6-磷酸葡萄糖脱氢酶(G6PDH)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Glucose-6-Phosphate Dehydrogenase(G6PDH)Activity Assay Kit
储存条件:Store at 2-8℃,6 months


产品简介
中文名称 | 6-磷酸葡萄糖脱氢酶(G6PDH)活性检测试剂盒 |
英文名称 | Glucose-6-Phosphate Dehydrogenase(G6PDH)Activity Assay Kit |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at 2-8℃,6 months |
推荐 | 若使用96孔板测定,需使用96孔UV板,推荐货号YA0602 |
规格 | 100T/96S |
单位 | 盒 |
测定意义:G6PDH(EC 1.1.1.49)广泛存在于动物、植物、微生物和培养细胞中,是磷酸戊糖途径的关键酶,催化 6-磷酸葡萄糖氧化为 6 -磷酸葡萄糖酸内酯,同时将NADP+还原为NADPH,供生物合成及维持细胞内的还原状态用。因此6-磷酸葡萄糖脱氢酶活性的高低可以从一定程度上反映出生物体的生物合成和抗氧化能力。
测定原理:G6PDH催化NADP+还原生成NADPH,在340 nm下测定NADPH增加速率。
需要的仪器,耗材:紫外分光光度计/酶标仪、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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