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线粒体呼吸链复合体Ⅴ/ATP合酶活性检测试剂盒
检测方法:可见分光光度法可见分光光度法 Spectrophotometer
英文名称:Mitochondrial Complex V/ATP Synthase Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 线粒体呼吸链复合体Ⅴ活性检测试剂盒 |
| 英文名称 | Mitochondrial Complex V/ATP Synthase Activity Assay Kit |
| 检测方法 | 可见分光光度法可见分光光度法 Spectrophotometer |
| 储存条件 | Store at -20℃，6 months |
| 规格 | 25T/12S 50T/24S |
| 单位 | 盒 |
测定意义：线粒体复合体Ⅴ又称F1F0-ATP合酶，广泛存在于动物、植物、微生物和培养细胞的线粒体中，由F1和F0两个亚单位组成。该酶利用呼吸链产生的质子电化学梯度催化ATP合成，也可逆过程水解ATP。此外，复合体Ⅴ还存在于叶绿体、异养菌和光合细菌中。复合体Ⅴ是线粒体氧化磷酸化和叶绿体光合磷酸化合成ATP的关键酶。
测定原理：复合体Ⅴ水解ATP 产生ADP 和Pi，通过测定Pi 增加速率来测定复合体Ⅴ活性。
试验中所需的仪器和试剂：紫外分光光度计、台式离心机、水浴锅、可调式移液器、1mL玻璃比色皿、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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