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重组人胰岛素

CAS:No.11061-68-0

英文名称:Recombinant Human Insulin

分子式:C257H383N65O77S6

分子量:5807.57

储存条件:Powder:-20℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

重组人胰岛素
满3999赠露营车
货号:
IR1490
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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本产品为制备的全长序列的人胰岛素,是一种重组技术生产的由51个氨基酸残基组成的多肽,调节血液中的葡萄糖水平。可用于细胞培养和动物实验。

不溶于水,可溶于稀醋酸或者稀盐酸,建议按照单次使用剂量分装冻存于-20℃,避免反复冻融。

本产品为非无菌包装,溶解后建议过滤除菌。



使用本产品的应用案例(仅供参考

In Vitro

Cell(3T3-L1 preadipocytes cells,10  μg/ml insulin)

During differentiation, 3T3-L1 cells were cultured to confluency for 48 h and then incubated in differentiation medium consisting of insulin (10 μg/ml) (Solarbio), dexamethasone (0.25 μM), 3-isobutyl-1-methylxanthine (0.25 mM) (IBMX). Two days later, the medium was switched to DMEM consisting of 10 μg/ml insulin and changed every other day till full differentiation. 

数据来源文献:Li Z, Xu K, Zhao S, Guo Y, Chen H, Ni J, Liu Q, Wang Z. SPATA4 improves aging-induced metabolic dysfunction through promotion of preadipocyte differentiation and adipose tissue expansion. Aging Cell. 2021 Jan;20(1):e13282. doi: 10.1111/acel.13282. Epub 2020 Dec 13. PMID: 33314576; PMCID: PMC7811838.


Cell(0.01 g/L insulin)

The adipogenic medium was the complete culture medium supplemented with 1 μM dexamethasone (Solarbio), 0.2 mM indomethacin (Solarbio), 0.01 g/L insulin (Solarbio), and 0.5 mM isobutyl-methylxanthine (Solarbio).

数据来源文献:Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821.


In Vivo

Mice(0.75 U/kg  insulin )

For insulin tolerance test (ITT), mice were fasted for 4 h and then injected intraperitoneally with insulin (0.75 U/kg body weight) (Solarbio) and blood glucose was measured at 0, 15, 30, 60 min using a glucometer.

数据来源文献:Li Z, Xu K, Zhao S, Guo Y, Chen H, Ni J, Liu Q, Wang Z. SPATA4 improves aging-induced metabolic dysfunction through promotion of preadipocyte differentiation and adipose tissue expansion. Aging Cell. 2021 Jan;20(1):e13282. doi: 10.1111/acel.13282. Epub 2020 Dec 13. PMID: 33314576; PMCID: PMC7811838.


备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
暂无详情
Li Z, et al. Med Oncol. 2015 Jun;32(6):577.
Li Z, et al. Aging Cell. 2021 Jan;20(1)e13282.
注意:
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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