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番茄红素

CAS:No.502-65-8

英文名称:Lycopene

纯度:HPLC≥96%

分子式:C40H56

分子量:536.87

储存条件:Store at -20℃,1 year.

番茄红素
标准品同系列5送2
货号:
SL8700
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
SL8700-20mg 咨询 咨询 咨询 咨询
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说明:该产品极易被氧化,如发现产品变色,请停止使用或重新标定后使用,溶液状态可在-20℃条件下保存20h。

本品为分析标准品。

使用本产品的案例(仅供参考

UPLC

Chromatography was carried out with a Ultra-high performance liquid chromatography. Samples were measured by a C18 carotenoid column (100 mm × 2.1 i.d., 1.7 μm). The eluent was methyl tertiary butyl ether and methanol (V/V = 30:70) with the type of isocratic elution. And each eluent contained 0.01% BHT and 0.05% TEA (triethylamine) as modifiers in order to prevent the degradation of carotenoids on the column. The following rate was 0.5 mL/min, column temperature was 25 °C, and the injection volume was 5 μL, analyzing with UV at 445 nm.

来源文献:Shen YH, Yang FY, Lu BG, Zhao WW, Jiang T, Feng L, Chen XJ, Ming R. Exploring the differential mechanisms of carotenoid biosynthesis in the yellow peel and red flesh of papaya. BMC Genomics. 2019 Jan 16;20(1):49. doi: 10.1186/s12864-018-5388-0. PMID: 30651061; PMCID: PMC6335806.


LC-MS

The carotenoids contents were determined using an HPLC system equipped with a  C30 column (150 ×4.6 mm, 3 μm) and a UV/VIS detector. The mobile phase consisted ofA (acetonitrile: methanol = 3:1) and B (methyl tert-butyl ether), and a gradient elution program (Lee et al., 2001) was used. The flow rate was 1.0 ml/min, and the column temperature was 40℃. The signals were detected at 450 nm. Identification of lutein and zeaxanthin was conducted on AB TripleTOF 5600 plus system equipped with an APCI source operated in the positive ionization mode. The column and temperature settings were the same as HPLC. The mobile phase was A (methanol) and B (acetonitrile: isopropanol = 1:1) with linear increase of B from 0 to 100% in 30 min followed by dropping to 0 in 10 min. The source voltage was +5.5 kV, and the source temperature was 500℃. The pressure of Gas 1 (Air) and Gas 2 (Air) was set to 50 psi. The pressure of Curtain Gas (N2) was set to 35 psi. The m/z scan range of production was set as 50–1500. 

来源文献:Bian Q, Zhou P, Yao Z, Li M, Yu H, Ye L. Heterologous biosynthesis of lutein in S. cerevisiae enabled by temporospatial pathway control. Metab Eng. 2021 Sep;67:19-28. doi: 10.1016/j.ymben.2021.05.008. Epub 2021 May 30. PMID: 34077803.

备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

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