PACAP (1-27)，human，ovine，rat_索莱宝|主营生物科研试剂&抗体|蛋白|多肽|试剂盒|专业定制化服务平台-Solarbio【网上商城】

产品类型	多肽
CAS	No.127317-03-7
单字母序列	HSDGIFTDSYSRYRKQMAVKKYLAAVL-NH2
三字母序列	His-Ser-Asp-Gly-Ile-Phe-Thr-Asp-Ser-Tyr-Ser-Arg-Tyr-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Ala-Ala-Val-Leu-NH2
别名	PACAP 1-27
分子式	C142H224N40O39S1
分子量	3147.66
纯度	≥95%
外观(性状)	冻干粉;Lyophilized powder
溶解性	DMSO：100 mg/mL; *超声助溶;
盐体系	TFA盐;Trifluoroacetate salt
来源	合成;Synthetic
储存条件	Store at -20℃，2 years.(Avoid freeze/thaw cycles)
背景说明	PACAP (1-27)， human， ovine， rat (PACAP 1-27) 是 PACAP-38 的 N-末端片段，是一种有效的 PACAP 受体拮抗剂，对大鼠 PAC1，大鼠 VPAC1 和人 VPAC2 的 IC50 值分别为 3 nM，2 nM 和 5 nM。
In Vivo	The inhibitory effect of pituitary adenylate cyclase activating polypeptide (PACAP (1-27)， human， ovine， rat) on the increase in total pulmonary resistance (RL) causes either by allergen or histamine in anaesthetized， ventilated guinea-pigs is studied. PACAP (1-27)， human， ovine， rat given via i.v. infusion (0.045-4.5 nmol/kg/min) dose-dependently reduces the increase in RL caused by inhaled ovalbumin and histamine. At the highest dose， PACAP (1-27)， human， ovine， rat prevented the increase in RL caused by ovalbumin and histamine completely. Infusion of PACAP (1-27)， human， ovine， rat and the β2-adrenoceptor agonist， salbutamol (0.045-4.5 nmol/kg/min) inhibit the increase in RL similarly， but salbutamol increases the heart rate more than PACAP (1-27)， human， ovine， rat.
In Vitro	Radioligand receptor binding assays with I-monoiodinated PACAP (1-27)， human， ovine， rat confirms the presence of PAC -receptors on AR4-2J cells， since PACAP (1-27)， human， ovine， rat and PACAP(1–38) equipotently displaces radioligand binding with a Kd of 1-2 nM， whereas vasoactive intestinal peptide (VIP) is 1000-fold less potent. PACAP (1-27)， human， ovine， rat exhibits a distinct and much higher susceptibility to VIP-amino acid substitutions. PACAP (1-27)， human， ovine， rat has potency and binding affinity to stimulate IP3 and cAMP formation in AR4-2J cells.
参考文献	[1]. Gourlet P， et al. Fragments of pituitary adenylate cyclase activating polypeptide discriminate between type I and II recombinant receptors. Eur J Pharmacol. 1995 Dec 4;287(1):7-11. [2]. Sch fer H， et al. Structural motifs of pituitary adenylate cyclase-activating polypeptide (PACAP) defining PAC1-receptor selectivity. Regul Pept. 1999 Feb 5;79(2-3):83-92. [3]. Lindén A， et al. Inhibition of bronchoconstriction by pituitary adenylate cyclase activating polypeptide (PACAP 1-27) in guinea-pigs in vivo. Br J Pharmacol. 1995 Jul;115(6):913-6.
研究领域	神经科学研究
规格	1mg 5mg 10mg
单位	瓶
分类属性	目录肽