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异硫氰酸荧光素酯 FITC
英文名称:FITC
CAS:No.3326-32-7
分子式:C21H11NO5S
分子量:389.38
储存条件:-20℃保存，有效期1年。
产品简介
| 中文名称 | 异硫氰酸荧光素酯 FITC |
| 英文名称 | FITC |
| CAS | No.3326-32-7 |
| 分子式 | C21H11NO5S |
| 分子量 | 389.38 |
| 纯度/级别 | ≥95%/Conjugation Grade |
| 外观(性状) | 橘黄色粉末 |
| 溶解性 | 5mg/ml DMSO |
| 储存条件 | -20℃保存，有效期1年。 |
| 危险代码 | R：42/43 S：22-36/37-45 |
| 规格 | 50mg |
| 单位 | 瓶 |
异硫氰酸荧光素酯是黄色粉末。有吸湿性。能与各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中仍有强烈绿色荧光，加酸后析出沉淀，荧光消失，微溶于丙酮、乙醚和石油醚。
异硫氰酸荧光素酯是生化试剂,也是医学诊断药物,主要用于荧光抗体技术中的荧光染料,能和各种抗体蛋白结合,结合后的抗体不丧失与一定抗原结合的特异性,并在碱性溶液中具有强烈的绿色荧光。用于医学,农学和畜牧等方面,可对由地细菌病毒和寄生虫等所致疾病进行快速诊断。
备注：产品信息可能会有优化升级。请以实际标签信息为准。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Shaoqi Qu, Yiming Han, Ying Liu, Jiajia Zhu, Ulas Acaroz, Jianzhong Shen, Kui Zhu
IF：
5.8950
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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36515136
Multicomponent thermosensitive lipid complexes enhance desmoplastic tumor therapy through boosting anti-angiogenesis and synergistic strategy
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Jian Guo, Huating Zeng, Yimin Liu, Xinmeng Shi, Yuping Liu, Congyan Liu, Yan Chen
IF：
5.8750
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
PMID：
33781886
Effect of Strontium-Substituted Calcium Phosphate Coatings Prepared by One-Step Electrodeposition at Different Temperatures on Corrosion Resistance and Biocompatibility of AZ31 Magnesium Alloys
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Yingchao Xu, Guangyu Li, Zhihui Zhang, Jianshe Lian, Yunting Guo, Luquan Ren
IF：
5.8000
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ACS Biomaterials Science & Engineering
PMID：
38147691
Flower-like Composite Material Delivery of Co-Packaged Lenvatinib and Bufalin Prevents the Migration and Invasion of Cholangiocarcinoma
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Zhouyu Ning, Yingke Zhao, Xia Yan, Yongqiang Hua, Zhiqiang Meng
IF：
5.7190
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Nanomaterials
PMID：
35745387
Pyroptosis, apoptosis, and autophagy are involved in infection induced by two clinical Klebsiella pneumoniae isolates with different virulence
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Author：
Wang Xueting, Bi Chunxia, Xin Xiaoni, Zhang Mengmeng, Fu Hengxia, Lan Lei, Wang Mengyuan, Yan Zhiyong
IF：
5.7000
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Frontiers in Cellular and Infection Microbiology
PMID：
37223846
Zinc Oxide Nanoparticles Prime a Protective Immune Response in Galleria mellonella to Defend Against Candida albicans
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Xu Mei-nian, Li Li, Pan Wen, Zheng Huan-xin, Wang Meng-lei, Peng Xiao-ming, Dai Si-qi, Tang Ying-mei, Zeng Kang, Huang Xiao-wen
IF：
5.6400
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Frontiers in Microbiology
PMID：
34956129
Efficiency of a novel thermosensitive enema in situ hydrogel carrying Periplaneta americana extracts for the treatment of ulcerative colitis
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Author：
Ming Wu, Hui Ding, Xiao Tang, Jiayi Chen, Meng Zhang, Ziqiong Yang, Qian Du, Jun Wang
IF：
5.6000
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Frontiers in Pharmacology
PMID：
36843930
Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains
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Qianqian Lu, Yingqi Guo, Guo Yang, Lei Cui, Zhen Wu, Xiaoqun Zeng, Daodong Pan, Zhendong Cai
IF：
5.5610
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Foods
PMID：
35681360
Development and characterization of MAO/PLA-nHA nanocomposite coatings on pure zinc for orthopedic applications
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Author：
