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青霉素-链霉素-两性霉素B混合溶液（100×）
英文名称:Penicillin / Streptomycin / Amphotericin B，sterile solution
储存条件:-20℃，避光保存，有效期1年。
产品简介
| 别名 | 三抗 真菌抗生素 青霉素-链霉素-两性霉素B混合溶液 Antibiotic-Antimycotic， 100X |
| 英文名称 | Penicillin / Streptomycin / Amphotericin B，sterile solution |
| 中文名称 | 青霉素-链霉素-两性霉素B混合溶液（100×） |
| 储存条件 | -20℃，避光保存，有效期1年。 |
| 规格 | 100ml 10*100ml |
| 单位 | 瓶 |
青霉素-链霉素-两性霉素B混合溶液(100×三抗)抗菌谱： 细菌、真菌和酵母。
细胞培养基中有时加入适量浓度的抗生素，可以有效防止微生物的污染。目前，最常采用的抗生素为青霉素-链霉素混合溶液(100×双抗)。青霉素-链霉素-两性霉素B(100×三抗) (Penicillin- Streptomycin-Amphotericin B Solution, 100×)是专门用于细胞培养的三抗，经过滤除菌，可以直接添加到 细胞培养液内。 青霉素-链霉素-两性霉素B混合溶液(100×三抗)中青霉素含量10kU/ml，链霉素含量10mg/ml，两性霉素B含量25µg/ml。该溶液用0.9%NaCl或PBS配制， 在细胞培养液中推荐的青霉素工作浓度为100U/ml，链霉素工作浓度为0.1mg/ml，两性霉素B工作浓度为0.25µg/ml即按照100倍稀释使用即可。 一个包装即100ml青霉素-链霉素-两性霉素B混合溶液(100×三抗)可以配制10L细胞培养液。
青霉素-链霉素-两性霉素B混合溶液(100×三抗)可以参考如下两种方法之一使用：
1. 在无菌的细胞培养液中直接添加：按照每500ml细胞培养液添加5ml的比例加入青霉素-链霉素两性霉素B混合溶液(100×三抗)，混匀即可使用。
2. 配制细胞培养液时加入，然后再过滤除菌：配制细胞培养液时按照每配制1L细胞培养液加入10ml 的比例加入青霉素-链霉素-两性霉素B混合溶液(100×三抗)，配制完成后过滤除菌即可使用。
注意事项：
1. 尽量减少反复冻融的次数，以免效率下降。
2. 注意无菌操作，尽量避免污染。
3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Publish_to：
JOURNAL OF BIOLOGICAL CHEMISTRY
PMID：
34534547
Exosomal circZNF800 Derived from Glioma Stem-like Cells Regulates Glioblastoma Tumorigenicity via the PIEZO1/Akt Axis
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Author：
Zhang Ning, Wu Pengfei, Mu Maolin, Niu Chaoshi, Hu Shanshan
IF：
5.1000
Publish_to：
MOLECULAR NEUROBIOLOGY
PMID：
38324181
Mycobacterium tuberculosis/Mycobacterium bovis triggered different variations in lipid composition of Bovine Alveolar Macrophages
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Author：
Chen Yuqi, Ma Huiya, Duan Yangbo, Ma Xueyan, Tan Lihui, Dong Jianjian, Jin Chenkai, Wei Rong
IF：
4.9960
Publish_to：
Scientific Reports
PMID：
35908111
Electroactive Oxidized Alginate/Gelatin/MXene (Ti3C2Tx) Composite Hydrogel with Improved Biocompatibility and Self-Healing Property
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Author：
Hui Zhu, Weitao Dai, Liming Wang, Cong Yao, Chenxi Wang, Bingsong Gu, Dichen Li, Jiankang He
IF：
4.9670
Publish_to：
Polymers
PMID：
36146053
Cath-KP, a novel peptide derived from frog skin, prevents oxidative stress damage in a Parkinson’s disease model
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Author：
Huanpeng Lu, Jinwei Chai, Zijian Xu, Jiena Wu, Songzhe He, Hang Liao, Peng Huang, Xiaowen Huang, Xi Chen, Haishan Jiang, Shaogang Qu, Xueqing Xu
IF：
4.9000
Publish_to：
ZOOLOGICAL RESEARCH
PMID：
38114437
HucMSC-EVs Facilitate In Vitro Development of Maternally Aged Preantral Follicles and Oocytes
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Author：
Zhang Ying-Yi, Yang Weijie, Zhang Yi, Hu Zhanhong, Chen Yingyan, Ma Yerong, Yang Anran, Shi Zhan, Zhou Hanjing, Ren Peipei, Shi Libing, Jin Jiamin, Rong Yan, Tong Xiaomei, Zhang Yin-Li, Zhang Songying
IF：
4.8000
Publish_to：
Stem Cell Reviews and Reports
PMID：
36862330
In vitro acne disease model from inertial focusing effect for studying the interactions between sebocyte glands and macrophages
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Author：
Tan Tang, Ye Xu, Lelin Wang, Peipei Zhang
IF：
4.7000
Publish_to：
Biotechnology Journal
