中文
英语
产品简介
| 中文名称 | 普鲁士蓝染色试剂盒(细胞专用) |
| 英文名称 | Prussian Blue Iron Stain Kit(For Cells) |
| 储存条件 | RT，避光保存，有效期1年。 |
| 规格 | 2*20ml 2*50ml |
| 单位 | 盒 |
含铁血黄素(Hemosiderin)是一种血红蛋白源性色素，为金黄色或棕黄色颗粒，因其含铁，且为金黄色，故称为含铁血黄素。当红细胞被巨噬细胞吞噬后，在溶酶体酶的作用下，血红蛋白被分解为不含铁的橙色血质和含铁的含铁血黄素。
Perls普鲁士蓝反应(Prussian blue reaction)又称为含铁血黄素染色，即经过亚铁氰化钾和稀酸处理后可以产生蓝色，常见于吞噬细胞或间质内，其染色原理在于亚铁氰化钾溶液使三价铁离子从蛋白质中被稀盐酸分离出来，三价铁与亚铁氰化钾反应，生成一种不溶解的蓝色化合物即三价铁的亚铁氰化物。
Perls stain主要用于显示细胞中铁离子，常见于吞噬细胞内，可以很好地区分含铁血黄素与其他色素。该染色液稳定性好、可以长期保存、不易产生沉淀、应用范围广，可以进行复染。该试剂盒适用于细胞的Perls stain，但是其染色强度受游离铁离子含量影响，含量太低可能会导致无蓝色生成。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
小鼠脾脏石蜡切片
含铁血黄素着蓝色，细胞核着浅红色
含铁血黄素着蓝色，细胞核着浅红色
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Fangyuan Zhang, Ziyun Li, Ping Gao, Jiaxi Zou, Yuting Cui, Yi Qian, Renjun Gu, Weiming Xu, Jingqing Hu
IF：
5.9880
Publish_to：
Frontiers in Pharmacology
PMID：
36483736
Iron Released after Cryo-Thermal Therapy Induced M1 Macrophage Polarization, Promoting the Differentiation of CD4+ T Cells into CTLs
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Author：
Shicheng Wang, Man Cheng, Peng Peng, Yue Lou, Aili Zhang, Ping Liu
IF：
5.9240
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
34209797
Magnetic resonance imaging tracing of superparamagnetic iron oxide nanoparticle–labeled mesenchymal stromal cells for repairing spinal cord injury
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Author：
Mai Xiaoli, Xie Yuanyuan, Wu Zhichong, Zou Junting, Du Jiacheng, Shen Yunpeng, Liu Hao, Chen Bo, Zhu Mengxia, Shi Jiong, Chen Yang, Zhang Bing, Zhu Zezhang, Wang Bin, Gu Ning
IF：
5.9000
Publish_to：
Neural Regeneration Research
PMID：
39435625
Synthesis and Photothermal Effects of Intracellular Aggregating Nanodrugs Targeting Nasopharyngeal Carcinoma
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Author：
Zhong Ying, Bejjanki Naveen Kumar, Miao Xiangwan, Weng Huanhuan, Li Quanming, Zhang Juan, Liu Tao, Vannam Raghu, Xie Minqiang
IF：
5.8900
Publish_to：
Frontiers in Bioengineering and Biotechnology
PMID：
34604188
Estrogen deficiency accelerates postmenopausal atherosclerosis by inducing endothelial cell ferroptosis through inhibiting NRF2/GPX4 pathway
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Author：
Ying Lv, Shan Zhang, Xiuzhu Weng, Jianxin Huang, Honggang Zhao, Xinyu Dai, Xiaoxuan Bai, Xiaoyi Bao, Chen Zhao, Ming Zeng, Yunshu Bai, Sining Hu, Ji Li, Haibo Jia, Bo Yu
IF：
5.8340
Publish_to：
FASEB JOURNAL
PMID：
37219513
Annexin A5 ameliorates traumatic brain injury-induced neuroinflammation and neuronal ferroptosis by modulating the NF-ĸB/HMGB1 and Nrf2/HO-1 pathways
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Author：
Yalong Gao, Hejun Zhang, Jiwei Wang, Fanjian Li, Xiaotian Li, Tuo Li, Cong Wang, Lei Li, Ruilong Peng, Li Liu, Weiyun Cui, Shu Zhang, Jianning Zhang
IF：
5.7140
Publish_to：
INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：
36700781
Single-cell sequencing decodes the secrets of the RAP phenomenon of corticotomy
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Author：
Fan Zhibo, Li Shenghong, You Liping, Lan Yuxin, Zhong Yutong, Ma Yuefan, Xu Jie, Xu Xiaomei
IF：
5.7000
Publish_to：
Frontiers in Immunology
PMID：
39430747
Nuciferine protects against lipopolysaccharide-induced endometritis via inhibiting ferroptosis and modulating AMPK汐/mTOR/HIF-1汐 signaling axis
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Author：
Peng Jiang, Linxian Zhao, Ruixue Hu, Zongzhen Zhai, Jian Guo, Kai Zhang
IF：
5.6000
Publish_to：
INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：
