| CAS |
No.4205-91-8 |
| 中文名称 |
盐酸可乐定(10mM in DMSO,无菌） |
| 英文名称 |
Clonidine Hydrochloride(10mM in DMSO，Sterile） |
| 分子式 |
C9H9Cl2N3·HCl |
| 分子量 |
266.55 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 运输条件 |
冷冻运输 |
| 靶点 |
Adrenergic Receptor |
| 通路 |
Endocrinology & Hormones;GPCR & G Protein;Neuronal Signaling |
| 背景说明 |
Clonidine hydrochloride 是 α2-adrenoceptor 激动剂。 |
| 生物活性 |
Clonidine hydrochloride is an agonist of α2-adrenoceptor and potent antihypertensive agent.[1-4] |
| In Vitro |
Clonidine (0.01， 0.1 or 1 μM) significantly induces CGRP (α and β) mRNA expression in a dose-dependent manner in endothelial cells. Clonidine treatment (1 μM) for 24 h significantly increases the NO level in endothelial cells. NO pathway modulates CGRP production induced by clonidine[2]. |
| In Vivo |
Clonidine (50 μg/kg， i.p.) induces a significant decrease in body temperature of rat lasting 3 hr， with the maximum at 1 hr after administration. An intracerebroventricular pretreatment of rats with neutral doses of phentolamine 15 min before clonidine considerably antagonizes the clonidine-induced hypothermia[1]. Clonidine (0.003-0.05 mg/kg， i.p.) potently suppresses dopamine efflux in the prefrontal cortex induced by PCP. Pretreatment with the alpha-2A receptor antagonist (BRL-44408) prevents clonidine from suppressing PCP-induced dopamine overflow in the prefrontal cortex[3]. In DMSO-pretreated SO rats， clonidine (0.6 μg i.c.) has no effect on blood pressure. However， after central adenosine A1R blockade (DPCPX) in SO rats， clonidine significantly (P 0.05， one-way ANOVA) clonidine-evoked reduction in blood pressure in ABD rats. In DPCPX-pretreated SO rats and along with the appearance of the hypotensive response， clonidine causes a significant (P [4]. |
| 动物实验 |
On the day of the experiment， the flow rate is increased to 2 μL/min approximately 2 h before beginning the collection of baseline samples. Dialysates are collected every 20 min; after 4 baseline samples are collected， animals are pretreated with an intra-peritoneal (i.p.) injection of either 0.9% saline (the vehicle)， clonidine (0.0033， 0.01 or 0.05 mg/kg) or guanfacine (0.05 or 0.5 mg/kg)， before receiving an injection of PCP (2.5 mg/kg， i.p.) 20 min later. In a separate study， BRL (1.0 mg/kg) is administered 20 min prior to clonidine. In addition， for some control experiments， the animals only receive one injection of saline， clonidine (0.01 or 0.05 mg/kg)， guanfacine (0.5 mg/kg) or BRL (1.0 mg/kg). |
| 数据来源文献 |
[1]. Bugajski J， et al. The involvement of central alpha-adrenergic and histamine H2-receptors in the hypothermia induced by clonidine in the rat. Neuropharmacology. 1980 Jan;19(1):9-15.
[2]. Nassar N， et al. Brainstem adenosine A1 receptor signaling masks phosphorylated extracellular signal-regulated kinase 1/2-dependent hypotensive action of clonidine in conscious normotensive rats. J Pharmacol Exp Ther. 2009 Jan;328(1):83-9.
[3]. Zhang YM， et al. Clonidine induces calcitonin gene-related peptide expression via nitric oxide pathway in endothelial cells. Peptides. 2009 Sep;30(9):1746-52.
[4]. Jentsch JD， et al. Clonidine and guanfacine attenuate phencyclidine-induced dopamine overflow in rat prefrontal cortex: mediating influence of the alpha-2A adrenoceptor subtype. Brain Res. 2008 Dec 30;1246:41-6. |
| 规格 |
0.1ml 0.3ml 0.5ml 1ml 1.5ml |
| 单位 |
瓶 |
中文
英语
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