| 基因名 |
APEX2 |
| 别名 |
AP endonuclease XTH2；APEX nuclease 2；APEX nuclease-like 2；AP endonuclease 2 |
| 英文名称 |
Recombinant human APEX2 protein |
| 全名 |
APEX nuclease (apurinic/apyrimidinic endonuclease) 2 |
| 中文名称 |
APEX核酸酶2 |
| 外观(性状) |
Powder: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. (Or liquid with 5% glycerol.) |
| 纯度 |
≥85% by SDS-PAGE. |
| 储存条件 |
Reconstituted protein solution can be stored at 4-7°C for 2-7 days, stored at < -20°C for 1 year. |
| 运输条件 |
The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 浓度 |
1mg/ml by SDS-PAGE. |
| Swiss Prot |
Q9UBZ4 |
| Gene ID |
27301 |
| 交叉反应 |
Human Mouse (Bovine) |
| 分子量 |
39kDa |
| 区间范围 |
172-518aa |
| 背景说明 |
Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents.Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities.Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially.Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents.In the nucleus functions in the PCNA-dependent BER pathway.Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes.Required for proper cell cycle progression during proliferation of peripheral lymphocytes. |
| 应用 |
Immunology research |
| 来源 |
E.coli |
| 纯化方法 |
NI-NTA affinity purification |
| 溶剂 |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 10μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 内毒素 |
Not measured |
| 生物活性 |
No activity experimental data, to be verified. |
| 标签信息 |
With a 6×His tag at the N/C-terminus. |
| 规格 |
10ug 50ug 100ug 1mg |
| 单位 |
支 |
| 属性分类 |
重组抗原 |
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