| 基因名 |
LRRC8E |
| 别名 |
Volume-regulated anion channel subunit LRRC8E;Leucine-rich repeat-containing protein 8E |
| 英文名称 |
Recombinant human LRRC8E protein |
| 全名 |
leucine rich repeat containing 8 family，member E |
| 中文名称 |
富含亮氨酸重复序列蛋白8家族成员E |
| 外观(性状) |
Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. (Or liquid with 5% glycerol.) |
| 纯度 |
≥85% by SDS-PAGE. |
| 储存条件 |
Reconstituted protein solution can be stored at 4-7°C for 1-2 weeks, stored at < -20°C for 1 year. |
| 运输条件 |
冷藏运输 |
| 浓度 |
1mg/ml by SDS-PAGE. |
| Swiss Prot |
Q6NSJ5 |
| Gene ID |
80131 |
| 交叉反应 |
Human Mouse Rat |
| 分子量 |
50.2kDa |
| 区间范围 |
353-796aa |
| 背景说明 |
Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24790029, PubMed:26824658, PubMed:28193731).The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed:24790029, PubMed:26824658).Mediates efflux of amino acids, such as aspartate, in response to osmotic stress (PubMed:28193731).The VRAC channel also mediates transport of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol (PubMed:33171122).Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24790029, PubMed:26824658, PubMed:28193731).Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition: lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis (PubMed:33139539). |
| 应用 |
Immunology research |
| 来源 |
E.coli |
| 纯化方法 |
NI-NTA affinity purification |
| 溶剂 |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 内毒素 |
Not measured |
| 生物活性 |
NO |
| 标签信息 |
With a 6×His tag at the N/C-terminus. |
| 规格 |
10ug 50ug 100ug 1mg |
| 单位 |
支 |
| 属性分类 |
重组抗原 |
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