Quanxin Chen, Xinglong Zhu, Yongqi Jiang, Lijing Yang, Huinan Hannah Liu, Zhenlun Song
IF：
5.4000
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SURFACE & COATINGS TECHNOLOGY
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Novel double-layered PLGA microparticles-dissolving microneedle (MPs-DMN) system for peptide drugs sustained release by transdermal delivery
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Author：
Bo Xu, Han Liu, Guozhong Yang, Suohui Zhang, Zequan Zhou, Yunhua Gao
IF：
5.3000
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
PMID：
39722375
Properties of Heat-Assisted pH Shifting and Compounded Chitosan from Insoluble Rice Peptide Precipitate and Its Application in the Curcumin-Loaded Pickering Emulsions
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Zhenyu Yang, Zhiying Li, Zitong Xu, Zhihao Kong, Xin Qiao, Liwen Zhang, Lei Dai, Yanfei Wang, Qingjie Sun, David Julian McClements, Xingfeng Xu
IF：
5.2000
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Foods
PMID：
38137189
CRP Involved in Nile Tilapia (Oreochromis niloticus) against Bacterial Infection
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Qi Li, Baijian Jiang, Zhiqiang Zhang, Yongxiong Huang, Zhou Xu, Xinjin Chen, Jia Cai, Yu Huang, Jichang Jian
IF：
5.1680
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Biology-Basel
PMID：
36009776
Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I
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Author：
Ge Liu, Rui Liu, Yeqi Shan, Chaomin Sun
IF：
5.1570
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JOURNAL OF BIOLOGICAL CHEMISTRY
PMID：
34461092
Four novel anti-adhesive activity peptides against Helicobacter pylori derived from rice bran protein: release, identification and anti-adhesive mechanisms elucidation
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Guanlong Li, Zhengfei Miao, Xiaolan Liu, QuanXin Wang, Xiqun Zheng
IF：
5.1000
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Food & Function
PMID：
39042096
EDTA-functionalized silica nanoparticles as a conditioning agent for dentin bonding using etch-and-rinse technique
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Jianan Yu, Yuexiang Li, Xinyuan Liu, Haowen Huang, Yu Wang, Qunlin Zhang, Quanli Li, Chris Ying Cao
IF：
4.9910
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JOURNAL OF DENTISTRY
PMID：
37105434
A new self-immolative colistin prodrug with dual targeting functionalities and reduced toxicity for the treatment of intracellular bacterial infections
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Gengqi Liu, Di Lu, Shiyu Zhu, Minchao Hao, Xingyue Yang, Xiaojian Wang, Yumiao Zhang
IF：
4.8540
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
PMID：
35593460
Molecular characterization and functional analysis of galectin-1 from silver pomfret (Pampus argenteus)
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Suyang Chen, Tingting Gao, Xionglin Li, Kejing Huang, Lu Yuan, Suming Zhou, Jianhu Jiang, Yajun Wang, Jiasong Xie
IF：
4.7000
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FISH & SHELLFISH IMMUNOLOGY
PMID：
37944682
CpG ODN 2102 promotes antibacterial immune responses and enhances vaccine-induced protection in golden pompano (Trachinotus ovatus)
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Xiaojuan Chen, Ying Wu, Yulin Qiu, Pengshuo Li, Zhenjie Cao, Yongcan Zhou, Yun Sun
IF：
4.6220
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FISH & SHELLFISH IMMUNOLOGY
PMID：
37137380
Identification and characterization of a C-type lectin in turbot (Scophthalmus maximus) which functioning as a pattern recognition receptor that binds and agglutinates various bacteria
