| CAS |
No.59-14-3 |
| 中文名称 |
5-溴-2'-脱氧尿苷(10mM in DMSO,无菌） |
| 英文名称 |
5-BrdU(10mM in DMSO，Sterile） |
| 分子式 |
C9H11BrN2O5 |
| 分子量 |
307.1 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
DNA/RNA Synthesis |
| 通路 |
Cell Cycle;DNA Damage/DNA Repair |
| 背景说明 |
5-BrdU是一种核苷类似物，可与胸苷竞争掺入DNA。 |
| 生物活性 |
5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.[1-2] |
| In Vitro |
Bromodeoxyuridine 诱导癌细胞系和癌症干细胞群扩增 RG2 大鼠神经胶质瘤细胞的进行性、剂量反应性抑制。在 H9 细胞和 BJ 成纤维细胞中，溴脱氧尿苷改变细胞周期特征[1]。BrdU 稳定地整合到 DNA 中，因此可用于评估细胞增殖和其他细胞过程[2]。 |
| In Vivo |
Bromodeoxyuridine (300 mg/kg，腹腔注射或 0.8 mg/mL，口服) 显著减缓大鼠神经胶质瘤 RG2 肿瘤模型的肿瘤进展[1]。 |
| 细胞实验 |
Cultures are initially plated at 2000 cells/cm2?and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0， 1， 10， or 50 μM BrdU for 24 hours， and cumulative growth curves are obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5， 12， and 18 after treatment. |
| 数据来源文献 |
[1]. Levkoff LH， et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16.
[2]. Rothaeusler K， et al. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr;Chapter 7:Unit7.31 |
| 规格 |
0.1ml 0.3ml 0.5ml 1ml 1.5ml |
| 单位 |
瓶 |
中文
英语
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