中文
英语
碱性磷酸酶染色试剂盒(改良Gomori钙钴法)
英文名称:Alkaline Phophatase Stain Kit(Modified Gomori Ca-CoS Method)
储存条件:2-8℃，避光保存，有效期6个月。
产品简介
| 中文名称 | 碱性磷酸酶染色试剂盒(改良Gomori钙钴法) |
| 英文名称 | Alkaline Phophatase Stain Kit(Modified Gomori Ca-CoS Method) |
| 储存条件 | 2-8℃，避光保存，有效期6个月。 |
| 规格 | 3*50ml |
| 单位 | 盒 |
碱性磷酸酶(alkaline phosphatase，简称ALP或AKP)为一类磷酸酯酶，广泛分布于哺乳动物组织内，其活性所需最适pH 9.2~9.8。此酶主要存在于物质交换活跃之处（细胞膜），如肠上皮和肾近曲小管的刷状缘、附睾上皮之静纤毛、肝的毛细胆管膜以及微动脉和毛细血管动脉部之内皮。此酶还见于内质网、高尔基复合体、吞饮小泡、肠上皮之溶酶体、中性粒细胞之中性颗粒以及平滑肌之细胞膜及吞饮小泡。
我司的碱性磷酸酶染色试剂盒(改良Gomori钙钴法)使用金属沉淀法来显示碱性磷酸酶活性，有级联放大效应，可定性或半定量检测冰冻和石蜡切片中的碱性磷酸酶分布。此法以天然存在的β-甘油磷酸钠为底物，经酶水解释放出磷酸，立即被金属离子捕获转为沉淀，再依次被置换为棕黑色稳定附着色淀,便于观察和长期保存。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
大鼠肾脏冰冻切片
阳性位点着棕褐色近黑，细胞核着红色，背景着浅红色或无色
阳性位点着棕褐色近黑，细胞核着红色，背景着浅红色或无色
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Yee Michelle Min-Fang, Chin Kok-Yong, Ima-Nirwana Soelaiman, Alias Ekram, Chua Kien Hui, Wong Sok Kuan
IF：
3.2000
Publish_to：
International Journal of Medical Sciences
PMID：
39898246
The mechanism of lncRNA MALAT1 targeting the miR-124-3p/IGF2BP1 axis to regulate osteogenic differentiation of periodontal ligament stem cells
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Author：
Gu Nan, Wang Yao, Li Lingfeng, Sui Xin, Liu Zhihui
IF：
3.1000
Publish_to：
Clinical Oral Investigations
PMID：
38492123
Vitamin D Alleviates Cadmium-Induced Inhibition of Chicken Bone Marrow Stromal Cells＊ Osteogenic Differentiation In Vitro
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Author：
Xishuai Tong, Ying Zhang, Yutian Zhao, Yawen Li, Tan Li, Hui Zou, Yan Yuan, Jianchun Bian, Zongping Liu, Jianhong Gu
IF：
3.0000
Publish_to：
Animals
PMID：
37570352
A novel technique to harvest bone autografts with mild local hyperthermia and enhanced osteogenic bone quality: a preclinical study in dogs
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Author：
Zhou Tengfei, Gan Zekun, Zhang Hanfei, Liu Ziyi, Pu Yiping, Rong Mingdeng
IF：
2.9000
Publish_to：
BMC Oral Health
PMID：
37936153
Novel-miR-81 Promotes the Chondrocytes Differentiation of Bone Marrow Mesenchymal Stem Cells Through Inhibiting Rac2 Expression
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Author：
Ziwei Luo, Jinqi Xie, Haoxiang Ye, Jie Zhang, Yangping Liu, Chunmei Ma, Jiahui Cao, Hao Pan, Xiaosheng Liu, Xianxi Zhou, Jiechen Kong, Dongfeng Chen, Aijun Liu
IF：
2.8000
Publish_to：
Cartilage
PMID：
37997349
The recombinant BMP-2 loaded silk fibroin microspheres improved the bone phenotype of mild osteogenesis imperfecta mice
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Author：
Ting Fu, Yi Liu, Zihan Wang, Yaqing Jing, Yuxia Zhao, Chenyi Shao, Zhe Lv, Guang Li
IF：
2.7000
Publish_to：
PeerJ
PMID：
37927786
Whole serum as cell protection reagent in cell shipment at low or room temperature
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Author：
Daijiang Liu, Dianliang Fang, Wen Li, Xiaoqiang Wan, Lei Lang, Zhenyan Wang
IF：
2.5860
Publish_to：
TISSUE & CELL
PMID：
36481581
Platelet-rich plasma-derived exosomes promote rotator cuff tendon-bone healing
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Author：
Lei Han, Ningrui Hu, Canfeng Wang, Zhengcong Ye, Tuo Wang, Fang Lan
IF：
2.5000
Publish_to：
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
