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4×蛋白上样缓冲液（含巯基还原剂）
英文名称:SDS-PAGE loading buffer，4×(with β-Mercaptoethanol)
储存条件:-20℃，1 year
产品简介
| 英文名称 | SDS-PAGE loading buffer，4×(with β-Mercaptoethanol) |
| 中文名称 | 4×蛋白上样缓冲液（含巯基还原剂） |
| 外观(性状) | 蓝色粘稠液体 |
| 储存条件 | -20℃，1 year |
| 规格 | 10ml 100ml |
| 单位 | 瓶 |
保存： -20℃，有效期一年。
产品简介：
本产品适用于SDS-PAGE(SDS变性聚丙烯酰胺凝胶电泳)时作蛋白质上样用。其主要成份为SDS，β-巯基乙醇，溴酚蓝，缓冲盐溶液等。 SDS可与蛋白质结合使蛋白质－SDS复合物上带有大量的负电荷，这时蛋白质本身的电荷完全被SDS掩盖，消除了各种蛋白质本身电荷的差异，SDS还可以断开分子内和分子间的氢键，破坏蛋白质分子的二级和三级结构；β-巯基乙醇可以断开半胱氨酸残基之间的二硫键，破坏蛋白质的四级结构，消除了蛋白结构之间的差异。最终无电荷及结构上差异的蛋白（亚单位），电泳速度只是与其分子量大小有关；溴酚蓝用作电泳时的指示剂，可大概指示电泳结束的时间。
使用说明：
1. 请按每30μL蛋白样品加入10μL上样缓冲液的比例（4倍稀释）来使用。如果蛋白浓度过高，可用双蒸水稀释样品。
2. 混匀后，100℃水浴加热3-5分钟，使蛋白变性。
3. 冷却到室温后，10000-14000rpm离心2-5分钟，取上清直接上样电泳即可。
注意事项：
1. 聚丙烯酰胺凝胶浓度为8%时溴酚蓝指示条带的位置大概在30kd左右；胶浓度12%时，约在20kd左右；胶浓度15%时，大概在10kd。请根据自己目标条带来判断结果电泳时间。
2. 本试剂因含β-巯基乙醇，有一定的毒性，为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 蛋白上样缓冲液含有溴酚蓝指示剂，PH值受保存温度影响，在低温冻存状态下，溶液可能会呈现深棕色，不影响产品使用。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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5.0050
Publish_to：
Transboundary and Emerging Diseases
PMID：
34724348
Induction of Apoptosis via Inactivating PI3K/AKT Pathway in Colorectal Cancer Cells Using Aged Chinese Hakka Stir-Fried Green Tea Extract
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Author：
Xinyue Zhang, Haiying Huang, Shili Sun, Dongli Li, Lingli Sun, Qiuhua Li, Ruohong Chen, Xingfei Lai, Zhenbiao Zhang, Xi Zheng, Wing-Leung Wong, Shuai Wen
IF：
4.9270
Publish_to：
MOLECULES
PMID：
36500365
Anti-Melanogenic Effects of Takifugu flavidus Muscle Hydrolysate in B16F10 Melanoma Cells and Zebrafish
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Author：
Jinjin Hu, Bei Chen, Shuaijie Qu, Shuji Liu, Xiaoyu Yang, Kun Qiao, Yongchang Su, Zhihui Liu, Xiaoe Chen, Zhiyu Liu, Qin Wang
IF：
4.9000
Publish_to：
Marine Drugs
PMID：
38786597
Study on high moisture extruded pea protein isolate based on acid-induced process: Physicochemical properties, conformational changes and fibrous structure mechanism
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Author：
Xin Yang, Maodi Liang, Yihan Tang, Dingling Ma, Menghuan Li, Chenggang Yuan, Yanting Hou, Chaoyue Sun, Jie Liu, Qianqian Wei, Yongsheng Chang, Cuizhe Wang, Jun Zhang
IF：
4.8000
Publish_to：
FASEB JOURNAL
PMID：
37342904
VqBGH40a isolated from Chinese wild Vitis quinquangularis degrades trans-piceid and enhances trans-resveratrol
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Author：
Chaohui Yan, Na Yang, Xinqi Wang, Yuejin Wang
IF：
4.7290
Publish_to：
PLANT SCIENCE
PMID：
34315603
Interleukin-12 induces IFN-γ secretion and STAT signaling implying its potential regulation of Th1 cell response in Nile tilapia
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Author：
Haiyou Gao, Kang Li, Kete Ai, Ming Geng, Yi Cao, Ding Wang, Jialong Yang, Xiumei Wei
IF：
4.7000
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
37482205
Identification of a Yorkie homolog from Litopenaeus vannamei as a negative regulator in anti-WSSV immune response
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Author：
Jie Ren, Wenyang Lin, Hong Shi, Yiwen Jian, Lingwei Ruan
IF：
4.6220
