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CAS:No.223538-61-2
英文名称:(Z-DEVD)2-R110
分子式:C72H78N10O27
分子量:1515.46
储存条件:Powder:-20℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
纯度:≥95%
说明书下载
(Z-DEVD)2-R110是caspase-3/7的荧光酶底物，广泛用于高通量测定。多肽与罗丹明110 (R110)上的每个氨基共价连接，从而抑制染料的荧光。当肽被caspase切割时，底物首先转化为荧光单酰胺，然后转化为R110，荧光进一步增强。底物可用于使用荧光计或荧光微孔板读取器连续测量细胞提取物和纯化酶制剂中的酶活性。
Example protocol（仅供参考）
(Z-DEVD)2-R110 stock solution (10 mM)
Add 65 µL of DMSO into the vial of 1 mg (Z-DEVD)2-R110 to make 10 mM stock solution.
If it is necessary to prepare a stock solution, it is recommended to store in aliquots to avoid product failure caused by repeated freezing and thawing.
PREPARATION OF WORKING SOLUTION
Caspase 3/7 assay solution (2X):
Mix 50 µL (Z-DEVD)2-R110 stock solution (10 mM), 100 µL DTT (1M), 400 µL EDTA (100 mM) and 10 mL Tris Buffer (20 mM), pH =7.4.
SAMPLE EXPERIMENTAL PROTOCOL（仅供参考）
Mix equal volume of the caspase 3/7 standards or samples with 2X caspase 3/7 assay reaction solution and incubate at room temperature for at least 1 hour.
Monitor the fluorescence increase at Ex/Em = 490/525 nm.
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