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Qing Zhu, Huijun Huo, Qiang Fu, Ning Yang, Ting Xue, Changju Zhuang, Xiaoli Liu, Beibei Wang, Baofeng Su, Chao Li
IF：
4.5810
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FISH & SHELLFISH IMMUNOLOGY
PMID：
34062237
Toxicities of polystyrene microplastics (MPs) and hexabromocyclododecane (HBCD), alone or in combination, to the hepatopancreas of the whiteleg shrimp, Litopenaeus vannamei
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Author：
Jingyu Liu, Yifan Wang, Yanqin Liang, Shengli Zhu, Hui Jiang, Shuilin Wu, Xiang Ge, Zhaoyang Li
IF：
4.5000
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Biomimetics
PMID：
37366857
The chiisanoside derivatives present in the leaves of Acanthopanax sessiliflorus activate autophagy through the LRP6/GSK3β axis and thereafter inhibit oxidative stress, thereby counteracting cisplatin-induced ototoxicity
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Wenxin Zhang, Hongbo Teng, Tianyi Zhao, Roberts I. Eglitis, Xv Wang, Zhengxuan Yu, Shurong Qu, Haijing Wang, Yaru Zhao, Bowen Fan, Shuangli Liu, Yan Zhao
IF：
4.4000
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Frontiers in Pharmacology
PMID：
39881873
The Amelogenin-Derived Peptide TVH-19 Promotes Dentinal Tubule Occlusion and Mineralization
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Author：
Xiu Peng, Sili Han, Kun Wang, Longjiang Ding, Zhenqi Liu, Linglin Zhang
IF：
4.3290
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Polymers
PMID：
34372076
Tumoral EIF4EBP1 regulates the crosstalk between tumor-associated macrophages and tumor cells in MRTK
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Xin Wu, Tao Mi, Liming Jin, Chunnian Ren, Jinkui Wang, Zhaoxia Zhang, Jiayan Liu, Zhaoyin Wang, Peng Guo, Dawei He
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4.2000
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EUROPEAN JOURNAL OF PHARMACOLOGY
PMID：
38944176
Enhancement of immune responses using ovalbumin-conjugated Eucommia ulmoides leaf polysaccharides encapsulated in a cubic liquid-crystalline phase delivery system
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Haibo Feng, Linzi Zhang, Jie Yang, Sheng Li, Feng Tang, Hangyu Li, Xinnan Zhang, Daiyan Wu, Yangyang Feng, Qianqian Liu, Ziwei Liu
IF：
4.1250
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
35638143
Exogenous Amyloid Fibrils Can Cause Significant Upregulation of Neurodegenerative Disease Proteins
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Author：
Xihua Liu, Wenzhe Jia, Yapeng Fang, Yiping Cao
IF：
4.1000
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ACS Chemical Neuroscience
PMID：
39424294
Discovery of Salifungin as a Repurposed Antibiotic against Methicillin-Resistant Staphylococcus aureus with Limited Resistance Development
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Chenchen Wang, Yueyue Ji, Xingyu Huo, Xiaodan Li, Wenjia Lu, Zhaoran Zhang, Wenqi Dong, Xiangru Wang, Huanchun Chen, Chen Tan
IF：
4.0000
Publish_to：
ACS Infectious Diseases
PMID：
38581387
Identification and response analysis of xyloglucan endotransglycosylase/hydrolases (XTH) family to fluoride and aluminum treatment in Camellia sinensis
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Wu Zichen, Cui Chuanlei, Xing Anqi, Xu Xiaohan, Sun Yi, Tian Zhiqiang, Li Xuyan, Zhu Jiangyuan, Wang Genmei, Wang Yuhua
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3.9690
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BMC GENOMICS
PMID：
34696727
GelMA/κ-carrageenan double-network hydrogels with superior mechanics and biocompatibility
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Author：
Xueqi Gan, Chen Li, Jiyu Sun, Xidan Zhang, Min Zhou, Yi Deng, Anqi Xiao
IF：
3.9000
Publish_to：