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
36041626
Overexpressed miRNA-nov-1 promotes manganese-induced apoptosis in N27 cells by regulating Dhrs3 to activate mTOR signaling pathway
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Author：
Lina Wang, Liyu Su, Yuyan Cen, Jiao Li, Jianmin Yang, Yan Li
IF：
4.5000
Publish_to：
TOXICOLOGY
PMID：
36868551
Mining and characterization of oxidative stress-related binding proteins of parthenolide in Xanthomonas oryzae pv. oryzae
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Author：
Qian Zhou, Linwei Li, Fei Liu, Jun Hu, Yan Cao, Siwei Qiao, Yuxin Zhou, Bi Wang, Yihe Jia, Yu Chen, Shu Xu, Xu Feng
IF：
4.4620
Publish_to：
PEST MANAGEMENT SCIENCE
PMID：
35491536
miR-460b-5p Promotes Proliferation and Differentiation of Chicken Myoblasts and Targets RBM19 Gene
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Author：
Kai-Zhi Zhou, Peng-Fei Wu, Xuan-Ze Ling, Jin Zhang, Qi-Fan Wang, Xin-Chao Zhang, Qian Xue, Tao Zhang, Wei Han, Gen-Xi Zhang
IF：
4.4000
Publish_to：
POULTRY SCIENCE
PMID：
37980764
Mg(OH)2 nanoparticles enhance the antibacterial activities of macrophages by activating the reactive oxygen species
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Author：
Yong Zhu, Yifu Tang, Zhe Ruan, Yilong Dai, Zhaohui Li, Zhangyuan Lin, Shushan Zhao, Liang Cheng, Buhua Sun, Ming Zeng, Jianxi Zhu, Ruibo Zhao, Bangbao Lu, Haitao Long
IF：
4.3960
Publish_to：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
PMID：
34110087
circRNA3669 promotes goat endometrial epithelial cells proliferation via miR-26a/RCN2 to activate PI3K/AKT-mTOR and MAPK pathways1
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Author：
Xiao-rui LIU, Jiuzeng CUI, Meng-yao WEI, Xiao-fei WANG, Yue-xia LIU, Zhong-shi ZHU, Zhou Min, Gui BA, Lang-da SUO, Yu-xuan SONG, Lei ZhANG
IF：
4.3840
Publish_to：
Journal of Integrative Agriculture
PMID：
AT7867 Inhibits the Growth of Colorectal Cancer Stem-Like Cells and Stemness by Regulating the Stem Cell Maintenance Factor Ascl2 and Akt Signaling
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Author：
Yuchen Li, Yun Yuan, Luyao Yang, Hongqing Chen, Xufan Zhang, Tian Wen, Wenhao Liao, Maoyuan Zhao, Ziyi Zhao, Qiongying Hu
IF：
4.3000
Publish_to：
Stem Cells International
PMID：
36824410
In vivo antioxidant activity of Cinnamomum cassia leaf residues and their effect on gut microbiota of d-galactose-induced aging model mice
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Author：
Lingqi Chen, Tenggen Hu, Ruiqing Wu, Hong Wang, Hong Wu, Peng Wen
IF：
4.1250
Publish_to：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
36054514
Smart delivery of poly-peptide composite for effective cancer therapy
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Author：
Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu
IF：
4.1030
Publish_to：
Biomedical Materials
PMID：
34996052
Establishment and evaluation of targeted molecular screening model for the ryanodine receptor or sarco/endoplasmic reticulum calcium ATPase
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Author：
Xiaopeng Lu, Linlin Jiang, Li Chen, Wenwei Ding, Hua Wu, Zhiqing Ma
IF：
4.1000
Publish_to：
PEST MANAGEMENT SCIENCE
PMID：