PMID：
37477791
Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors
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Author：
Yuxiang Hu, Ziqi He, Shuangai Liu, Wenwen Ying,Yifan Chen, Manli Zhao, Min He, Xuan Wu,Yinbing Tang, Weizhong Gu, Meidan Ying, Jinhu Wang,Ting Tao
IF：
4.6000
Publish_to：
iScience
PMID：
39319271
Toxicities of polystyrene microplastics (MPs) and hexabromocyclododecane (HBCD), alone or in combination, to the hepatopancreas of the whiteleg shrimp, Litopenaeus vannamei
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Author：
Zhihong Gong, Qi Zhang, Juanjuan Liu, Guobin Hu, Songlin Chen, Na Wang
IF：
4.5000
Publish_to：
AQUACULTURE
PMID：
The lncRNA NEAT1 Mediates Neuronal Cell Autophagy and Related Protein Expression After Cerebral Ischemia‒Reperfusion Injury
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Author：
Xia Wenbo, Ni Xunran, Su Qian, Jia Kejuan, Zhang Yanli, Meng Delong, Wang Xin, Su Zhiqiang
IF：
4.4140
Publish_to：
NEUROCHEMICAL RESEARCH
PMID：
36512294
miR-460b-5p Promotes Proliferation and Differentiation of Chicken Myoblasts and Targets RBM19 Gene
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Author：
Kai-Zhi Zhou, Peng-Fei Wu, Xuan-Ze Ling, Jin Zhang, Qi-Fan Wang, Xin-Chao Zhang, Qian Xue, Tao Zhang, Wei Han, Gen-Xi Zhang
IF：
4.4000
Publish_to：
POULTRY SCIENCE
PMID：
37980764
Gradient coating of extracellular matrix derived from endothelial cells on aligned PCL nanofibers for rapid endothelialization
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Author：
Ziyi Zhou,Yijing Lin,Na Liu,Yiming Zhang,Bing Li,Yuanfei Wang
IF：
4.3000
Publish_to：
Frontiers in Bioengineering and Biotechnology
PMID：
39845372
Effects of Baicalin on Gout Based on Network Pharmacology, Molecular Docking, and in vitro Experiments
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Author：
Chunliu Liu, Wei Liu, Hang Lu, Yihua Fan, Aihua Wang
IF：
4.2000
Publish_to：
Journal of Inflammation Research
PMID：
39925939
MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene
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Author：
Mingliang He, Weibo Zhang, Shan Wang, Ling Ge, Xiukai Cao, Shanhe Wang, Zehu Yuan, Xiaoyang Lv, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Wei Sun
IF：
4.1410
Publish_to：
Genes
PMID：
35328074
A Novel Magnetic Manipulation Promotes Directional Growth of Periodontal Ligament Stem Cells
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Author：
Weiguang Li, Weidong Tian, Yafei Wu, Shujuan Guo
IF：
4.1000
Publish_to：
Tissue Engineering Part A
PMID：
37603495
Eimeria falciformis secretes extracellular vesicles to modulate proinflammatory response during interaction with mouse intestinal epithelial cells
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Author：
Olajide Joshua Seun, Xiong Ling, Yang Shunli, Qu Zigang, Xu Xiao, Yang Bin, Wang Jing, Liu Baohong, Ma Xueting, Cai Jianping
IF：
4.0470
Publish_to：
Parasites & Vectors
PMID：
35804396
RCC1 functions as a tumor facilitator in clear cell renal cell carcinoma by dysregulating cell cycle, apoptosis, and EZH2 stability
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Author：
Yunfei Wu, Zhijie Xu, Xiaoyi Chen, Guanghou Fu, Junjie Tian, Baiye Jin
IF：
4.0000
Publish_to：
Cancer Medicine
