基因名 |
MGEA5 |
别名 |
Bifunctional protein NCOAT;Glycoside hydrolase O-GlcNAcase;HAT;HEXC;Hexosaminidase C;Histone acetyltransferase;KIAA0679;MEA5;Meningioma expressed antigen 5;Meningioma-expressed antigen 5;MGEA 5;N-acetyl-beta-D-glucosaminidase;N-acetyl-beta-glucosaminidase;NCOAT;Nuclear cytoplasmic O GlcNAcase and acetyltransferase;Nuclear cytoplasmic O-GlcNAcase and acetyltransferase;O-GlcNAcase;OGA;NCOAT_HUMAN |
英文名称 |
Recombinant human MGEA5 protein |
全名 |
meningioma expressed antigen 5 (hyaluronidase) |
中文名称 |
组蛋白乙酰转移酶(脑膜瘤表达抗原5) |
外观(性状) |
Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. (Or liquid with 5% glycerol.) |
纯度 |
≥85% by SDS-PAGE. |
储存条件 |
Reconstituted protein solution can be stored at 4-7°C for 1-2 weeks, stored at < -20°C for 1 year. |
运输条件 |
冷藏运输 |
浓度 |
1mg/ml by SDS-PAGE. |
Swiss Prot |
O60502-2 |
Gene ID |
10724 |
交叉反应 |
Human Mouse Rat |
分子量 |
39kDa |
区间范围 |
351-700aa |
背景说明 |
Isoform 1:Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210).Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).Isoform 3:Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. |
应用 |
Immunology research |
来源 |
E.coli |
纯化方法 |
NI-NTA affinity purification |
溶剂 |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
内毒素 |
Not measured |
生物活性 |
NO |
标签信息 |
With a 6×His tag at the N/C-terminus. |
规格 |
10ug 50ug 100ug 1mg |
单位 |
支 |
属性分类 |
重组抗原 |