38391097
Fusaricide is a Novel Iron Chelator that Induces Apoptosis through Activating Caspase-3
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Author：
Yaling Hui, Ting Tang, Jing Wang, Huanhuan Zhao, Hong-Ying Yang, Junmin Xi, Baoxin Zhang, Jianguo Fang, Kun Gao, Yueting Wu
IF：
4.0500
Publish_to：
JOURNAL OF NATURAL PRODUCTS
PMID：
34292737
Eimeria falciformis secretes extracellular vesicles to modulate proinflammatory response during interaction with mouse intestinal epithelial cells
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Author：
Olajide Joshua Seun, Xiong Ling, Yang Shunli, Qu Zigang, Xu Xiao, Yang Bin, Wang Jing, Liu Baohong, Ma Xueting, Cai Jianping
IF：
4.0470
Publish_to：
Parasites & Vectors
PMID：
35804396
Effects of NRF-1 and PGC-1α cooperation on HIF-1α and rat cardiomyocyte apoptosis under hypoxia
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Author：
Nan Niu, Hui Li, Xiancai Du, Chan Wang, Junliang Li, Jihui Yang, Cheng Liu, Songhao Yang, Yazhou Zhu, Wei Zhao
IF：
3.9130
Publish_to：
GENE
PMID：
35569770
Replication of standard bovine viral diarrhea strain OregonC24Va induces endoplasmic reticulum stress–mediated apoptosis of bovine trophoblast cells
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Author：
Yuan Naihan, Song Quanjiang, Jin Yan, Zhang Zhenhao, Wu Zheng, Sheng Xihui, Qi Xiaolong, Xing Kai, Xiao Longfei, Wang Xiangguo
IF：
3.8270
Publish_to：
CELL STRESS & CHAPERONES
PMID：
36441379
The mRNA Vaccine Expressing Single and Fused Structural Proteins of Porcine Reproductive and Respiratory Syndrome Induces Strong Cellular and Humoral Immune Responses in BalB/C Mice
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Author：
Luoyi Zhou, Ashenafi Kiros Wubshet, Jiangrong Zhang, Shitong Hou, Kaishen Yao, Qiuyi Zhao, Junfei Dai, Yongsheng Liu, Yaozhong Ding, Jie Zhang, Yuefeng Sun
IF：
3.8000
Publish_to：
Viruses-Basel
PMID：
38675887
miR-1285-3p targets TPI1 to regulate the glycolysis metabolism signaling pathway of Tibetan sheep Sertoli cells
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Author：
Xuejiao An, Taotao Li, Nana Chen, Huihui Wang, Manchun Su, Huibin Shi, Xinming Duan, Youji Ma
IF：
3.7520
Publish_to：
PLoS One
PMID：
36137140
Preparation and functional identification of various porcine cytokines
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Author：
Shanshan Yang, Qiuxia Cao, Kexin Yan, Chuanhong Wang, Xu Song, Xianyu Bian, Sufen Li, Zhenkong Cheng, Xuehan Zhang, Yi Wang, Rongli Guo, Xiaodu Wang, Houhui Song, Baochao Fan, Bin Li
IF：
3.7000
Publish_to：
CYTOKINE
PMID：
39922016
Regulatory role of dihydrotestosterone on BMP-6 receptors in granular cells of sheep antral follicles
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Author：
Jianshu Lv, Wenbo Ge, Ziqiang Ding, Jianlin Zeng, Wenjuan Wang, Hongwei Duan, Yong Zhang, Xingxu Zhao, Junjie Hu
IF：
3.6880
Publish_to：
GENE
PMID：
34838638
Overexpression of 15-Hydroxyprostaglandin Dehydrogenase Inhibits A549 Lung Adenocarcinoma Cell Growth via Inducing Cell Cycle Arrest and Inhibiting Epithelial-Mesenchymal Transition
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Author：
Weixuan Wang, Changmei Yang, Haiteng Deng
IF：
3.6020
Publish_to：
