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曲拉通x-100

CAS:No.9002-93-1

英文名称:Triton x-100

分子式:No data available

储存条件:Store at RT,2 years

曲拉通x-100
货号:
IT9100
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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Triton X-100 是一种非离子表面活性剂。据报道,Triton X-100 可用于某些疫苗的赋形剂。


In Vitro
NRF2 positive cells in the knee joint ,Paraffin sections were permeated with 0.5% TritonX-100

Paraffin sections were permeated with 0.5% TritonX-100, and incubated with the NRF2 primary antibody at 4 °C overnight. Incubated the secondary antibody for 1 h and DAPI for 5 min at room temperature. The images were captured by fluorescence microscope (Leica, Wetzlar, Germany).

来源文献:Yang J, Chen D, He Q, Chen B, Pan Z, Zhang G, Li M, Li S, Xiao J, Wang H, Chen P, An Z. Arctiin alleviates knee osteoarthritis by suppressing chondrocyte oxidative stress induced by accumulated iron via AKT/NRF2/HO-1 signaling pathway. Sci Rep. 2024 Dec 30;14(1):31935. doi: 10.1038/s41598-024-83383-7. PMID: 39738432; PMCID: PMC11685860.


CellMC3T3-E1 cells0.5% Triton X-100 (Solarbio) for 5 min

To mimic the bone matrix, MC3T3-E1 cells were induced for osteogenic differentiation in MEM-α media containing 10% of FBS, 10 mM β-glycerophosphate (Solarbio), and 50 μg/ml of ascorbic acid (Solarbio) for 9 days. Subsequently, the cultured MC3T3-E1 cells were treated with 20 mM NH4OH and 0.5% Triton X-100 (Solarbio) for 5 min to form the bone matrix layer. GFP expressing breast cancer cells (2 × 105 cells/well) were added into each bone matrix layer and incubated for 15  min. After aspirating floating cells, the adhering cells were washed with PBS and counted under a fluorescent microscope.

来源文献:Sun L, Zhang Y, Chen G, Ji Y, Ma Q, Qiao X, Wu S, Zhou L, Bu J, Zhu X, Zhang X, Jiang X, Liu C, Li X, Liu Y, Yang Y, Liu C. Targeting SOST using a small-molecule compound retards breast cancer bone metastasis. Mol Cancer. 2022 Dec 29;21(1):228. doi: 10.1186/s12943-022-01697-4. Erratum in: Mol Cancer. 2025 Apr 16;24(1):117. doi: 10.1186/s12943-025-02322-w. PMID: 36581888; PMCID: PMC9798707.


CellBMSCs0.1% of Triton-X 100

To induce osteoblast differentiation, the isolated mouse BMSCs (2 × 105 cells/well) were treated with DMSO or S6 (8 μM) in a modified α-MEM containing 10% of FBS,

50 μg/ml L-ascorbic acid, 1 mM dexamethasone and 1 M β-glycerophosphate in 12-well plates. The media were changed every other day for 14 days. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% of Triton-X 100, denatured again with 0.1% PBST, and stained with alkaline phosphatase (ALP) using a specific kit .

来源文献:Sun L, Zhang Y, Chen G, Ji Y, Ma Q, Qiao X, Wu S, Zhou L, Bu J, Zhu X, Zhang X, Jiang X, Liu C, Li X, Liu Y, Yang Y, Liu C. Targeting SOST using a small-molecule compound retards breast cancer bone metastasis. Mol Cancer. 2022 Dec 29;21(1):228. doi: 10.1186/s12943-022-01697-4. Erratum in: Mol Cancer. 2025 Apr 16;24(1):117. doi: 10.1186/s12943-025-02322-w. PMID: 36581888; PMCID: PMC9798707.






备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
Sun L,.et al. Mol Cancer 2022 Dec 29;21(1):228. doi: 10.1186/s12943-022-01697-4. Erratum in: Mol Cancer. 2025 Apr 16;24(1):117. doi: 10.1186/s12943-025-02322-w.
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