PMID：
37984013
Total glycosides from Eucommia ulmoides seed promoted osteogenic differentiation of adipose-derived mesenchymal stem cells and bone formation in ovariectomized rats through regulating Notch signaling pathway
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Author：
Zhou Yu-hu, Xie Qiang
IF：
2.3590
Publish_to：
Journal of Orthopaedic Surgery and Research
PMID：
34742334
LncRNA MEG3 promotes osteogenesis of hBMSCs by regulating miR-21-5p / SOD3 axis
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Author：
Shuangli Wang, Gaoxin Xiong, Rende Ning, Zhengjun Pan, Meihua Xu, Zhengang Zha, Ning Liu
IF：
2.3490
Publish_to：
ACTA BIOCHIMICA POLONICA
PMID：
35231166
Ameliorative Effects of Extracellular Vesicles Derived from Mesenchymal Stem Cells on Apoptosis and Differentiation of Osteoblasts Treated with CoCl2
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Author：
Qicheng Li, Wei Zhang, Jin Deng, Qiuya Li, Xiaoyang Fu, Yuhui Kou, Na Han
IF：
2.2570
Publish_to：
Cellular Reprogramming
PMID：
37184657
Analysis of ceRNA networks during mechanical tension-induced osteogenic differentiation of periodontal ligament stem cells
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Author：
Wenfang Wang, Ning Li, Meijuan Wang, Yunshan Zhao, Hao Wu, Jingyi Shi, Mazen Musa, Xi Chen
IF：
2.1600
Publish_to：
EUROPEAN JOURNAL OF ORAL SCIENCES
PMID：
35969187
Effect of Tensile Frequency on the Osteogenic Differentiation of Periodontal Ligament Stem Cells
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Author：
Wenfang Wang, Meijuan Wang, Xiaomei Guo, Yunshan Zhao, Madiha Mohammed Saleh Ahmed, Hong Qi, Xi Chen
IF：
2.1450
Publish_to：
International Journal of General Medicine
PMID：
35811779
Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells
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Author：
Lin Wei, Hou Lianglei, Tang Jialyu, Huang Anwu, Jia Zhuyin
IF：
2.1000
Publish_to：
BMC Cardiovascular Disorders
PMID：
38654161
OCT4, SOX2 and NANOG co-regulate glycolysis and participate in somatic induced reprogramming
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Author：
Ding Ying, Yuan Xia, Zou Yichen, Gao Jiachen, Xu Xianshuai, Sun Hongyan, Zuo Qisheng, Zhang Yani, Li Bichun
IF：
2.0400
Publish_to：
CYTOTECHNOLOGY
PMID：
35733702
Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development
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Author：
Yumei Luo, Yapei Chen, Mimi Zhang, Xiaoyan Ma, Detu Zhu, Yaoyong Chen
IF：
2.0200
Publish_to：
Stem Cell Research
PMID：
34710837
A comprehensive stem cell laboratory module with blended learning for medical students at Tongji University
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Author：
Jie Xu, Zhihua Shao, Song Jia, Jihong Sha, Jiao Li, Furong Gao, Xiujuan Shi, Juan Wang, Caixia Jin, Mei Jiang, Haibin Tian, Jinfeng Cao, Hu Pu, Lei Xu, Lixia Lu
IF：
1.4000
Publish_to：
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
PMID：
38189805
技术问答
（3）
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可以提供一份染细胞的操作说明书吗？2025-03-21 09:41:28
您好，目前暂时没法提供，具体步骤可参考切片步骤
染细胞的操作方法有吗?2024-01-04 21:57:01
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请问这个试剂盒能测贴壁细胞吗?2023-03-13 10:13:52
您好，可以的。