37734199
Buddlejasaponin IVb ameliorates ferroptosis of dopaminergic neuron by suppressing IRP2-mediated iron overload in Parkinson's disease
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Author：
Qiang-Ming Li, Tong Xu, Xue-Qiang Zha, Xiao-Wen Feng, Feng-Yun Zhang, Jian-Ping Luo
IF：
5.4000
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
37717841
A new metal ion chelator attenuates human tau accumulation-induced neurodegeneration and memory deficits in mice
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Author：
Yue Zuo, Hui-ting Liu, Lai-biao Lin, Rui-zhu Yue, Huan-huan Liu, Hong-wei Wang, Lu Wang, Ruan-ling Hou, Wei-zhen Liu, Chang-zheng Li, Jian-Zhi Wang, Peng Li, Ya-ling Yin
IF：
5.3000
Publish_to：
EXPERIMENTAL NEUROLOGY
PMID：
38141802
Iron chelation suppresses secondary bleeding after intracerebral hemorrhage in angiotensin II-infused mice
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Author：
Jie Wang, Xiao-qin Tang, Min Xia, Cheng-cheng Li, Chao Guo, Hong-fei Ge, Yi Yin, Bo Wang, Wei-xiang Chen, Hua Feng
IF：
5.2430
Publish_to：
CNS Neuroscience & Therapeutics
PMID：
34346561
Effects of magnetically targeted iron oxide@polydopamine-labeled human umbilical cord mesenchymal stem cells in cerebral infarction in mice
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Author：
Jun Yan, Te Liu, Yang Li, Jun Zhang, Bo Shi, Fuqiang Zhang, Xuejia Hou, Xiaowen Zhang, Wanxing Cui, Jing Li, Hua Yao, Xiuying Li, Yufei Gao, Jinlan Jiang
IF：
5.2000
Publish_to：
Aging-US
PMID：
36812482
Magnet-targeted delivery of bone marrow-derived mesenchymal stem cells improves therapeutic efficacy following hypoxic-ischemic brain injury
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Author：
Chuang Sun, Ao-Dan Zhang, Hong-Hai Chen, Jie Bian, Zheng-Juan Liu
IF：
5.1350
Publish_to：
Neural Regeneration Research
PMID：
33818519
Network Pharmacology Prediction and Experimental Verification for Anti-Ferroptosis of Edaravone After Experimental Intracerebral Hemorrhage
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Author：
Cao Suhan, Wei Jiating, Cai Yuangui, Xiong Zhiyi, Li Jianle, Jiang Zimu, Zhou Xueling, Huang Baozi, Zeng Jinsheng
IF：
5.1000
Publish_to：
MOLECULAR NEUROBIOLOGY
PMID：
36905568
Biocompatibility of Bacterial Magnetosomes as MRI Contrast Agent: A Long-Term In Vivo Follow-Up Study
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Author：
Xiaohui Nan, Wenjia Lai, Dan Li, Jiesheng Tian, Zhiyuan Hu, Qiaojun Fang
IF：
5.0760
Publish_to：
Nanomaterials
PMID：
34067162
Hydrogen sulfide alleviates the anxiety-like and depressive-like behaviors of type 1 diabetic mice via inhibiting inflammation and ferroptosis
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Author：
Yi Wang, Shengwen Wang, Yu Xin, Jinyue Zhang, Shaofan Wang, Zhuo Yang, Chunhua Liu
IF：
5.0370
Publish_to：
LIFE SCIENCES
PMID：
33945828
Brassinolide alleviates Fe deficiency-induced stress by regulating the Fe absorption mechanism in Malus hupehensis Rehd
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Author：
Sun Zhijuan, Guo Dianming, Lv Zhichao, Bian Chuanjie, Ma Changqing, Liu Xiaoli, Tian Yike, Wang Caihong, Zheng Xiaodong
IF：
4.9640
Publish_to：
PLANT CELL REPORTS
PMID：
35781542
Oxidative stress induces ferroptosis in tendon stem cells by regulating mitophagy through cGAS-STING pathway
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Author：
Yuanyuan Gao, Wenshuang Sun, Junrui Wang, Danli Zhao, Haoyuan Tian, Yangling Qiu, Shufan Ji, Shuqi Wang, Qiuyu Fu, Feng Zhang, Zili Zhang, Feixia Wang, Jiangjuan Shao, Shizhong Zheng, Jia Meng
