| CAS |
No.83905-01-5 |
| 中文名称 |
阿奇霉素(10mM in DMSO,无菌） |
| 英文名称 |
Azithromycin(10mM in DMSO，Sterile） |
| 分子式 |
C38H72N2O12 |
| 分子量 |
748.98 |
| 溶解性 |
请根据自己的实验要求使用。 |
| 外观(性状) |
无菌溶液 |
| 储存条件 |
Stroe at -20℃，6 months. |
| 靶点 |
Bacterial |
| 通路 |
Anti-infection |
| 背景说明 |
Azithromycin是一种大环内酯抗生素。 |
| 生物活性 |
Azithromycin is a macrolide antibiotic useful for the treatment of a number of bacterial infections.[1-2] |
| In Vitro |
Azithromycin (2 μM) augments rhinovirus-induced IFNβ expression in primary bronchial epithelial cells from asthmatics， which is associated with over-expression of RIG-I like receptors and repression of viral replication. Knockdown of MDA5， but not knockdown of RIG-I， diminishes azithromycin (2 μM)-enhanced viral-induced IFNβ expression in asthmatic primary bronchial epithelial cells[1]. Azithromycin specifically reduces MMP-9 mRNA and protein levels without affecting NF-κB in endotoxin-challenged monocytic THP-1 cells[2]. |
| In Vivo |
Azithromycin (50 mg/kg) has no effect on bronchoalveolar lavage inflammatory parameters and LDH levels in a mouse model of asthma exacerbation. Azithromycin induces neither general inflammatory parameters nor LDH release in a mouse model of asthma exacerbation， and augments expression of interferon-stimulated genes and the pattern recognition receptor MDA5 but not RIG-I in exacerbating mice[1]. |
| 细胞实验 |
THP-1 cells (106 cells in 1 mL RPMI medium， without antibiotics， growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next， 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm， 5 min). THP-1 cell viability is tested using 3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm. |
| 数据来源文献 |
[1]. Menzel M， et al. Azithromycin augments rhinovirus-induced IFNβ via cytosolic MDA5 in experimental models of asthma exacerbation. Oncotarget. 2017 Mar 18.
[2]. Vandooren J， et al. Differential inhibition of activity， activation and gene expression of MMP-9 in THP-1 cells by azithromycin and minocycline versus bortezomib: A comparative study. PLoS One. 2017 Apr 3;12(4):e0174853. |
| 规格 |
0.1ml 0.3ml 0.5ml 1ml 1.5ml |
| 单位 |
瓶 |
中文
英语
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