酚(10mM in DMSO,无菌） |
| 英文名称 |
Kaempferol(10mM in DMSO，Sterile） |
| 分子式 |
C15H10O6 |
| 分子量 |
286.24 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Estrogen Receptor |
| 通路 |
Endocrinology & Hormones |
| 背景说明 |
Kaempferol是一种黄酮，是ERRα和ERRγ的反向激动剂，可用于乳腺癌研究。 |
| 生物活性 |
Kaempferol (Kempferol)， a flavonoid found in many edible plants， inhibits estrogen receptor α expression in breast cancer cells and induces apoptosis in glioblastoma cells and lung cancer cells by activation of MEK-MAPK. Kaempferol can be uesd for the research of breast cancer.[1-4] |
| In Vitro |
Kaempferol 还通过抑制 Src 激酶和下调 NFκB 通路来抑制白细胞介素 4 和环加氧酶 2 的表达，从而具有抗炎作用。山奈酚还可有效抑制卵巢癌细胞的血管生成和诱导细胞凋亡[1]。Kaempferol 是一种天然黄酮类化合物，广泛分布于水果和蔬菜中，前瞻性研究表明，几十年来，食用 Kaempferol 可显著降低美国女护士患卵巢癌的风险。经过 24 小时的处理后，Kaempferol 对所有 3 种受试卵巢癌细胞的增殖产生显著的浓度依赖性抑制。在 40 μM 或更高浓度的处理时观察到这种抑制作用[2]。Kaempferol 是一种类黄酮，广泛存在于多种植物衍生食品和传统药物中使用的叶子中。Kaempferol 显著抑制 NADPH 氧化酶活性。山奈酚通过直接结合 NADPH 氧化酶来减少活性氧 (ROS)。Kaempferol 通过降低 CAMKII 氧化来防止 Ang II 诱导的窦房结细胞死亡[3]。10 -20 μM Kaempferol 剂量依赖性地抑制其在致敏 RBL-2H3 细胞中的释放。当将 10 -20 μM Kaempferol 补充到 DNP-BSA 攻击的 RBL-2H3 细胞中 15 分钟时，Syk 和 PLCγ 的激活被高度减弱。当向 DNP-BSA 攻击的 RBL-2H3 细胞中加入 ≥10 μM Kaempferol 60 分钟时，COX2 诱导减少[4]。 |
| In Vivo |
COX2 诱导在 BSA 攻击的 BALB/c 小鼠的气道中得到证实。观察到未处理的对照小鼠的气道中缺乏 COX2。小鼠吸入 BSA 导致小鼠气道中 COX2 诱导增强 (深棕色染色)，这可通过口服山奈酚逆转。在 BSA 攻击的小鼠中，观察到明显的杯状细胞增生和上皮增厚。当 BSA 攻击小鼠补充 20 mg/kg Kaempferol 时，上皮增厚完全消失[4]。 |
| 细胞实验 |
Ovarian cancer cells are seeded in 96-well plates at 2000 cells/well and incubated overnight before treatment with 0-160 μM Kaempferol for 24 hours in triplicates. The medium is removed， and the plates are freeze-thawed to lyse cells. Each well is added with 200 μL 1× CyQUANT cell lysis buffer containing 5x SYBR Green I and incubated at room temperature (RT) for 5 minutes. The reaction (50 μL) is transferred to PCR strip tubes and the fluorescent signal is measured at 90°C with a real-time Chromo4 PCR instrument. To ensure that cell proliferation assays are performed within a linear range of cell numbers， a standard curve is generated by seeding different amount of OVCAR-3 cells (based on counting with a hemacytometer) in a 96-well plate， and measuring genomic DNA abundance after overnight incubation. Three independent experiments are performed and data is pooled for statistical analysis[2]. |
| 动物实验 |
Mice[4]Three-week-old male BALB/c mice are randomly assigned to the four treatment groups as follows (n=8 per group). (1) PBS-sensitized mice; (2) BSA-sensitized mice; (3) BSA-sensitized and 10 mg/kg Kaempferol-administered mice; and (4) BSA-sensitized and 20 mg/kg Kaempferol-administered mice. Mice are given a commercial mouse chow diet containing 20.5% protein， 3.5% fat， 8% fiber， 8% ash， and 0.5% phosphorus and are allowed access to food and water ad libitum. The mice are kept under a 12 h light and dark cycle at 23±1°C with 50%±5% relative humidity in specific pathogen-free conditions. Mice are allowed to become accustomed to their surroundings for one week before starting the allergic experiments. Sensitization of all experimental mice is carried out by subcutaneous injection with 20 μg BSA in 30 μL PBS and 50 μL Imject Alum on days 0 and 14. The control mice are injected with a combination of 50 μL PBS and 50 μL Imject Alum without BSA. On days 28， 29， and 30， only the experimental mice sensitized to BSA are subject to inhalation of 5% BSA， while control mice are challenged with 5% PBS for 20 min in a plastic chamber connected to a Medel aerosol nebulizer. All mice are sacrificed 24 h after the last challenge. Whole blood samples are directly used to measure the contents of eosinophils， basophils and neutrophils. The right lung is stored in 4% paraformaldehyde until use. |
| 激酶实验 |
Right atria or sinus nodal cells are homogenized in lysis buffer consisting of (50 mM Tris-HCl pH 7.5， 100 mM KCl， 1 mM ethylenediamine tetraacetic acid， 1 mM ethylene glycol tetraacetic acid， 1 mM dithiothreitol， 0.1 mM phenylmethylsulfonyl fluoride， 0.5 mM Benzamidine， 20 mg/L Leupeptin， 20 mM sodium pyrophosphate， 50 mM NaF， and 50 mM sodium β-glycerophosphate)， and total protein content is determined by the Bradford assay. Caspase-3 activity is determined by EnzChek Caspase-3 Assay Kit[3]. |
| 数据来源文献 |
[1]. Luo H， et al. Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability. Int J Nanomedicine. 2012; 7: 3951-3959.
[2]. Luo H， et al. Kaempferol induces apoptosis in ovarian cancer cells through activating p53 in the intrinsic pathway. Food Chem. 2011 September 15; 128(2): 513-519.
[3]. An M， et al. Protective effects of Kaempferol against cardiac sinus node dysfunction via CaMKII deoxidization. Anat Cell Biol. 2015 Dec;48(4):235-43.
[4]. Shin D， et al. Dietary Compound Kaempferol Inhibits Airway Thickening Induced by Allergic Reaction in a Bovine Serum Albumin-Induced Model of Asthma. Int J Mol Sci. 2015 Dec 16;16(12):29980-95. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
说明书下载
京公网安备 11011202000391号