IF：
4.8000
Publish_to：
INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：
38986301
FTY720 ameliorates experimental MPO-ANCA-associated vasculitis by regulating fatty acid oxidation via the neutrophil PPARα-CPT1a pathway
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Author：
Wang Rui-Xue, Wang Luo-Yi, Han Xiang-Yu, Chen Su-Fang, Sun Xiao-Jing, Li Zhi-Ying, Little Mark A, Zhao Ming-Hui, Chen Min
IF：
4.7000
Publish_to：
RHEUMATOLOGY
PMID：
38837706
Targeted treatment of rat AKI induced by rhabdomyolysis using BMSC derived magnetic exosomes and its mechanism
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Author：
Yuling Chen, Shike Hou
IF：
4.6000
Publish_to：
Nanoscale Advances
PMID：
39114150
Ferritinophagy-Mediated Hippocampus Ferroptosis is Involved in Cognitive Impairment in Immature Rats Induced by Hypoxia Combined with Propofol
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Author：
Liu Ling, Gao Wen, Yang Shun, Yang Fei, Li Shangyingying, Tian Yaqiong, Yang Li, Deng Qianyu, Gan Zhengwei, Tu Shengfen
IF：
4.4000
Publish_to：
NEUROCHEMICAL RESEARCH
PMID：
38512425
The protective mechanism of resveratrol against hepatic injury induced by iron overload in mice
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Author：
Jinghan Li, Simeng Wang, Jiaqi Duan, Peixin Le, Chao Li, Yongpei Ding, Rui Wang, Yonggang Gao
IF：
4.2190
Publish_to：
TOXICOLOGY AND APPLIED PHARMACOLOGY
PMID：
34044072
Resveratrol improves the iron deficiency adaptation of Malus baccata seedlings by regulating iron absorption
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Author：
Zheng Xiaodong, Chen Huifang, Su Qiufang, Wang Caihong, Sha Guangli, Ma Changqing, Sun Zhijuan, Yang Xueqing, Li Xiangyang, Tian Yike
IF：
4.2150
Publish_to：
BMC PLANT BIOLOGY
PMID：
34556040
Deferasirox exerts anti-epileptic effects by improving brain iron homeostasis via regulation of ITPRIP
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Author：
Xinhao Duan, Huifang Han, Jing Liu, Yu Qiu, Yi Wang, Xiaotian Wu, Hui Zhang, Zhen Zou, Jingfu Qiu, Chengzhi Chen, Fei Xiao, Xin Tian
IF：
4.2000
Publish_to：
NEUROCHEMISTRY INTERNATIONAL
PMID：
38561151
Isolation and Characterization of Vibrio kanaloae as a Major Pathogen Associated with Mass Mortalities of Ark Clam, Scapharca broughtonii, in Cold Season
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Author：
Bowen Huang, Xiang Zhang, Chongming Wang, Changming Bai, Chen Li, Chenghua Li, Lusheng Xin
IF：
4.1280
Publish_to：
Microorganisms
PMID：
34683482
A-lipoic acid alleviates DSS-induced ulcerative colitis via modulating Keap1-Nrf2 signaling pathway and inhibiting ferroptosis
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Author：
Peng Jiang, Zongzhen Zhai, Linxian zhao, Kai Zhang, Liwei Duan
IF：
4.1000
Publish_to：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
37850313
ErZhiTianGui Decoction alleviates age-related ovarian aging by regulating mitochondrial homeostasis and inhibiting ferroptosis
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Author：
Zhicheng Jia, Yongqian Li, Peixuan Wang, Kai Yang, Mengyu Shi, Wen Chen, Qihui Liang, Ying Guo
IF：
4.0000
Publish_to：
Journal of Ovarian Research
PMID：
38200521
Effect of iron overload on endothelial cell calcification and its mechanism
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Author：
Lili Zhao, Ning Yang, Yanqiu Song, Hailong Si, Qin Qin, Zhigang Guo
IF：
3.9320
Publish_to：
Annals of Translational Medicine
PMID：
34988167
