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增强型DAB显色试剂盒（20×）
英文名称:Metal Enhanced DAB Substrate Kit，20×
储存条件:2-8℃，avoid light，1 year
产品简介
| 英文名称 | Metal Enhanced DAB Substrate Kit，20× |
| 中文名称 | 增强型DAB显色试剂盒（20×） |
| 储存条件 | 2-8℃，avoid light，1 year |
| 规格 | 60ml 200ml |
| 单位 | 盒 |
产品简介：
增强型DAB显色试剂盒是一种借助辣根过氧化物酶(HRP)反应，可用于免疫组化显色、原位杂交显色或Western、Southern 、Northern、EMSA等膜显色的试剂盒。DAB是辣根过氧化物酶的常用底物。在辣根过氧化物酶的催化下，DAB会产生棕色沉淀。该棕色沉淀不溶于水和乙醇，可用于正常脱水封片。
本产品采用特殊配方，灵敏度高，背景低，重复性好，储存稳定，使用方便，适合于蛋白印迹、免疫组织化学和免疫细胞化学、斑点印迹和生物芯片等的染色和显色反应。
操作说明（仅供参考）：
1、 对于组织切片或蛋白质印记膜，在与辣根过氧化物酶（HRP）标记的抗体或其它形式的探针孵育后，用适当洗涤液洗涤3-5次，每次3-5分钟。（见注意事项1）
2、 将溶液 A、B、C按照1:1:18的比例混合均匀，即为DAB工作液，建议4h内使用。（见注意事项2）
3、 向组织切片或膜上加入适量DAB显色工作液，确保能充分覆盖样品。（见注意事项3）
4、 室温避光孵育1-30 分钟，显色时间过长可引起本底增高，故应密切观察显色过程（一般3-10分钟最理想），并在本底较浅且达到适当显色强度时以流水漂洗终止显色反应。
5、 对于组织切片或细胞样品，显色反应终止后可对其进行其他染料（常用苏木素染色液）复染。对于膜，显色反应终止后，可以室温晾干避光保存。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Publish_to：
Journal of Cancer
PMID：
39668819
Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection
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Author：
Zhang Mei, Du Yanhua, Yang Li, Zhan Lin, Yang Bin, Huang Xueyong, Xu Bianli, Morita Koichi, Yu Fuxun
IF：
3.2570
Publish_to：
Brazilian Journal of Infectious Diseases
PMID：
35835158
Therapeutic effects of kartogenin on temporomandibular joint injury by activating the TGF-β/SMAD pathway in rats
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Author：
Chengzhi Zhao, Li Ye, Zhiwei Cao, Xing Tan, Yubin Cao, Jian Pan
IF：
3.2000
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EXPERIMENTAL BIOLOGY AND MEDICINE
PMID：
36941805
Environmental carbon tetrachloride exposure disrupts the liver structure and metabolic detoxification function in mice via p38MAPK/ NF-κB/NLRP3 pathway
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Author：
魏媛媛
IF：
3.1010
Publish_to：
Food and Agricultural Immunology
PMID：
MiR-135a-5p regulates window of implantation by suppressing pinopodes development and decidualization of endometrial stromal cells
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Author：
He Yunan, Ju Ying, Lei Hui, Dong Jie, Jin Ni, Lu Jie, Chen Shuqiang, Wang Xiaohong
IF：
3.1000
Publish_to：
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
PMID：
38512656
METTL3 suppressing SLC31A1 m6A modification regulates trophoblast migration and invasion
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Author：
Song Wang, Yixiong Lin, Qiong Deng, Xinyang Shen, Qian Chen, Xiaojing Yue, Zhijian Wang
IF：
3.0000
Publish_to：
PLACENTA
PMID：
40088504
Simultaneous blastic plasmacytoid dendritic cell neoplasm and myelofibrosis: A case report
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Author：
Fuyi Luo, Bingjie Li, Jing Li, Yan Li
IF：
2.9000
Publish_to：
Oncology Letters
PMID：
38586204
Natural emodin reduces myocardial ischemia/reperfusion injury by modulating the RUNX1/miR‑142‑3p/DRD2 pathway and attenuating inflammation
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Author：
Xuezhi Zhang, Qiaoji Qin, Xianghong Lv, Yongbin Wang, Feng Luo, Li Xue
IF：
2.7510
Publish_to：
Experimental and Therapeutic Medicine
PMID：
36561980
Experimental study of a 3D-printing technique combined with biphasic calcium phosphates to treat osteonecrosis of the femoral head in a canine model
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Author：
Chen Zhian, Feng Fanzhe, Su Xixiong, Xu Yongqing, Zhang Ying, Tan Hongbo
IF：
2.6000
Publish_to：
Journal of Orthopaedic Surgery and Research
PMID：
37716998
Basic fibroblast growth factor protects against liver ischemia-reperfusion injury via the Nrf2/Hippo signaling pathway
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Author：
Xixi Chen, Gaozan Tong, Saizhen Chen
IF：
2.5860
Publish_to：
TISSUE & CELL
PMID：
36150335
SDF-1/CXCR4 axis participants in the pathophysiology of adult patients with moyamoya disease
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Author：
Shuaiyu Ren, Qingdong Han, Peng Zhou, Zongqi Wang, Yabo Huang
IF：
2.5000
Publish_to：
Journal of Stroke & Cerebrovascular Diseases
PMID：
38608825
Salvianolic acid B inhibits the progression of liver fibrosis in rats via modulation of the Hedgehog signaling pathway
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Author：
Shanjun Tao, Renjie Duan, Tong Xu, Jiao Hong, Wenjie Gu, Aiqin Lin, Likai Lian, Haoyu Huang, Jiangtao Lu, Tiechen Li
IF：
2.4470
Publish_to：
Experimental and Therapeutic Medicine
PMID：
34970339
PF4 inhibits ferroptosis-mediated intracerebral hemorrhage through modulating the CXCR3/AKT1/SLC7A11 signaling pathway
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Author：
Na Hu, Guohong Zhang, Liping An, Wei Wang, Ran An, Yunfeng Li
IF：
2.2000
Publish_to：
Biomolecules and Biomedicine
PMID：
39558855
Preliminary evaluation of the protective effect of rEi-SAG19 on Eimeria intestinalis infection in rabbits
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Author：
Jiayan Pu, Ge Hao, Hao Chen, Wei He, Changming Xiong, Jie Xiao, Guangyou Yang
IF：
1.4000
Publish_to：
EXPERIMENTAL PARASITOLOGY
PMID：
39423994
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