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活动
> 二乙酸荧光素
二乙酸荧光素

CAS:No.596-09-8

英文名称:Fluorescein Diacetate

分子式:C24H16O7

分子量:416.38

储存条件:Powder:-20℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year

纯度:HPLC≥98%

  IF0160说明书
二乙酸荧光素
满3999赠露营车
货号：
IF0160
品牌：
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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IF0160-D-1ml 咨询 咨询 咨询 咨询
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产品简介 相关产品 相关论文 COA 技术问答

是一种用于活体染色的荧光探针，常与PI联用，用于检测细胞活力和细胞毒性等。Ex=494nm / Em=521nm

FDA是一种非荧光性疏水性荧光素衍生物，它可以穿透细胞膜进入细胞，通过细胞内酯酶催化水解二乙酸酯基团产生具有高强度的荧光产物荧光素。荧光分子在具有完整细胞膜的细胞内积累，因此这种绿色荧光可以作为一种细胞活力的标签。不具有完整细胞膜或者新陈代谢活性的细胞，不能在细胞内积累荧光产物，因此不能显现绿色荧光。FDA可以与染料PI联合使用，无活性的死细胞被PI着色，发出红色荧光；有活性的细胞PI无法着色，在FDA作用下，紧发出绿色荧光。这两种颜色可以很好的辨别死细胞与活细胞，与单一颜色检测相比，它提供了一种更为准确的活细胞定量检测方法。


使用本产品的案例（仅供参考）

In Vitro

Cell （KB cells；5μg/mL FDA；15s）

PI and FDA co-staining experiments evaluated the effect of PTT in vitro.   KB cells were cultured with Au NCs in 6-well plate at concentration of 100μg/mL based on the content of Au, and then irradiated by 660 nm laser with laser power density of 2 W/cm2 for different times of 0, 5, 10 and 15 min. FDA of 5μg/mL was used to stain the living cells, and the staining time was 15 s.   Meanwhile, PI of 5 μg/mL was used to stain the apoptosis cells, and the staining time was 15 min.

来源文献：Chen Q, Guan G, Deng F, Yang D, Wu P, Kang S, Sun R, Wang X, Zhou D, Dai W, Wang X, Zhang H, He B, Chen D, Zhang Q. Anisotropic active ligandations in siRNA-Loaded hybrid nanodiscs lead to distinct carcinostatic outcomes by regulating nano-bio interactions. Biomaterials. 2020 Apr 3;251:120008. doi: 10.1016/j.biomaterials.2020.120008. Epub ahead of print. PMID: 32388031.


Cell（PC12 cell；10 μg/mL FDA；15 min）

PC12 cells were added to the 6-well plate with a density of 1 × 105 cells/well. Cells were cultured at 37 °C for 24 h with 5% CO2. The preincubated Aβ samples with or without 2.00 mg/mL ulvan were added to the cells and cultured for an additional 48 h. After the medium was gently aspirated with a pipet gun, cells with good adherence were incubated with 10 μg/mL FDA and 5 μg/mL PI for 15 min. Finally, the FDA/PI staining solution was removed, andn the cells were washed three times with PBS buffer and observed using a BX53 fluorescence microscope.

来源文献：Liu F, Zhao W, Zhao F, Dong Q, Wang Y, Wei W, Jia L, Li L, Lu F. Dual Effect of the Acidic Polysaccharose Ulvan on the Inhibition of Amyloid-β Protein Fibrillation and Disintegration of Mature Fibrils. ACS Appl Mater Interfaces. 2020 Sep 16;12(37):41167-41176. doi: 10.1021/acsami.0c14292. Epub 2020 Sep 1. PMID: 32818379.


