| CAS |
No.446-72-0 |
| 中文名称 |
染料木素(10mM in DMSO,无菌） |
| 英文名称 |
Genistein(10mM in DMSO，Sterile） |
| 分子式 |
C15H10O5 |
| 分子量 |
270.24 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
EGFR |
| 通路 |
Angiogenesis;Protein Tyrosine Kinase/RTK |
| 背景说明 |
Genistein是一种大豆异黄酮，还是一种多重的酪氨酸激酶抑制剂。Genistein具有诱发细胞程序性死亡、改变细胞周期和血管生成以及抑制转移等活性，可用于癌症化学预防剂的相关研究。 |
| 生物活性 |
Genistein， a soy isoflavone， is a multiple tyrosine kinases (e.g.， EGFR) inhibitor which acts as a chemotherapeutic agent against different types of cancer， mainly by altering apoptosis， the cell cycle， and angiogenesis and inhibiting metastasis.[1-2] |
| In Vitro |
Genistein 抑制血清刺激的 MCF-7 和 T47D ER+ 细胞生长，染料排斥的 IC50 值分别为 7.6 和 8.7 μg/mL，以及 8.7 和 10.6 μg/mL 分别由[3H]胸腺嘧啶掺入。这些值类似于通过 MTT 测定获得的 MCF-7 和 T47D ER+ 细胞的 IC50 值，分别为 9.4 和 7 μg/mL。此外，在 8 小时的孵育期内，与对照细胞相比，浓度高达 20 μg/mL 的 Genistein 不会改变 MTT 线粒体减少。此外，在 IC50 浓度下，Biochanin A 或大豆苷元均未发现干扰 MTT 测定。因此，MTT 法可有效确定所研究系统中 Genistein 在浓度低于 20 μg/mL 时的生长抑制作用[1]。 |
| In Vivo |
Bisphenol A (BPA) 单独处理和联合 Genistein 对 STD 或 HFD 喂养大鼠肝脏中 LC3II 和 PPARα 的蛋白表达无显著影响 (P>0.05；P>0.05)。与 HFD 组或 HFD-BPA 组相比，观察到在大鼠中添加 Genistein 后肝脏中 PPARγ 的蛋白表达显著降低[2]。 |
| 细胞实验 |
The IC50 values for Genistein are determined by the MTT assay. Briefly， the MTT assay is a colorimetric assay that is based on the ability of living but not dead cells to reduce a tetrazolium-based compound to a blue formazan product. The formazan crystals are solubilized in DMSO， and the absorbance is measured at 540 nm. The absorbance at 540 nm is proportional to the number of viable cells. The lC50 values obtained with the MTT assay are compared with the lC50 values obtained by counting viable cells using trypan blue dye exclusion and by tritiated thymidine incorporation into DNA[1]. |
| 动物实验 |
Mice[2] Balb/c male mice are used. Genistein is administered as follows: On days 1-30， Genistein once daily， interaperitoneally injecting. Morphine plus Genistein is administered as follows: On days 1-30， Genistein once daily plus morphine， interaperitoneally injecting (17， 18). The same volume of saline is administered. Mice are randomly divided into 8 groups (n=6). 1) Normal saline group (1 mL DW/daily); 2) Morphine treated group; 3) Genistein 1 mg/kg treated group; 4) Genistein 2 mg/kg treated group 5) Genistein 4 mg/kg treated group; 6) Morphine plus Genistein 1 mg/kg treated group; 7) Morphine plus Genistein 2 mg/kg treated group; 8) Morphine plus Genistein 4 mg/kg treated group. Rats[3] Male 8-week-old Wistar rats (150-180g) are used. After one week acclimation， all rats are randomly divided into 8 groups with 10 rats per group and treated for 35 weeks as follows: (1) STD group is fed with rodent standard chow diet (STD); (2) STD-BPA group is fed with STD and administered with BPA (50 μg/kg/day); (3) STD-(BPA+G) group is fed with STD and administered with BPA (50 μg/kg/day) plus Genistein (10 mg/kg/day); (4) STD-G group is fed with STD and administered with Genistein (10 mg/kg/day); (5) HFD group received high-fat diet (HFD); (6) HFD-BPA group is fed with HFD and administered with BPA (50 μg/kg/day); (7) STD-(BPA+G) group is fed with HFD and administered with BPA (50 μg/kg/day) plus Genistein (10 mg/kg/day); (8) HFD-G group is fed with HFD and administrated with Genistein (10 mg/kg/day). All the male genitors are treated for 35 weeks consecutively. The details of BPA (50 μg/kg/day) and Genistein (10 mg/kg/day) treatment methods have been described previously: BPA is dissolved in corn oil and diluted with three stock solutions (20， 40， 80， and 120 μg/mL). |
| 数据来源文献 |
[1]. Peterson G， et al. Genistein inhibits both estrogen and growth factor-stimulated proliferation of human breast cancer cells. Cell Growth Differ. 1996 Oct;7(10):1345-51.
[2]. Ding S， et al. Environmentally Relevant Dose of Bisphenol A Does Not Affect Lipid Metabolism and Has No Synergetic or Antagonistic Effects on Genistein‘s Beneficial Roles on Lipid Metabolism. PLoS One. 2016 May 12;11(5):e0155352. |
| 规格 |
0.1ml 0.3ml 0.5ml 1ml 1.5ml |
| 单位 |
瓶 |
中文
英语
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