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硫黄素T

CAS:No.2390-54-7

英文名称:Thioflavin T

分子式:C17H19ClN2S

分子量:318.86

储存条件:Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:≥98%

硫黄素T
满3999赠露营车
货号:
IT3780
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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当Thioflavin T与β片层结合时,例如淀粉样蛋白寡聚体中的那些,染料经历其激发光谱的特征性的120nm红移,其可以在450nm处被选择性激发,产生482nm的荧光信号。Thioflavin T快速且特异性结合由合成β-淀粉样蛋白(1-40)形成的反平行β-折叠原纤维,但不结合单体或寡聚中间体。Thioflavin T的结合不会干扰β-淀粉样肽聚集成淀粉样蛋白原纤维。该染料常用于可视化由阿尔茨海默病患者脑中发现的β-淀粉样蛋白组成的斑块。


使用本产品的案例(仅供参考

In Vitro

Aβ25–35 aggregation assay(20 μM Aβ25–35;20 μM Th-T solution

Th-T is a relatively common fluorescent dye for detecting Aβ aggregation.   The experiment was divided into three parts.   

①. Aβ25–35 aggregation assay: 20 μM of Aβ25–35 with and without baicalein (80 μM) were incubated at 37 °C for 6 days.   After incubation, 10 μl of the mixture was added into 190 μl of Th-T solution (20 μM) in a black 96-well plate.   The fluorescence intensity was measured daily at the same time for 6 consecutive days.   

②. Anti-aggregation assay of baicalein: A mixture of Aβ25–35 (20 μM) and baicalein (40, 60 and 80μM) was prepared in PBS, then the mixture was incubated at 37 °C for 6 days.   10 μl of the mixture was added into 190 μl of Th-T solution (20 μM), and the fluorescence intensity of the mixture was then measured.   

③. Disaggregation of Aβ25–35 amyloid fibrils by baicalein: Aβ25–35 (20 μM) solution was incubated alone for 3 days, then it was incubated with baicalein (40, 60 and 80 μM) for another 2 days at 37 °C.   After incubation 3 days or 5 days, 10 μl of the mixture was added into 190 μl of Th-T solution (20 μM), and the fluorescence intensity was measured using a microplate reader at 440 nm excitation and 490 nm emission.

来源文献:Gao L, Zhou F, Wang KX, Zhou YZ, Du GH, Qin XM. Baicalein protects PC12 cells from Aβ25-35-induced cytotoxicity via inhibition of apoptosis and metabolic disorders. Life Sci. 2020 May 1;248:117471. doi: 10.1016/j.lfs.2020.117471. Epub 2020 Feb 26. PMID: 32112868.




备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
Lv Z , Li L , Man Z ,et al.Polarity-activated super-resolution imaging probe for the formation and morphology of amyloid fibrils[J], 2020, 13(9)8.DOI10.1007s12274-020-2899-1.
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