PMID：
37747077
Therapeutic targets of formononetin for treating prostate cancer at the single-cell level
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Author：
Jiawei Li, Zhaoquan Huang, Ping Wang, Rong Li, Li Gao, Keng Po Lai
IF：
3.9000
Publish_to：
Aging-US
PMID：
38874510
Morroniside ameliorates glucocorticoid-induced osteoporosis and promotes osteoblastogenesis by interacting with sodium-glucose cotransporter 2
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Author：
Hou-Zhi Yang, Runbei Dong, Yutao Jia, Yuqiao Li, Gan Luo, Tianhao Li, Yao Long, Shuang Liang, Shanshan Li, Xin Jin, Tianwei Sun
IF：
3.8000
Publish_to：
PHARMACEUTICAL BIOLOGY
PMID：
36786302
PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomes
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Author：
Zhixin Li, Tongzhu Jin, Ruoxuan Yang, Jiayu Guo, Zhihui Niu, Huiying Gao, Xiaoying Song, Qing Zhang, Zhiwei Ning, Lingxue Ren, Yan Wang, Xingxing Fan, Haihai Liang, Tianyu Li, Wenxin He
IF：
3.7000
Publish_to：
EXPERIMENTAL CELL RESEARCH
PMID：
37330182
Plectin regulates Wnt signaling mediated-skeletal muscle development by interacting with Dishevelled-2 and antagonizing autophagy
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Author：
Huadong Yin, Shunshun Han, Can Cui, Yan Wang, Diyan Li, Qing Zhu
IF：
3.6880
Publish_to：
GENE
PMID：
33705811
Jade powder/PLGA composite microspheres for improved performance as potential bone repair drug carrier
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Author：
Xinlu Zhang, Zelin Liao, Chong Han, Junliang Wu, Yifei Yu, Xingyu Chen, Hao Gong, Gaohong He, Xiujuan Zhang
IF：
3.6000
Publish_to：
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
PMID：
39535251
Anti-inflammatory actions of acetate, propionate, and butyrate in fetal mouse jejunum cultures ex vivo and immature small intestinal cells in vitro
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Author：
Shengnan Huang, Yanan Gao, Ziwei Wang, Xue Yang, Jiaqi Wang, Nan Zheng
IF：
3.5530
Publish_to：
Food Science & Nutrition
PMID：
35154692
The Modulation of Blue-Light-Induced Inflammation, Intracellular Lipid Secretion, and Oxidative Stress in Sebocytes with Cannabidiol
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Author：
Yanran Wei, Tan Tang, Jitao Liu, Siyi Yu, Huo Tong, Peipei Zhang
IF：
3.5210
Publish_to：
PHOTOCHEMISTRY AND PHOTOBIOLOGY
PMID：
36524438
Ginsenoside Rg1 inhibits multiple myeloma and overcomes bortezomib resistance through AMPK-mTOR pathway
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Author：
Li Lin, Dong Chen, Shuangyue Li, Tiantian Wang
IF：
3.4000
Publish_to：
Heliyon
PMID：
39071579
Pseudocochlodinium profundisulcus Resting Cysts Detected in the Ballast Tank Sediment of Ships Arriving in the Ports of China and North America and the Implications in the Species’ Geographic Distribution and Possible Invasion
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Author：
Lixia Shang, Xinyu Zhai, Wen Tian, Yuyang Liu, Yangchun Han, Yunyan Deng, Zhangxi Hu, Ying Zhong Tang
IF：
3.3900
Publish_to：
International Journal of Environmental Research and Public Health
PMID：
35010560
Andrographolide attenuates sepsis-induced acute kidney injury by inhibiting ferroptosis through the Nrf2/FSP1 pathway.
