硫氧还蛋白还原酶(TrxR)活性检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit
储存条件:Store at -20℃,6 months


产品简介
自备试剂 | 该试剂盒实验过程中需自备试剂,详情见网站说明书 |
中文名称 | 硫氧还蛋白还原酶(TrxR)活性检测试剂盒 |
英文名称 | Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit |
检测方法 | 可见分光光度法 Spectrophotometer |
储存条件 | Store at -20℃,6 months |
规格 | 50T/48S |
单位 | 盒 |
测定意义:TrxR 是一种NADPH 依赖的包含FAD 结构域的二聚体硒酶,属于吡啶核苷酸-二硫化物氧化还原酶家族成员,与硫氧还蛋白以及NADPH 共同构成了硫氧还蛋白系统。TrxR 与GR 活性类似,催化GSSG 还原生成GSH,是谷胱甘肽氧化还原循环关键酶之一。
测定原理:TrxR 催化NADPH 还原DTNB 生成TNB 和NADP+,TNB 在412 nm 有特征吸收峰,通过测定412nm 波长处TNB 的增加速率,即可计算TrxR 活性。
自备仪器和用品:可见分分光光度计、低温离心机、可调节移液器、1mL 玻璃比色皿和蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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