Cancer Management and Research
PMID：
34876851
A New lncRNA, lnc-LLMA, Regulates Lipid Metabolism in Pig Hepatocytes
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Author：
Lin Yu, Lina Tai, Jiayi Gao, Mingjie Sun, Siqi Liu, Tengda Huang, Jingsu Yu, Zhiwang Zhang, Weiwei Miao, Yixing Li, Ziyi Song, Haojie Zhang, Lei Zhou
IF：
3.5500
Publish_to：
DNA AND CELL BIOLOGY
PMID：
34981960
CircCDK1 blocking IGF2BP2每mediated m6A modification of CPPED1 promotes laryngeal squamous cell carcinoma metastasis via the PI3K/AKT signal pathway
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Author：
Jinling Li, Huan Cao, Jianwang Yang, Baoshan Wang
IF：
3.5000
Publish_to：
GENE
PMID：
37543219
Trimethylamine N-oxide promotes oxidative stress and lipid accumulation in macrophage foam cells via the Nrf2/ABCA1 pathway
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Author：
Luo ZhiSheng, Yu XiaoChen, Wang Chao, Zhao HaiYan, Wang Xinming, Guan XiuRu
IF：
3.4000
Publish_to：
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
PMID：
37932654
Inhibition of castration-resistant prostate cancer growth by genistein through suppression of AKR1C3
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Author：
Xiaoping Yu, Jiali Yan, Yulu Li, Jing Cheng, Lujie Zheng, Tianyu Fu, Yanfeng Zhu
IF：
3.3000
Publish_to：
Food & Nutrition Research
PMID：
36794010
SRSF3 facilitates replication of influenza A virus via binding and promoting the transport of viral mRNA
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Author：
Lingcai Zhao, Yingfei Li, Yongzhen Zhao, Qingzheng Liu, Yuanlu Lu, Jihui Ping
IF：
3.2460
Publish_to：
VETERINARY MICROBIOLOGY
PMID：
35063826
Quantitative proteomic analysis of local and systemic extracellular vesicles during Eimeria falciformis infectious cycle in the host
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Author：
Olajide Joshua Seun, Qu Zigang, Yang Shunli, Yang Bin, Xu Xiao, Wang Jing, Cai Jianping
IF：
3.2000
Publish_to：
Parasites & Vectors
PMID：
37759313
Dietary Restriction Attenuates Inflammation and Protects Mouse Skin from High-Dose Ultraviolet B Irradiation
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Author：
Duozhuang Tang, Jianying Wu, Yiting Wang, Hui Cui, Zhendong Tao, Lang Lei, Zhuangfa Zhou, Si Tao
IF：
3.1920
Publish_to：
REJUVENATION RESEARCH
PMID：
35152736
Effects of PHD and HSP90 on erythropoietin production in yak (Bos grunniens) renal interstitial fibroblast-like cells under hypoxia
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Author：
Cui Yan, Li Hui, Yu Si-Jiu, Afedo Seth Yaw, Bai Xue-Feng
IF：
3.1560
Publish_to：
JOURNAL OF MOLECULAR HISTOLOGY
PMID：
35084636
Unveiling the potent effect of vitamin D: harnessing Nrf2/HO-1 signaling pathways as molecular targets to alleviate urban particulate matter-induced asthma inflammation
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Author：
Ge Dandan, Chen Qihong, Xie Xiaohua, Li Qiyuan, Yang Yungang
IF：
3.1000
Publish_to：
BMC Pulmonary Medicine
PMID：
38273268
Anti‑malarial drug dihydroartemisinin downregulates the expression levels of CDK1 and CCNB1 in liver cancer
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Author：
