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胃蛋白酶抑制剂

CAS:No.26305-03-3

英文名称:Pepstatin

分子式:C34H63N5O9

分子量:685.89

储存条件:Powder:-20℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:HPLC≥98%

胃蛋白酶抑制剂
满3999赠露营车
货号:
IP0170
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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是一种特异性的天冬氨酸蛋白酶 (aspartic proteases) 抑制剂,能够抑制 hemoglobin-pepsin,hemoglobin-proctase,casein-pepsin,casein-proctase,casein-acid protease 和 hemoglobin-acid protease 的活性; 同时可抑制 HIV protease 的活性。


In Vitro

CellHEK293T cells hESCs1 μg/mL Pepstatin30 min at 4◦C on a rotation wheel

For TFAP2C-OTX2 Co-immunoprecipitation, HEK293T cells expressing either HA-tagged OTX2 and MYC-tagged TFAP2C protein were collected after being scraped, pelleted, and washed three times using 1 × PBS. The cells were lysed with RIPA buffer supplemented with a cocktail of protease inhibitors (1 μg/mL Aprotinin (Solarbio, IA0430), 1 μg/mL Leupeptin (Solarbio, IL0070), 1 μg/mL Pepstatin (Solarbio, IP0170) and 1 mM PMSF) for 30 min at 4◦C on a rotation wheel. The soluble cell lysates were collected after maximum speed centrifugation at 4◦C for 30 min. Subsequently, 1 μg/mL of HA beads was incubated with cell lysates for 2 hrs at 4◦C on a rotation wheel. Beads were then washed for three times with PBS. Proteins were eluted in 1 × SDS loading buffer for subsequent Western Blot analysis. For MacroH2A.1-OTX2, MacroH2A.1-TFAP2C Co-immunoprecipitation, control hESCs expressing either 3 × FLAG-tagged OTX2 and MacroH2A.1 or control and OTX2-KO hESCs expressing 3 × FLAG-tagged OTX2 and MacroH2A.1 protein were collected after being scraped, pelleted, and washed three times using 1 × PBS. The cells were lysed with RIPA buffer supplemented with a cocktail of protease inhibitors (1 μg/mL Aprotinin (Solarbio, IA0430), 1 μg/mL Leupeptin (Solarbio, IL0070), 1 μg/mL Pepstatin (Solarbio, IP0170) and 1 mM PMSF) for 30 min at 4◦C on a rotation wheel. The soluble cell lysates were collected after maximum speed centrifugation at 4◦C for 30 min. Subsequently, 1 μg/mL of FLAG antibody with beads and 1 μg/mL of MacroH2A.1 antibody with beads were incubated with cell lysates for 2 hrs at 4◦C on a rotation wheel. Beads were then washed for three times with PBS. Proteins were eluted in 1 × SDS loading buffer for subsequent Western Blot analysis.

文献来源:Zhang J, Gu Y, Tong L, Feng B, Dong S, Shao Q, Chen Y, Tu H, Wang Z, Wang Y, Li X, Yu H, Lin Z, Wang X, Li Z, Ai Z, Xiang Y, Jiang Z, Jin Z, Li Z, Chen Y, Shen Z, Huang C, Liu J, Liu J, Xu P, Yu Y, Xia P, Liang H, Huang H, Chen D. IGF2BP1 restricts the induction of human primordial germ cell fate in an m6A-dependent manner. Cell Stem Cell. 2025 May 21:S1934-5909(25)00181-X. doi: 10.1016/j.stem.2025.05.001. Epub ahead of print. PMID: 40436019.


备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
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Zhang J,et al.Cell Stem Cell. 2025 May 21:S1934-5909(25)00181-X. doi: 10.1016/j.stem.2025.05.001.
Zhang J,et al.Cell Stem Cell. 2025 May 21:S1934-5909(25)00181-X. doi: 10.1016/j.stem.2025.05.001.
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