柠檬酸合酶(CS)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Citrate Synthase(CS) Activity Assay Kit
储存条件:Store at -20℃,6 months


产品简介
中文名称 | 柠檬酸合酶(CS)活性检测试剂盒 |
英文名称 | Citrate Synthase(CS) Activity Assay Kit |
别名 | 柠檬酸合酶试剂盒 CS Kit 柠檬酸合酶(CS)试剂盒 柠檬酸合酶(CS)测试盒,生化法试剂盒 |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at -20℃,6 months |
规格 | 100T/48S |
单位 | 盒 |
测定意义:
CS(EC 2.3.3.1)广泛存在于动物、植物、微生物和培养细胞的线粒体基质中,是三羧酸循环第一个限速酶,是三羧酸循环主要调控位点之一。
测定原理:
CS催化乙酰CoA和草酰乙酸产生柠檬酰辅酶A,进一步水解产生柠檬酸;该反应促使无色的DTNB转变成黄色的TNB,在 412nm处有特征吸光值。
需要的仪器,耗材:
可见分光光度计/酶标仪、台式离心机、水浴锅、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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