| CAS |
No.472-15-1 |
| 中文名称 |
白桦脂酸(10mM in DMSO,无菌） |
| 英文名称 |
Betulinic acid(10mM in DMSO，Sterile） |
| 分子式 |
C30H48O3 |
| 分子量 |
456.7 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Topoisomerase |
| 通路 |
DNA Damage/DNA Repair |
| 背景说明 |
Betulinic acid 是一种真核细胞拓扑异构酶 I (topoisomerase I) 的抑制剂。 |
| 生物活性 |
Betulinic acid is a natural pentacyclic triterpenoid， acts as a eukaryotic topoisomerase I inhibitor， with an IC50 of 5 μM， and possesses anti-HIV， anti-malarial， anti-inflammatory and anti-tumor properties.[1-5] |
| In Vitro |
Betulinic acid is a eukaryotic topoisomerase I inhibitor， with an IC50 of 5 μM， and prevents topoisomerase I-DNA interaction[1]. Betulinic acid (10， 20， 40， 80， and 160 μM) significantly suppresses MDA-MB-231 cell viability in a time- and dose-dependent manner after treatment for 24 or 48 h.? Betulinic acid (20， 40 μM) causes decrease in Bcl-2 expression of MDA-MB-231 cells. Betulinic acid also induces morphological changes of MDA-MB-231 cells at 20 μM， and leads to ultrastructure changes of MDA-MB-231 cells at 40 μM[2]. Betulinic acid shows anti-HIV activities， with an EC50 of 1.4 μM in acutely infected H9 lymphocytes[4]. |
| In Vivo |
Betulinic acid (10 and 30 mg/kg， p.o.) significantly abrogates colon shortening， and reduces malondialdehyde (MDA) and lipid hydroperoxide levels in dextran sulfate sodium (DSS)-induced colitis in mice. Betulinic acid (30 mg/kg， p.o.) restores superoxide dismutase (SOD)， catalase activity and glutathione (GSH) content to control levels in DSS-induced colitis in mice. Betulinic acid (30 mg/kg， p.o.) also inhibits the DSS-induced increase in inflammatory markers. Betulinic acid (3， 10， 30 mg/kg， p.o.) suppresses acetic acid-induced writhing responses and mustard oil (MO)-induced visceral nociception in mice[3]. |
| 细胞实验 |
CCK-8 is used in the assay. MDA-MB-231 cells are cultured in 96-well plates at a density of 2 × 103 cells/well and then treated with DMSO vehicle or various concentrations of Betulinic acid ranging from 5 μM to 160 μM in 100 μL of medium for the indicated times. After the treatment period， the medium in each well is replaced with 110 μL of medium containing 10 μL of the CCK-8 mixture， and the plates are incubated for 1 h and 30 min at 37°C. The absorbance at a wavelength of 450 nm is measured with a microplate reader[2]. |
| 动物实验 |
Female Swiss albino mice are administered vehicle (5% v/v DMSO in peanut oil) or Betulinic acid (3， 10 or 30 mg/kg) in vehicle， orally. After 1 h， acetic acid (300 mg/kg) is administered by intraperitoneal route and number of writhing response of each animal is counted for 20 min by an observer who is blind to the treatments. Writhing response is when animal rubs its abdomen on surface of table/floor with elongation of the body and extension of the hind limbs[3]. |
| 数据来源文献 |
[1]. Chowdhury AR， et al. Betulinic acid， a potent inhibitor of eukaryotic topoisomerase I: identification of the inhibitory step， the major functional group responsible and development of more potent derivatives. Med Sci Monit. 2002 Jul;8(7):BR254-65.
[2]. Gao Y， et al. Betulinic acid induces apoptosis and ultrastructural changes in MDA-MB-231 breast cancer cells. Ultrastruct Pathol. 2018 Jan-Feb;42(1):49-54.
[3]. Kalra J， et al. Betulinic acid alleviates dextran sulfate sodium-induced colitis and visceral pain in mice. Naunyn Schmiedebergs Arch Pharmacol. 2017 Dec 26.
[4]. Hashimoto F， et al. Anti-AIDS agents--XXVII. Synthesis and anti-HIV activity of betulinic acid and dihydrobetulinic acid derivatives. Bioorg Med Chem. 1997 Dec;5(12):2133-43.
[5]. Kasperczyk H， et al. Betulinic acid as new activator of NF-kappaB: molecular mechanisms and implications for cancer therapy. Oncogene. 2005 Oct 20;24(46):6945-56. |
| 规格 |
1ml |
| 单位 |
瓶 |
中文
英语
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