| 英文名称 |
Anti-Phospho-NF-κB p65 (Ser468) Monoclonal Antibody |
| 中文名称 |
磷酸化细胞核因子NF-κB p65抗体 |
| 宿主 |
Rabbit |
| 克隆类型 |
Monoclonal Antibody |
| 亚型 |
IgG |
| 稀释比例 |
WB 1:2000-10000. |
| 靶标 |
Phospho-NF-κB p65 (Ser468) |
| 别名 |
NF kB P65,NF-kB p65,NF-kB-p65,NFKBp65,NF-κBp65,NF-kBp65,Avian reticuloendotheliosis viral (v rel) oncogene homolog A,MGC131774,NFKB 3,NFKB3,RELA |
| 背景说明 |
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. |
| Swiss Prot |
Q04206 |
| Gene ID |
5970 |
| 亚细胞定位 |
Cytoplasm Nucleus |
| 免疫原 |
A synthetic peptide of human Phospho-NF-κB p65 (Ser468) |
| 理论分子量 |
65kDa |
| 实际分子量 |
75kDa |
| 纯化方法 |
Affinity purification |
| 储存液 |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 |
Store at -20℃. Avoid freeze / thaw cycles. |
| 规格 |
50ul 100ul |
| 交叉反应 |
Human Mouse |
| 应用 |
WB ELISA |
| 研究领域 |
免疫学 新陈代谢 细胞生物学 发育生物学 信号传导 |
| 单位 |
支 |
| 属性分类 |
修饰抗体 |
中文
英语
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