Evaluation of the therapeutic efficacy of human bone marrow mesenchymal stem cells with COX-2 silence and TGF-β3 overexpression in rabbits with antigen-induced arthritis
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Author：
Tian He, Shui Sun
IF：
3.9050
Publish_to：
EXPERIMENTAL CELL RESEARCH
PMID：
34838812
Neuroprotective effects of morroniside from Cornus officinalis sieb. Et zucc against Parkinson’s disease via inhibiting oxidative stress and ferroptosis
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Author：
Li Mao, Zhang Junli, Jiang Lianyan, Wang Wujun, Feng Xianrong, Liu Meijun, Yang Dongdong
IF：
3.9000
Publish_to：
BMC Complementary Medicine and Therapies
PMID：
37393274
Reduction of TMAO level enhances the stability of carotid atherosclerotic plaque through promoting macrophage M2 polarization and efferocytosis
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Author：
Shi Weihao, Huang Yijun, Yang Zhou, Zhu Liang, Yu Bo
IF：
3.8400
Publish_to：
BIOSCIENCE REPORTS
PMID：
33969376
Dual targeting negative enrichment strategy for highly sensitive and purity detection of CTCs
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Author：
Gao Siying, Li Xuejie, Hu Zhiyuan, Wang Zihua, Hao Xiaopeng
IF：
3.8000
Publish_to：
Frontiers in Chemistry
PMID：
38831912
TmNAS3 from Triticum monococum directly regulated by TmbHLH47 increases Fe content of wheat grain
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Author：
Hongcheng Wang, Sisi Liao, Muzi Li, Jialian Wei, Bin Zhu, Lei Gu, Luhua Li, Xuye Du
IF：
3.6880
Publish_to：
GENE
PMID：
34864097
Accumulation of polyunsaturated lipids fuels ferroptosis to promote liver failure after extended hepatectomy in mice
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Author：
Can Huang, Jian Gan, Xiangyue Mo, Qingping Li, Leyi Liao, Biao Wang, Xianqiu Wu, Hanbiao Liang, Chen Xie, Tianzhou Peng, Yang Lei, Baoxiong Zhuang, Minghui Zeng, Yonghong Peng, Yisi Chen, Cuiting Liu, Jie Zhou, Kai Wang, Chuanjiang Li
IF：
3.6000
Publish_to：
FREE RADICAL RESEARCH
PMID：
39514464
Polydatin alleviates traumatic brain injury: Role of inhibiting ferroptosis
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Author：
Lu Huang, Shulei He, Qing Cai, Fei Li, Siwei Wang, Kai Tao, Ye Xi, Huaizhou Qin, Guodong Gao, Dayun Feng
IF：
3.5750
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
33839410
An integrated optical and chromogenic probe for tumor cell imaging
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Author：
Bin Zhang, Yadong Wang, Chao Xu, Yue Wang, Xiaolin Wang, Fuchao Wang, Yaling Wang, Hongzong Yin
IF：
3.5710
Publish_to：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
PMID：
35452914
Immunolipid magnetic bead-based circulating tumor cell sorting: a novel approach for pathological staging of colorectal cancer
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Author：
Qingyan Deng, Weidong Li, Yueming Huang, Haitao Wang, Xinhao Zhou, Zhifen Guan, Bohao Cheng, Yao Wang
IF：
3.5000
Publish_to：
Frontiers in Oncology
PMID：
39927117
Identification of JNK-JUN-NCOA axis as a therapeutic target for macrophage ferroptosis in chronic apical periodontitis
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Author：
Yuting Wang, Wenlan Li, Wenli Mu, Abdelrahman Seyam, Yonghui Guan, Yifei Tang, Mingfei Wang, Ying Xin, Xiaomei Guo, Tiezhou Hou, Xiaoyue Guan
IF：
3.2000
Publish_to：
International Journal of Medical Sciences
PMID：
39744165
SiO2 Induces Iron Overload and Ferroptosis in Cardiomyocytes in a Silicosis Mouse Model