RSC Advances
PMID：
36688070
3D MXene microspheres with honeycomb architecture for tumor photothermal/photodynamic/chemo combination therapy
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Author：
Yaping Guo, Hongshui Wang, Xin Feng, Yunbo Zhao, Chunyong Liang, Lei Yang, Mingjun Li, Yongguang Zhang, Wei Gao
IF：
3.8740
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NANOTECHNOLOGY
PMID：
33513589
Effect of microencapsulated Fiber2-displaying probiotics loaded with inulin nanoparticles on immunity against fowl adenovirus serotype 4 in chickens
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Author：
Biao Wang, Meiyuan Tian, Yashu Yang, Zhipeng Jia, Faisal R Anjum, Chunli Ma, Dexing Ma
IF：
3.8000
Publish_to：
POULTRY SCIENCE
PMID：
39778367
MXene Quantum Dot/Zeolitic Imidazolate Framework Nanocarriers for Dual Stimulus Triggered Tumor Chemo-Phototherapy
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Xin Feng, Mingjun Li, Jianming Wang, Xianrui Zou, Hongshui Wang, Donghui Wang, Huan Zhou, Lei Yang, Wei Gao, Chunyong Liang
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3.7480
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Materials
PMID：
35806667
Pseudorabies virus infection increases the permeability of the mammalian respiratory barrier to facilitate Pasteurella multocida infection
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Dajun Zhang, Lin Lin, Jie Yang, Qingjie Lv, Mixue Wang, Lin Hua, Keshan Zhang, Huanchun Chen, Bin Wu, Zhong Peng
IF：
3.7000
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mSphere
PMID：
39041808
Molecular characterization and antibacterial ability of galectin-3 and galectin-9 in Onychostoma macrolepis
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Author：
Hongzhou Xu, Haixia Liu, Chengrong Liu, Xinyan Shangguan, Xu Cheng, Ruifang Zhang, Yitong Lu, Ping Li, Yingjie Cai
IF：
3.6360
Publish_to：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
PMID：
34914929
pH-responsive black phosphorus quantum dots for tumor-targeted photodynamic therapy
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Zhaoyuan Liu, Zhongjian Xie, Xinqiang Wu, Zijian Chen, Wenting Li, Xiaofeng Jiang, Liangqi Cao, Dawei Zhang, Qiwen Wang, Ping Xue, Han Zhang
IF：
3.6310
Publish_to：
Photodiagnosis and Photodynamic Therapy
PMID：
34237475
IVT cell-free biosensors for tetracycline and macrolide detection based on allosteric transcription factors (aTFs)
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Huaixiu Bi, Chen Zhao, Yongkang Zhang, Xi Zhang, Bin Xue, Chenyu Li, Shang Wang, Xiaobo Yang, Chao Li, Zhigang Qiu, Jingfeng Wang, Zhiqiang Shen
IF：
3.5320
Publish_to：
Analytical Methods
PMID：
36314439
Biomimetic Inorganic Nanoparticle-Loaded Silk Fibroin-Based Coating with Enhanced Antibacterial and Osteogenic Abilities
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Author：
Yunpeng Zhang, Xiaorong Chen, Yuan Li, Tian Bai, Chen Li, Lingyan Jiang, Yu Liu, Changying Sun, Wenhao Zhou
IF：
3.5120
Publish_to：
ACS Omega
PMID：
34778674
The role of a novel secretory peptidoglycan recognition protein with antibacterial ability from the Chinese Oak Silkworm Antheraea pernyi in humoral immunity
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Xutong Duan, Ting Fu, Chang Liu, Fuhui Wang, Chengbao Liu, Lin Zhao, JinZhu Yu, Xialu Wang, Rong Zhang
IF：
3.2000
Publish_to：
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
PMID：
38880307
Gallic acid has anticancer activity and enhances the anticancer effects of cisplatin in non small cell lung cancer A549 cells via the JAK/STAT3 signaling pathway
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Tingxiu Zhang Lijie Ma Pengfei Wu Wei Li T
IF：
2.9760
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Oncology Reports Pages: 1779-1788
