游离胆固醇(FC)含量检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Free Cholestenone(FC) Content Assay Kit
储存条件:Store at 2-8℃,6 months
产品简介
自备试剂 | 该试剂盒实验过程中需自备试剂,详情见网站说明书 |
中文名称 | 游离胆固醇(FC)含量检测试剂盒 |
英文名称 | Free Cholestenone(FC) Content Assay Kit |
别名 | 游离胆固醇含量测定试剂盒 FC Kit 游离胆固醇(FC)含量测定试剂盒 游离胆固醇(FC)含量测试盒 |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at 2-8℃,6 months |
规格 | 100T/96S |
单位 | 盒 |
测定意义:FC是构成细胞膜的主要成分,也是合成肾上腺皮质激素、性激素、胆汁酸及维生素D等生理活性物质的重要原料。FC浓度可作为脂代谢的指标。
测定原理: FC氧化酶催化FC生成△4-胆甾烯酮和H2O2,过氧化物酶催化H2O2、4-氨基安替比林和酚生成红色醌类化合物,在500nm有吸收峰,其颜色深浅与FC含量成正比。
自备仪器和用品:水浴锅、可调式移液枪、可见分光光度计/酶标仪、微量石英比色皿/96孔板、和蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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2023-08-10 11:44:41