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去氧胆酸钠

CAS:No.302-95-4

英文名称:Deoxycholic acid sodium salt

纯度:HPLC≥98%

分子式:C24H39O4Na

分子量:414.56

储存条件:Store at 2-8℃,2 years.

去氧胆酸钠
货号:
SD8820
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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使用本产品的案例(仅供参考)


LC-MS/MS

Analyzed on a UltiMate 3000 UHPLC system coupled with an Orbitrap Q Exactive HF mass spectrometer. A 90-min separation gradient was established with mobile phase A (2% ACN, 0.1% FA) and mobile phase B (0.1% FA in ACN) (0-8 min, 3%-6% B;8-9 min, 6% B;9-25 min, 6%-12% B;25-60 min, 12%-24% B;60-70 min, 24%-35% B;70-76 min, 35%-90% B;76-85 min, 90% B;85-86 min,90%-6% B;86-90 min, 6% B), where the detection time for the MS was 25-85 min, and therefore also referred to as the 60 min effective gradient. Peptides were ionized at 2 kV. The MS was operated in DIA mode with an m/z scan range of 350 to 1,500 and a resolution of 60,000. The maximum injection time (IT), automatic gain control (AGC) target, and inclusion list were set to 20 ms,1 ×106, and 40 windows, respectively. High energy collisional dissociation (HCD) was employed for precursor ion fragmentation,where the normalized collision energy was 28. The maximum ion injection time of the MS2 was set to automatic and the resolution was 30,000.

来源文献:Ma C, Li Y, Li J, Song L, Chen L, Zhao N, Li X, Chen N, Long L, Zhao J, Hou X, Ren L, Yuan X. Comprehensive and deep profiling of the plasma proteome with protein corona on zeolite NaY. J Pharm Anal. 2023 May;13(5):503-513. doi: 10.1016/j.jpha.2023.04.002. Epub 2023 Apr 12. PMID: 37305782;PMCID: PMC10257194.


RP-HPLC

The released hippuric acid (HA) was quantified by RP-HPLC (Allicance 2695, Waters, MA, USA) with a SunfireTM-C18 column (150 mm × 4.6 mm). The injection volume was 20 μL and the flow rate was 0.4 ml min−1 using a mobile phase of ultra-pure (Milli-Q) water (Millipore, Bedford, MA, USA), acetonitrile, and trifluoroacetic acid (TFA) mixed in a volume ratio of 55 : 45 : 0.05. The HA of every sample was monitored at 228 nm.

来源文献:Gong KJ, Shi AM, Liu HZ, Liu L, Hu H, Yang Y, Adhikari B, Wang Q. Preparation of nanoliposome loaded with peanut peptide fraction: stability and bioavailability. Food Funct. 2016 Apr;7(4):2034-42. doi: 10.1039/c5fo01612f. PMID: 27007221.

备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

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Ma C, et al. J Pharm Anal. 2023 May;13(5):503-513.
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1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
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