PMID：
30747218
Inhibitory role of microRNA-484 in kidney stone formation by repressing calcium oxalate crystallization via a VDR/FoxO1 regulator axis
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Fan Li, Li Hai, Huo Wei
IF：
2.8610
Publish_to：
Urolithiasis
PMID：
36227295
Nerve growth factor loaded macrophage-derived nanovesicles for inhibiting neuronal apoptosis after spinal cord injury
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Author：
夏楠
IF：
2.6460
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Journal of Biomaterials Applications
PMID：
34167336
Assessment of probiotic adhesion and inhibitory effect on Escherichia coli and Salmonella adhesion
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Wang Ya, Jia Dan, Wang Jia-hui, Li He-hai, Liu Jun-long, Liu Ai-hong, Wang Jin-ming, Guan Gui-quan, Luo Jian-xun, Yin Hong, Li You-quan
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2.5520
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ARCHIVES OF MICROBIOLOGY
PMID：
34581832
Lactobacillus animalis pZL8a: a potential probiotic isolated from pig feces for further research
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Jia Dan, Wang Ya, Wang Jiahui, Liu Junlong, Li Hehai, Liu Aihong, Wang Jinming, Guan Guiquan, Luo Jianxun, Yin Hong, Li Youquan
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2.4060
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3 Biotech
PMID：
33680697
Bacterial coloration immunofluorescence strip for ultrasensitive rapid detection of bacterial antibodies and targeted antibody-secreting hybridomas
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Shuiqin Fang, Hao Yang, Cheng Liu, Yachen Tian, Meijiao Wu, Youxue Wu, Qing Liu
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2.3030
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JOURNAL OF IMMUNOLOGICAL METHODS
PMID：
34933017
Protocol for rapid evaluation of antibody-mediated protection in macrophages using immunofluorescence techniques
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Author：
Lingyuan Zeng, Hao Li
IF：
1.3000
Publish_to：
STAR Protocols
PMID：
39302838
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异硫氰酸荧光素和异硫氰酸荧光素酯在标记细菌上的区别是什么2025-03-01 14:19:30
您好，异硫氰酸荧光素（FITC）和异硫氰酸荧光素酯在标记细菌上有以下一些区别：1. 化学结构与性质，FITC：具有相对较小的分子结构，含有异硫氰酸基，能与生物分子中的氨基等基团发生特异性反应，在碱性条件下，异硫氰酸基可与细菌表面的氨基形成稳定的硫脲键，从而实现对细菌的标记。它的水溶性较好，能在水溶液中稳定存在，有利于在生物体系中的应用。异硫氰酸荧光素酯：通常是 FITC 的酯类衍生物，其分子结构中酯基的存在使其化学性质与 FITC 有所不同。酯基在某些条件下可发生水解反应，生成相应的酸和醇。在标记细菌时，酯基可能需要先经过水解等过程，释放出具有活性的异硫氰酸荧光素部分，才能与细菌表面的基团发生反应。其水溶性一般较 FITC 差，可能需要使用有机溶剂助溶，但在一些非水相或疏水性环境中可能具有更好的溶解性和稳定性。2. 标记反应条件，FITC：标记细菌时，通常在温和的碱性条件下进行，例如 pH 值在 7 - 9 之间。反应温度一般在 4 - 37℃，反应时间根据具体细菌种类和实验要求有所不同，一般在 30 分钟至数小时不等。由于 FITC 水溶性好，可直接溶解在缓冲液中与细菌混合进行标记，操作相对简便。异硫氰酸荧光素酯：由于其酯基的存在，标记反应可能需要更复杂的条件。在一些情况下，需要先将其溶解在适当的有机溶剂中，然后再加入到含有细菌的缓冲液中。同时，可能需要考虑酯基水解的条件，例如加入适量的酯酶或控制反应体系的 pH 值和温度，以促进酯基水解并使释放出的 FITC 与细菌发生标记反应。反应条件的优化可能更为关键，以确保酯基能够有效地水解并实现高效的细菌标记。3. 标记效果与应用，FITC：标记细菌后，由于其分子结构相对较小，能够较为均匀地分布在细菌表面，对细菌的生理功能和形态影响较小。标记后的荧光信号稳定，荧光强度较高，有利于通过荧光显微镜、流式细胞仪等设备进行检测和分析。广泛应用于细菌的定位、计数、动态监测以及细菌与其他生物分子相互作用等研究领域。异硫氰酸荧光素酯：标记细菌后，其荧光性质与 FITC 类似，但由于酯基水解过程的存在，可能在标记效率和荧光信号的稳定性方面与 FITC 有所差异。在某些特定的应用中，例如研究细菌在疏水性环境中的行为或与脂质体等疏水性物质的相互作用时，异硫氰酸荧光素酯可能具有独特的优势。此外，由于其化学性质的差异，在一些需要对标记过程进行特殊控制或对标记产物进行特定修饰的实验中，也可能会选择异硫氰酸荧光素酯。
溶解后可以保存多长时间2025-01-09 21:12:36
您好，如果是溶于有机溶剂配置成高浓度储备液，置于-20℃避光保存通常可以保存至少3个月，如果是工作浓度，一般置于4℃避光可以保存2-3天。
请问FITC的激发波长和发射波长是多少？2025-01-07 14:27:31
您好，FITC的激发波长通常在488nm左右，发射波长通常在520nm左右
请问FITC可以标记RNA吗2024-09-21 21:43:04
您好，FITC（异硫氰酸荧光素）可以标记 RNA，其标记原理基于 FITC 的异硫氰酸酯基团（-N=C=S）与 RNA 分子中的氨基（-NH?）发生化学反应，形成稳定的硫脲键。不过，天然 RNA 分子通常不含游离氨基，因此需要通过以下方法对 RNA 进行预处理，引入氨基后再与 FITC 偶联。
请问FITC可以用于标记细菌么2024-08-09 09:43:36
您好，FITC（异硫氰酸荧光素）可以用于标记细菌，其原理是通过化学反应与细菌表面或内部的氨基（-NH?）结合，从而实现荧光标记。