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Author：
Yixin Zhang, Youcheng Zeng, Ming Huang, Guodong Cao, Liang Lin, Xiaoyue Wang, Qinghong Cheng
IF：
3.3000
Publish_to：
FREE RADICAL RESEARCH
PMID：
38478853
HSP90 inhibitors 17-AAG and VER-82576 inhibit porcine deltacoronavirus replication in vitro
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Author：
Yujia Zhao, Dai Xiao, Luwen Zhang, Daili Song, Rui Chen, Shiqian Li, Yijie Liao, Yimin Wen, Weizhe Liu, Enbo Yu, Yiping Wen, Rui Wu, Qin Zhao, Senyan Du, Xintian Wen, Sanjie Cao, Xiaobo Huang
IF：
3.2930
Publish_to：
VETERINARY MICROBIOLOGY
PMID：
34954542
Structural and functional characterization of turbot pparγ: Activation during high temperature and regulation of lipid metabolism
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Author：
Tingting Zhao, Aijun Ma, Zhihui Huang, Zhifeng Liu, Zhibin Sun, Xinan Wang, Rongjing Xu
IF：
3.1890
Publish_to：
JOURNAL OF THERMAL BIOLOGY
PMID：
36031207
Molecular characteristics of extended-spectrum -lactamase-producing Escherichia coli isolated from the rivers and lakes in Northwest China
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Author：
Haixia Liu, Hongchao Zhou, Qinfan Li, Qian Peng, Q
IF：
3.1040
Publish_to：
BMC Microbiology
PMID：
30286725
IL-13/IL-13RA2 signaling promotes colorectal cancer stem cell tumorigenesis by inducing ubiquitinated degradation of p53
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Author：
Mingliang He, Xiaoyang Lv, Xiukai Cao, Zehu Yuan, Kai Quan, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Yutao Li, Shanhe Wang, Wei Sun
IF：
3.0000
Publish_to：
Animals
PMID：
37370543
pparβ regulates lipid catabolism by mediating acox and cpt-1 genes in Scophthalmus maximus under heat stress
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Author：
Zhao Tingting, Ma Aijun, Huang Zhihui, Liu Zhifeng, Sun Zhibin, Zhu Liguang, Chang Haowen
IF：
2.9000
Publish_to：
FISH PHYSIOLOGY AND BIOCHEMISTRY
PMID：
38386263
Maraviroc enhances Bortezomib sensitivity in multiple myeloma by inhibiting M2 macrophage polarization via PI3K/AKT/RhoA signaling pathway in macrophages
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Author：
Yang Huiye, He Yuchan, Qu Fujun, Zhu Jie, Deng Liyuan, Jiang Fang, Wu Xianyi, Chen Yixuan, Kashif Ali, Wang Xiaotao
IF：
2.8000
Publish_to：
Cell Division
PMID：
39953613
LncRNA BACE1-AS promotes the progression of osteosarcoma through miR-762/SOX7 axis
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Author：
Wang Chunlei, Zhang Tao, Yang Lin, Na Xinyu, Qu Yanlong
IF：
2.7420
Publish_to：
MOLECULAR BIOLOGY REPORTS
PMID：
35332412
Mechanisms of Mitochondria-Mediated Apoptosis During Eimeria tenella Infection
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Author：
Rui Bai, Hui Wang, Tiantian Yang, Yuqi Yan, Shuying Zhu, Chenyang Lv, Yang Pei, Jiale Guo, Jianhui Li, Xiaozhen Cui, Xiaoling Lv, Mingxue Zheng
IF：
2.7000
Publish_to：
Animals
PMID：
40003058
