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磺基-Cy7 羧酸

CAS:No.943298-08-6

英文名称:CY7

分子式:C35H42N2O8S2

分子量:682.85

储存条件:Powder:-20℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:≥96%

磺基-Cy7 羧酸
满3999赠露营车
货号:
IC0740
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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使用本产品的案例(仅供参考)

In Vitro(标记蛋白,Cy7:重组蛋白=20:1,RT,4h;透析纯化去除 未标记上的Cy7;Cy7 标记好的蛋白(bFGF-Cy7/LET-bFGF-Cy7)可用于活体成像,静脉注射)

Briefly, bFGF and LET-bFGF were labeled with Cy7 (Solarbio, Beijing, China) by dissolving Cy7 in DMF and mixing with the recombinant protein [in phosphate-buffered saline (PBS)] at a 20:1 ratio while stirring at room temperature for 4 hours.   Resulting solutions were concentrated and purified by dialysis to remove uncoupled Cy7.

Twenty-four male Sprague–Dawley rats were randomized into four groups: normal plus bFGF, normal plus LET-bFGF, irradiation plus bFGF, irradiation plus LET-bFGF.      The rats in the irraidation group received whole thorax radiation with total radiation dose of 15Gy using an X-ray generator. The rats in the normal group didn’t receive thorax radiation. Next, bFGF-Cy7 and LET-bFGF-Cy7 (500 μL, 30 μM) were intravenously injected into rats immediately after the irradiation and allowed to circulate for 1 hour before imaging rats under the IVIS imaging system (Perkin Elmer, Waltham, MA) at 710 nm. Finally, the lung, heart, liver, spleen, and kidneys were dissected and imaged by the IVIS imaging system.

来源文献:Guan D, Mi J, Chen X, Wu Y, Yao Y, Wang L, Xiao Z, Zhao Y, Chen B, Dai J. Lung endothelial cell-targeted peptide-guided bFGF promotes the regeneration after radiation induced lung injury. Biomaterials. 2018 Nov;184:10-19. doi: 10.1016/j.biomaterials.2018.08.061. Epub 2018 Sep 4. PMID: 30195801.

备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

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Guan D, et al. Biomaterials. 2018 Nov;184:10-19.
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