Liyuan Hao, Shenghao Li, Qing Peng, Yinglin Guo, Jingmin Ji, Zhiqin Zhang, Yu Xue, Yiwei Liu, Xinli Shi
IF：
2.9670
Publish_to：
Oncology Letters
PMID：
34386075
Comparative analysis of translatomics and transcriptomics in the longissimus dorsi muscle of Luchuan and Duroc pigs
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Author：
Songtao Su, Hailong Hu, Kang Liu, Siqi Liu, Zupeng Luo, Jingsu Yu, Tianyu Jiang, Xiangling Li, Chang Sun, Lin Yu, Yuehui Liang, Lei Zhou
IF：
2.9000
Publish_to：
PLoS One
PMID：
40100799
Bioactive flavonoids and stilbenes from the leaf of Morus alba var. multicaulis
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Author：
Mimi Hao, Peijian Huang, Jingya Ruan, Fan Sun, Lifeng Han, Mengyang Liu, Yi Zhang, Tao Wang
IF：
2.8820
Publish_to：
FITOTERAPIA
PMID：
34450202
Profile of melatonin and its receptors and synthesizing enzymes in cumulus Coocyte complexes of the developing sheep antral follicle a potential estradiol-mediated mechanism
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Author：
Longfei Xiao, Junjie Hu, Liangli Song, Yong Zhang,
IF：
2.8520
Publish_to：
Reproductive Biology and Endocrinology
PMID：
30606208
ID3 regulates progesterone synthesis in bovine cumulus cells through modulation of mitochondrial function
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Author：
Zi-Bin Liu, Jia-Bao Zhang, Sheng-Peng Li, Wen-Jie Yu, Na Pei, Hai-Tao Jia, Ze Li, Wen-Fa Lv, Jun Wang, Nam-Hyung Kim, Bao Yuan, Hao Jiang
IF：
2.8000
Publish_to：
THERIOGENOLOGY
PMID：
37393744
Molecular characterization, expression patterns and cellular localization of BCAS2 gene in male Hezuo pig
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Author：
Yuran Tang, Bo Zhang, Haixia Shi, Zunqiang Yan, Pengfei Wang, Qiaoli Yang, Xiaoyu Huang, Shuangbao Gun
IF：
2.7000
Publish_to：
PeerJ
PMID：
37901468
Evodiamine Inhibits Gastric Cancer Cell Proliferation via PTEN-Mediated EGF/PI3K Signaling Pathway
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Author：
Ruichuang Yang, Jianxia Wen, Tao Yang, Chunmei Dai, Yanling Zhao
IF：
2.6300
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
34824590
Cloning, bioinformatics analysis and expression of the cysteine dioxygenase type 1 (CDO1) gene in domestic yak
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Author：
He Honghong, Xiong Xianrong, Fu Yuxin
IF：
2.6000
Publish_to：
Frontiers in Veterinary Science
PMID：
39493813
First Report of Blastocystis spp. Infection in Pet Birds in Henan Province, Central China
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Author：
Changwei Su, Xuefang Mei, Lai Wei, Fuqiang Zhang, Jiawen Wang, Yuan Chang, Mingyong Wang, Xiaowei Tian, Zhenchao Zhang, Xiangrui Li, Shuai Wang
IF：
2.5230
Publish_to：
VECTOR-BORNE AND ZOONOTIC DISEASES
PMID：
35834663
Wenyang-Tianjing-Jieyu Decoction Improves Depression Rats of Kidney Yang Deficiency Pattern by Regulating T Cell Homeostasis and Inflammation Level
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Author：
Tian Zhang, Jiexin Wang, Yi Wang, Linxi He, Shangbin Lv, Yiran Wang, Weihong Li
IF：
2.5000
Publish_to：
Neuropsychiatric Disease and Treatment
PMID：
38545129
Palmitic acid up regulates Gal‑3 and induces insulin resistance in macrophages by mediating the balance between KLF4 and NF‑κB