Evaluation the protective role of baicalin against H2O2-driven oxidation, inflammation and apoptosis in bovine mammary epithelial cells
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Author：
Xiaohui Kong, Mingyan Wang, Zhiheng Guo, Xingda Yang, Hongxia Lian, Tengyun Gao, Liyang Zhang, Tong Fu
IF：
2.6000
Publish_to：
Frontiers in Veterinary Science
PMID：
39726580
Dihydroquercetin improves the proliferation of porcine intestinal epithelial cells via the Wnt/β-catenin pathway
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Author：
Guowei Liu, Yongxia Fang, Yiyu Zhang, Min Zhu
IF：
2.5000
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
39083968
Non-targeted Metabolomics Reveals the Potential Role of MFSD8 in Metabolism in Human Endothelial Cells
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Author：
Xiang Qin, Chen Yongjun, Cheng Xu, Fang Xinxiang, Liu Yuxiang, Huang Yujie, He Binsheng, Tang Liang, Li Jianming
IF：
2.4000
Publish_to：
MOLECULAR BIOTECHNOLOGY
PMID：
39992484
Eimeria falciformis extracellular vesicles differentially express host cell lncRNAs
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Author：
Joshua S. Olajide, Zigang Qu, Shunli Yang, Bin Yang, Xiao Xu, Jing Wang, Jianping Cai
IF：
2.2000
Publish_to：
JOURNAL OF EUKARYOTIC MICROBIOLOGY
PMID：
38073253
MiR-206 regulates the progression of osteoporosis via targeting HDAC4
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Author：
Lu Zhiyuan, Wang Dawei, Wang Xuming, Zou Jilong, Sun Jiabing, Bi Zhenggang
IF：
2.1750
Publish_to：
EUROPEAN JOURNAL OF MEDICAL RESEARCH
PMID：
33461610
Preliminary study of melatonin in the proliferation and apoptosis of Hu sheep dermal papilla cells in vitro
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Author：
Mingliang He, Hui Zhou, Tingyan Hu, Xiaoyang Lv, Shanhe Wang, Xiukai Cao, Zehu Yuan, Kai Quan, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Wei Sun
IF：
2.1410
Publish_to：
ANIMAL BIOTECHNOLOGY
PMID：
36384387
Targeting NEDD8 in pediatric acute myeloid leukemia: an integrated bioinformatics and experimental approach
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Author：
Jian Sun, Cui Liu, Guangli Yang, Qian Li, Yang An, Yin Zhu, Pingping Zhang, Yaning Guan, Chang Peng, Zuochen Du, Pei Huang, Yan Chen
IF：
2.0000
Publish_to：
Hematology
PMID：
40103351
PHGDH promotes the proliferation and differentiation of primary chicken myoblasts
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Author：
L. Chen, Y. L. Wu, H. Ding, K. Z. Xie, T. Zhang, G. X. Zhang, J. Y. Wang
IF：
1.8920
Publish_to：
BRITISH POULTRY SCIENCE
PMID：
35383521
Recombinant α-Toxin BmK-M9 Inhibits Breast Cancer Progression by Regulating β-Catenin In Vivo
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Author：
Chen Wenlin, Cha Zhuocen, Huang Saijun, Liu Ruimin, Chen Jiayi, Kamau Peter Muiruri, Lu Xingjia, Li Bowen, Liu Dequan
IF：
1.8000
Publish_to：
CELL BIOCHEMISTRY AND BIOPHYSICS
PMID：
40080350
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