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Author：
Yongheng Wang, Ning Li, Yi Guan, Tong LI, Yuxiu Zhang, Hong Cao, Zhihua Yu, Zhiheng Li, Shuoyan Li, Jiahao Hu, Wenxin Zhou, Sisi Qin, Shuang Li, Sanqiao Yao
IF：
3.0000
Publish_to：
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
PMID：
38988112
Magnetic transfection with superparamagnetic chitosan-loaded IGFBP5 nanoparticles and their in vitro biosafety
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Author：
Yue Tang, Jun Wu, Yuan Zhang, Lingpeng Ju, Xiangyang Qu, Dianming Jiang
IF：
2.9630
Publish_to：
Royal Society Open Science
PMID：
33614075
Bone marrow mesenchymal stem cells-derived exosomal lncRNA GAS5 mitigates heart failure by inhibiting UL3/Hippo pathway-mediated ferroptosis
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Author：
Ren Yu, Zhao Xingsheng
IF：
2.8000
Publish_to：
EUROPEAN JOURNAL OF MEDICAL RESEARCH
PMID：
38812041
The effect of magnetic guiding BMSCs on hypoxic-ischemic brain damage via magnetic resonance imaging evaluation
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Author：
Chuang Sun, Ning Zou, Honghai Chen, Aodan Zhang, Lianxin Sun, Zhengjuan Liu, Jie Bian
IF：
2.5460
Publish_to：
MAGNETIC RESONANCE IMAGING
PMID：
33727146
SIZ1 regulates phosphate deficiency-induced inhibition of primary root growth of Arabidopsis by modulating Fe accumulation and ROS production in its roots
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Author：
Zai Zheng, Dong Liu
IF：
2.2470
Publish_to：
Plant Signaling & Behavior
PMID：
34251993
Quercetin alleviates subarachnoid hemorrhage-induced early brain injury via inhibiting ferroptosis in the rat model
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Author：
Dian Jiao, Jianmiao Xu, Chengjian Lou, Yuhuan Luo, Chengtao Ni, Guanghong Shen, Marong Fang, Xiangyang Gong
IF：
2.2270
Publish_to：
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
PMID：
36437694
Progression and Regression of Abdominal Aortic Aneurysms in Mice
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Author：
Ding Yu-chao, Zhang Xian-jing, Zhang Ji-xiu, Zhai Zi-yi, Zhang Mei-xia, Jiang Bao-hong
IF：
2.1350
Publish_to：
Current Medical Science
PMID：
34643880
The Effects of Placental Mesenchymal Stem Cells Labeled With Ultrasmall Superparamagnetic Iron Oxides on the Growth of Colorectal Cancer Cells
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Author：
Wang Fang, He Hua, Xiong Yao, Hu Jie-ting, Guo Yu-lin
IF：
2.0810
Publish_to：
JOURNAL OF COMPUTER ASSISTED TOMOGRAPHY
PMID：
35995569
Krill Oil Ameliorates Liver Injury in Diabetic Mice by Activating Antioxidant Capacity and Inhibiting Ferroptosis
点击查看 >>
Author：
Huali Meng, Jie Li, Yu Yang, Yan Zheng, Shue Wang, Xin Guo, Lei Du, Hao Wu
IF：
1.6000
Publish_to：
Journal of Oleo Science
PMID：
39085082
Preparation and evaluation of LA-PEG-SPION, a targeted MRI contrast agent for liver cancer
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Author：
Lei Xia, Xiaowei Song, Guanghai Yan, Jishan Quan, Guangyu Jin
IF：
1.3110
Publish_to：
Open Life Sciences
PMID：
36045712
技术问答
（3）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
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您好，请问运动神经元细胞可以吗？染色时，直接在培养板中进行可以吗？2025-01-02 17:22:12
您好，此试剂盒适用于检测三价铁离子含量高的样本，如幼红细胞和铁纳米颗粒载药检测等，运动神经元细胞我司未进行过相关测试。如确认铁含量较高，您可以自行尝试在孔板内进行染色。如进行铁死亡相关变化检测建议使用增强法或相关荧光探针。
您好,染完拍照用普通光学显微镜就可以吗?2023-11-29 13:25:03
您好，可以的。
您好！请问贴壁细胞（家兔脂肪间充质干细胞）染色时，必须进行细胞爬片吗？可不可以直接在培养板中进行?2023-10-13 15:51:40
您好，可以的，根据实验需求选择合适孔板的大小，不是必须使用爬片，可以在孔板内染色，但是孔板内染色最后只能带水观察或使用水性封片剂封存，避免有机溶剂溶解板材。