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产品简介
| 英文名称 | TE Buffer (pH 8.0) |
| 储存条件 | Store at RT，1 year. |
| 规格 | 100ml 500ml |
| 单位 | 瓶 |
TE缓冲液是Tris +EDTA缓冲液，这种缓冲液我们一般用作溶解剂或保持剂，主要是调控PH。
TAE是一种电泳缓冲液，主要用于DNA分子的电泳。
TE组成浓度：
10mM Tris-HCl
1mM EDTA PH=8.0
配制方法：
量取下列溶液于500ml烧杯中 1M Tris-HCl Buffer PH=8.0 5ml 0.5M EDTA PH=8.0 1ml 向烧杯中加入约400mldd H2O均匀混合；将溶液定容到500ml后；室温保存。
TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量（TBE中电泳时测出的相对分子质量会大于实际分子质量），且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%，电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果，此外，回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小，长时间电泳（如过夜）不可选用，除非有循环装置使两极的缓冲液得到交换。
50×TAE Buffer 配制方法：
称量Tris 242g，Na2EDTA.2H2O 37.2g 于1L烧杯中；
向烧杯中加入约800ml去离子水，充分搅拌均匀；
加入57.1ml的冰乙酸，充分溶解；
加去离子水定容至1L后，室温保存。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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技术问答
（5）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
京公网安备 11011202000391号
这个不是无酶的，可以用来溶解sgRNA吗?2023-12-28 20:25:28
您好，是不适用的。
是10×的嘛?2023-10-11 16:55:19
您好，TE缓冲液是Tris+EDTA缓冲液，这种缓冲液我们一般用作溶解剂或保持剂，和电泳用的TAE电泳液是不同的。
TE buffer是无核酶的吗，可以用来稀释引物吗?2023-06-27 16:47:36
您好，该产品没有做无酶处理，可以用来稀释引物。理论上，应该使用无核酸酶的水溶解引物，但因其 pH 很难保持稳定在 PH≥7.0，故非常不推荐，说明如下：DEPC（焦碳酸二乙酯）能够与 RNase 的活性基团进行反应从而抑制其活性，用来防止 RNA 降解。而常规的引物属于单链 DNA，因此是没有必要用到 DEPC 的，并且，DEPC 在高温高压处理时能够降解产生 CO2，降低水的 pH 值，对 DNA的保存是不利的。所以不推荐使用 DEPC 水溶解引物，除非您的后续反应体系中有 RNA 参与（如配制逆转录引物）。
TE buffer是无菌的吗?2023-05-29 13:35:30
您好，该产品经过无菌处理，是无菌的。
使用这个缓冲液如何配制0.1×TE buffer?2023-04-18 17:26:04
您好，是即用型的，不需要稀释使用。