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Author：
Jing Li, Yu-Shan Mao, Fen Chen, Dong-Xia Xia, Tin-Qi Zhao
IF：
2.4470
Publish_to：
Experimental and Therapeutic Medicine
PMID：
34373714
FERMT1 knockdown attenuates inflammation induced by intracerebral hemorrhage in rats via NLRP3/NF-κB pathway
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Author：
Jianqiang WEI, Jing YIN, Ying CUI, Kaijie WANG, Mingyan HONG, Jianzhong CUI
IF：
2.4000
Publish_to：
EXPERIMENTAL ANIMALS
PMID：
36740252
Morphological identification and distribution comparison of telocytes in pituitary gland between normal and cryptorchid yaks
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Author：
Qi Yumei, Yuan Ligang, Zeng Jianlin, Wang Xiaofen, Ma Long, Lv Jinghan
IF：
2.3000
Publish_to：
BMC Veterinary Research
PMID：
39394144
Identification and expression analysis of a new small ubiquitin-like modifier from Taenia pisiformis
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Author：
Shaohua Zhang, Bingtian Jin, Weijia Liang, Aijiang Guo, Xuenong Luo, Lixia Pu, Xiaoqing Chen, Xuepeng Cai, Shuai Wang
IF：
2.1320
Publish_to：
EXPERIMENTAL PARASITOLOGY
PMID：
36209934
Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice
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Author：
Lu Juan, Yu Yue, Wang Xiao-jing, Chai Rui-ping, Lyu Xin-kai, Deng Ming-hui, Hu Mei-geng, Qi Yun, Chen Xi
IF：
1.9780
Publish_to：
Chinese Journal of Integrative Medicine
PMID：
34241804
Effect of dihydrotestosterone on melatonin secretion and the expression of melatonin receptors and apoptosis-related factors in sheep epididymides
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Author：
Jianlin Zeng, Wenbo Ge, Hongwei Duan, Jianshu Lv, Ziqiang Ding, Wenjuan Wang, Yong Zhang, Xingxu Zhao, Junjie Hu
IF：
1.8580
Publish_to：
REPRODUCTION IN DOMESTIC ANIMALS
PMID：
35775862
The Impact of miR-141 overexpression on apoptosis and proliferation in Tscca and Tca8113 Cell Lines
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Author：
Tao Huang, Bo Shao, Zhong-Cheng Gong
IF：
1.8000
Publish_to：
Journal of Stomatology Oral and Maxillofacial Surgery
PMID：
39909369
MiR‐766‐3p Inhibit the Proliferation, Stemness, and Cell Cycle of Pancreatic Cancer Cells Through the MAPK/ERK Signaling Pathway
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Author：
Zhipeng Quan, Ziwei Yin, Yuelin Huang, Xuemei Huang, Hao Huang, Qingrong Mo, Jianhua Gong, Lingyun Liu, Yi Zhou, Yaqun Yu
IF：
1.5000
Publish_to：
Molecular Genetics & Genomic Medicine
PMID：
39692209
Cardiac-Specific Gene Editing via an AAV9-Tnnt2-SaCas9-miR122TS Vector
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Author：
Luzi Yang, Congting Guo, Yueshen Sun, Yuxuan Guo
IF：
1.0000
Publish_to：
Bio-protocol
PMID：
40084085
Generation of Stable Cell Lines of APP695 (K595N, M596L) Gene Over-Expression via Lentiviral Over-Expression System
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Author：
Huitao Zhang, Hao Rui, Yu Zitong, Hu Shi, Ren Jingyi, Hong Yanhang, Wang Shifan, Sun Huihui, Zhang Pengcheng, Zhang Yi, Yang Hui, Ding Juan
IF：
0.5000
Publish_to：
BIOLOGY BULLETIN
PMID：
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