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聚乙二醇6000

CAS:No.25322-68-3

英文名称:PEG6000

分子式:HO(CH2CH2O)nH

分子量:≈6000

储存条件:Store at RT,2 years

聚乙二醇6000
满3999赠露营车
货号:
IP9110
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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使用本产品的案例(仅供参考

In Vitro

Lentivirus production and generation of clonal cell lines(用PEG6000沉淀上清中的shRNA病毒

Concentrated shRNA lentiviruses were obtained by precipitating viral containing supernatants with PEG6000 (Solarbio).  

来源文献:Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z, Zou W, Chen B. Real-time imaging of RNA polymerase I activity in living human cells. J Cell Biol. 2023 Jan 2;222(1):e202202110. doi: 10.1083/jcb.202202110. Epub 2022 Oct 25. PMID: 36282216; PMCID: PMC9606689.


Lentivirus production and generation of clonal cell lines.(病毒上清液与PEG 6000按2:1的比例混合,病毒在4°C储存12 h后浓缩;PBS重悬病毒

After 60 h of transfection, virus supernatant was harvested and mixed with PEG 6000 (Solarbio) in a ratio of 2:1.    The virus was then concentrated after 12 h storage at 4°C. Finally, the virus precipitation was resuspended in PBS and stored at -80°C.

来源文献:Xu H, Wang J, Deng Y, Hou F, Fu Y, Chen S, Zou W, Pan D, Chen B. Two-Color CRISPR Imaging Reveals Dynamics of Herpes Simplex Virus 1 Replication Compartments and Virus-Host Interactions. J Virol. 2022 Dec 21;96(24):e0092022. doi: 10.1128/jvi.00920-22. Epub 2022 Dec 1. PMID: 36453882; PMCID: PMC9769385.


Plant( YM21棉花幼苗,10% PEG6000 ,干旱胁迫

The 300 mM NaCl (Solarbio, Beijing, China) solution and 10% PEG6000 (Solarbio, Beijing, China) solution were used to simulate salt stress and drought stress, respectively.  The leaves and roots were collected at 0, 3, 6, 12 and 24 h under different abiotic stresses, and the samples were quick-frozen in liquid nitrogen.  To analyze the tissue-specific expression level of GhTIP1;1-like in TM-1, roots, stems and leaves were collected from seedlings, and buds, sepals, petals, stamens and fiber samples.

来源文献:Cheng G, Wang M, Zhang L, Wei H, Wang H, Lu J, Yu S. Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis. Int J Mol Sci. 2022 Jan 25;23(3):1361. doi: 10.3390/ijms23031361. PMID: 35163287; PMCID: PMC8836057.


备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
Cheng G, et al. Int J Mol Sci. 2022 Jan 25;23(3)1361.
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