P. ternata seedling(Pinellia ternata root tip cell;  15 μg/mL FDA;  10min)

We accurately weigh 100 mg of fluorescein diacetate (FDA) (Solarbio, Beijing, China) and dissolved it in 4 mL of acetone to prepare a 25 mg·mL-1 mother liquor, which was stored away from light at -20 °C.  When in use, FDA storage solution was added to 0.65 mol·L-1 mannitol to obtain FDA dyeing solution in proportion.  10 mg of propidium iodide (PI) (Solarbio, China) was dissolved in 2 mL of 0.65 mol·L-1 mannitol to prepare a 5 mol·L-1 mother liquor.  The FDA (15 μg·mL-1) and PI (5 μg·mL-1) mixtures were used to treat P. ternata seedlings.  P. ternata seedlings were hydroponically cultured and treated with M-Pa-B for 1, 2, 3, and 4 h. After that, the root tips of seedlings were washed three times with deionized water and then dyed with the FDA-PI in the dark for 10 min. The excess dye was washed away thoroughly with deionized water after dyeing.  Ten root samples were collected from five individual plants at each time point.  A laser confocal scanning microscope was used for observation and photography.  The excitation and emission wavelengths are 488 nm and 630 nm, respectively.

来源文献：He Z, Wang Y, Yan Y, Qin S, He H, Mao R, Liang Z. Dynamic analysis of physiological indices and transcriptome profiling revealing the mechanisms of the allelopathic effects of phenolic acids on Pinellia ternata.  Front Plant Sci. 2022 Oct 18;13:1039507.  doi: 10.3389/fpls.2022.1039507.  PMID: 36340387;  PMCID: PMC9635339.


Cell（L929 mouse fibroblast cells,stained with FDA and PI reagent at 1 mg/mL）

After 1, 4 days of culture, the medium was discarded, and L929 cells were washed with PBS and stained with FDA and PI reagent at 1 mg/mL.After incubating in the dark for 5 min, the cells were washed with PBS solution again.

来源文献：Cao X, Ma L, Tan Y, Tong Q, Liu D, Yi Z, Li X. Soft yet mechanically robust injectable alginate hydrogels with processing versatility based on alginate/hydroxyapatite hybridization. Int J Biol Macromol. 2024 Jun;270(Pt 2):132458. doi: 10.1016/j.ijbiomac.2024.132458. Epub 2024 May 19. PMID: 38772458.


Cell（MC3T3-E1 cell, Following incubation for 1, 3, and 5 days, live/dead cell staining experiments were conducted by using FDA and PI reagents）

a cell suspension with a density of 5 × 104 was carefully pipetted on the sterilized matrices that were placed in 24-well plates for cocultivation. Following incubation for 1, 3, and 5 days, live/dead cell staining experiments were conducted by using FDA and PI reagents. The stained cells were then observed and photographed on a laser scanning confocal microscope (LSCM) with a product model number of LSM 880.

来源文献：Tan Y, Ma L, Cao X, Yi Z, Ma X, Li X. Tunable Stress Relaxing Biomimetic Matrices: Hyaluronan/Hydroxyapatite Hybridization Mediates Assembly of Collagen Fibrils. Biomacromolecules. 2023 Nov 13;24(11):5162-5174. doi: 10.1021/acs.biomac.3c00718. Epub 2023 Oct 27. PMID: 37889885.



备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
暂无详情
Yang F, et al. J Biomed Mater Res A. 2022 Jan;110(1):31-42. doi: 10.1002/jbm.a.37263.
Yao S, et al.[J]. Frontiers in Medical Science Research, 2021, 3(1).
Shi H, et al. Progress in Organic Coatings, 2021, 159: 106422.
Liu F, et al. ACS Appl Mater Interfaces. 2020 Sep 16;12(37)41167-41176.
He Z, et al. Front Plant Sci. 2022 Oct 18;13:1039507.
Cao X, et al. Int J Biol Macromol. 2024 Jun;270(Pt 2):132458. doi: 10.1016/j.ijbiomac.2024.132458.
Tan Y, et al. Biomacromolecules. 2023 Nov 13;24(11):5162-5174. doi: 10.1021/acs.biomac.3c00718.
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3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
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